Effect of Baicalin on the Expression of Nuclear Factor κB/IκBα Induced by Hydrogen Peroxide in Astrocytes

SONG Yuan-Zong, YANG Yu-Jia, JIA Yan

Chinese Journal of Contemporary Pediatrics ›› 2002, Vol. 4 ›› Issue (6) : 436-438.

PDF(243 KB)
PDF(243 KB)
Chinese Journal of Contemporary Pediatrics ›› 2002, Vol. 4 ›› Issue (6) : 436-438.
ORIGINAL ARTICLE

Effect of Baicalin on the Expression of Nuclear Factor κB/IκBα Induced by Hydrogen Peroxide in Astrocytes

  • SONG Yuan-Zong, YANG Yu-Jia, JIA Yan
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Abstract

OBJECTIVE: To investigate the effect of baicalin, a flavonoid extracted from the radix of  Scutellaria baicalensis Georgi, on the expression of nuclear factor (NF) κB/IκBα induced by hydrogen peroxide (H 2O 2) in astrocytes. METHODS: Astrocytes were cultured in vitro, and treated with various concentrations of baicalin or/and H 2O 2. Immunocytochemistry was used to determine the translocation of P65 from cytoplasm to nucleus; and Western Blot was used to determine the expression change of IκBα in astrocytes. RESULTS: In astrocytes, H 2O 2 could induce the translocation of P65, a subunit of NF κB, from cytoplasm to nucleus, and reduce the level of IκBα expression, while baicalin could inhibit this effect. CONCLUSIONS:Baicalin may decrease the degree of IκBα degradation and NF κB activation induced by H 2O 2 in astrocytes.

Key words

Astrocytes / Baicalin / Hydrogen peroxide / Nuclear factor κB / IκBα

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SONG Yuan-Zong, YANG Yu-Jia, JIA Yan. Effect of Baicalin on the Expression of Nuclear Factor κB/IκBα Induced by Hydrogen Peroxide in Astrocytes[J]. Chinese Journal of Contemporary Pediatrics. 2002, 4(6): 436-438
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