Effect of ketamine combined with penehyclidine hydrochloride on the expression of synaptophysin in the brain of neonatal rats

LIN Lei, ZHANG Liang-Cheng, GUO Yong-Zheng

Chinese Journal of Contemporary Pediatrics ›› 2010, Vol. 12 ›› Issue (1) : 51-55.

PDF(1411 KB)
PDF(1411 KB)
Chinese Journal of Contemporary Pediatrics ›› 2010, Vol. 12 ›› Issue (1) : 51-55.
EXPERIMENTAL RESEARCH

Effect of ketamine combined with penehyclidine hydrochloride on the expression of synaptophysin in the brain of neonatal rats

  • LIN Lei, ZHANG Liang-Cheng, GUO Yong-Zheng
Author information +
History +

Abstract

OBJECTIVE: To study the effects of ketamine combined with penehyclidine hydrochloride on the learning and memory abilities and the expression of synaptophysin in the hippocampus CA3 region in the brain of neonatal rats. METHODS: Eighty seven-day-old Sprague-Dawly rats were randomly intraperitoneally injected with 50 mg/kg of ketamine (K group), 2 mg/kg of penehyclidine hydrochloride (P group), 50 mg/kg of ketamine plus 2 mg/kg penehyclidine hydrochloride (PK group) or normal saline (control group). The rats were trained and tested in a Morris water maze 14 days after administration. The immunhistochemical method was used to ascertain the expression of synaptophysin in the hippocampus CA3 region 24 hrs, 14 days and 28 days after administration. RESULTS: In the Morris water maze training, the rats in the PK group performed worst, followed by the K group. The rats from the P and NS groups performed well. Compared with the NS group, the expression of synaptophysin in the K and the PK groups decreased significantly 24 hrs and 14 days after administration (P<0.05). The PK group had lower synaptophysin expression than the K group 24 hrs and 14 days after administration (P<0.05). Up to 28 days after administration, the synaptophysin expression increased in all of the four groups and there were no significant differences between groups. CONCLUSIONS: Ketamine combined with penehyclidine hydrochloride may inhibit more significantly learning and memory abilities and the synaptophysin expression in the hippocampus CA3 region than ketamine alone in neonatal rats. Penehyclidine hydrochloride alone has no effect on learning and memory abilities and the synaptophysin expression. The synaptophysin expression may increase to a normal level by training and with increasing age.[Chin J Contemp Pediatr, 2010, 12 (1):51-55]

Key words

Ketamine / Penehyclidine hydrochloride / Learning and memory / Synaptophysin / Neonatal rats

Cite this article

Download Citations
LIN Lei, ZHANG Liang-Cheng, GUO Yong-Zheng. Effect of ketamine combined with penehyclidine hydrochloride on the expression of synaptophysin in the brain of neonatal rats[J]. Chinese Journal of Contemporary Pediatrics. 2010, 12(1): 51-55

References

[1]刘毓和,吴新民,杜敏逸.咪唑安定、异丙酚和氯胺酮对老年大鼠认知功能的影响[J].中华麻醉学杂志, 2006, 26(4):315-317.
[2]李凤鹏,郑健.脑内胆碱能烟碱受体与认知功能的关系[J].临床神经病学杂志, 2004, 17(3):236-238.
[3]关新民.医学神经生物学纲要[M].北京:科学出版社, 2003:52.
[4]纵雪梅,庞涛,许铁,耿德勤,梁高永,燕宪亮.盐酸戊乙奎醚预处理对大鼠脑缺血/再灌注损伤所致脑梗死体积和神经元凋亡的影响[J].国际麻醉学与复苏杂志, 2006, 27(6):334-337.
[5]武云飞,王志萍,江山,曾因明.多次氯胺酮麻醉对发育早期大鼠空间辨别学习记忆与海马 CA1区突触长时程增强的影响[J].徐州医学院学报, 2005, 25(3):221-224.
[6]陈志斌,杨天德.氯胺酮对SD幼鼠海马神经元细胞凋亡和突触素表达的影响[J].第三军医大学学报, 2005, 27(20):2042-2044.
[7]马智聪,杨建新,马平.长托宁和东莨菪碱用作高血压患者术前用药的比较研究[J].中华新医学, 2004, 5(9):812-813.
[8]朱广球,尤匡掌.麻醉前使用戊乙奎醚对老年人术后早期精神功能的影响[J].中国新药与临床杂志, 2002, 26(4):280-283.
[9]彭博.盐酸戊乙奎醚致精神症状2例[J].医药导报, 2007, 26(7):814.
[10]李茜,蔡舒,顾嘉宾.盐酸戊乙奎醚合用氯胺酮对小鼠学习记忆的影响[J].徐州医学院学报, 2007, 27(9):606-608.
[11]宿宝贵,潘三强.大鼠海马结构在空间辨别性学习记忆时突触素表达的变化[J].中国病理生理杂志, 2000, 16(5):421-423.
[12]Gomez-Pinilla F,So V,Kesslak JP.Spatial learning induces neurotrophin receptor and synapsin I in the hippocampus[J]. Brain Res, 2001, 904(1):13-19.
[13]潘三强,宿宝贵,韩辉,吕来清,李仲购,周丽丽,等.大鼠海马CA3区在学习记忆时突触可塑性的变化[J].解剖学杂志, 2001, 24(4):336-339.
[14]Wang C, Anastasio N, Popov V, Leday A, Johnson KM. Blockade of N-methyl-D-aspartate receptors by phencyclidine causes the loss of corticostriatal neurons[J].Neuroscience, 2004, 125(2):473-483.
[15]Olney JW. New insights and new issues in developmental neurotoxicology[J].Neurotoxicology, 2002, 23(6):659-668.
[16]初春芹,桂波,陈玲.盐酸戊乙奎醚对老龄大鼠认知功能的影响[J].临床麻醉学杂志, 2008, 24(4):328-330.
[17]袁力勇,戴体俊,王霞民.咪达唑仑与盐酸戊乙奎醚对小鼠学习记忆获得和巩固及再现的影响[J].医药导报, 2007, 27(1):7-10.
[18]文敏,周波,康朝胜,臧贵勇,王景传,余资江.大鼠海马 CA3区突触素的增龄性变化[J].山东大学学报(医学版), 2008, 46(3):229-231.
[19]宋文秀,曹云涛,刘华庆.新生鼠脑缺血再灌注后海马CA1 区突触素的表达及意义[J].第四军医大学学报,2004,25(16):1467-1469.
[20]Kleim JA, Jones TA, Schallert T. Motor enrichment and the induction of plasticity before or after brain injury[J]. Neurochem Res, 2003, 28 (11):1757-1769.
[21]Chen YC, Chen QS, Lei JL, Wang SL. Physical training modifies the age-related decrease of GAP-43 and synaptophysin in the hippocampal formation in C57BL/6J mouse[J].Brain Res, 1998, 806(2):238-245.
[22]Becker JT, Walker JA, Olton DS. Neuroanatomical bases of spatial memory[J].Brain Res, 1980, 200(2):307-320.
[23]O′Keefe J, Speakman A. Single unit activity in the rat hippocampus during a spatial memory task[J].Exp Brain Res, 1987, 68(1):1-27.
[24]蒯建科,孙绪德,高昌俊.氯胺酮对新生大鼠认知功能和海马突触素表达的影响[J].医学研究生学报, 2008, 21(4):364-366.

PDF(1411 KB)

Accesses

Citation

Detail

Sections
Recommended

/