Expression of stromal cell derived factor-1 and CXC chemokine receptor 4 and the effects of budesonide on their expression in mice with asthma

LUAN Bin; HUANG Xian-Jie; QIAO Jun-Ying

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Chinese Journal of Contemporary Pediatrics ›› 2010, Vol. 12 ›› Issue (3) : 215-218.

Expression of stromal cell derived factor-1 and CXC chemokine receptor 4 and the effects of budesonide on their expression in mice with asthma

LUAN Bin, HUANG Xian-Jie, QIAO Jun-Ying

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Abstract

OBJECTIVE: To study the expression of stromal cell derived factor-1(SDF-1) and CXC chemokine receptor 4 (CXCR4) in the airway and the effect of budesonide on their expression in mice with asthma. METHODS: Thirty BALB/c male mices were randomly divided into three groups: placebo control, untreated asthma, and budesonide-treated asthma. The asthma group were induced by intraperitoneal injection of 10% ovalbumin (OVA ) on days 1, 8 and 15, and then from days 22 to 34, challenged by inhalation of 2% OVA aerosol every other day. The budesonide-treated asthma group received an inhalation of budesonide (1 mg ) before OVA challenge. The pathological changes of the airway were assessed by hematoxylin and eosin staining. The immunohistochemistry was used to estimate the expression of SDF-1 in the lung. RT-PCR was used to evaluate the expression of CXCR4 in the lung. RESULTS: Compared with the control group, SDF-1 and CXCR4 expression in the lung in the untreated asthma group increased significantly (P<0.05). The budesonide-treated asthma group demonstrated significantly decreased SDF-1 (0.426±0.052 vs 0.361±0.065; P<0.05) and CXCR4 (0.829±0.027 vs 0.723±0.094; P<0.05) expression in the lung as compared with the untreated asthma group. Both SDF-1 (r=0.744, P<0.01) and CXCR4 (r=0.553, P<0.01）were positively correlated with the thickness of the airway wall. CONCLUSIONS: SDF-1 and CXCR4 may be associated with airway remodeling in mice with asthma. Budesonide can improve airway remodeling, possibly by decreasing the expression of SDF-1 and CXCR4.［Chin J Contemp Pediatr, 2010, 12 (3):215-218］

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Asthma / Airway remodeling / Stromal cell derived factor-1 / CXC chemokine receptor 4 / Mice

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LUAN Bin, HUANG Xian-Jie, QIAO Jun-Ying. Expression of stromal cell derived factor-1 and CXC chemokine receptor 4 and the effects of budesonide on their expression in mice with asthma[J]. Chinese Journal of Contemporary Pediatrics. 2010, 12(3): 215-218

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