Effect of oral glutamine on intestinal barrier function in yaung rats with endotoxemia

CHANG Xiao; WANG Lin-Lin; LIAN Shu-Jun; TANG Qing; CHEN Ping; WANG Hua

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Chinese Journal of Contemporary Pediatrics ›› 2010, Vol. 12 ›› Issue (10) : 809-811.

EXPERIMENTAL RESEARCH

Effect of oral glutamine on intestinal barrier function in yaung rats with endotoxemia

CHANG Xiao, WANG Lin-Lin, LIAN Shu-Jun, TANG Qing, CHEN Ping, WANG Hua

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Abstract

OBJECTIVE: To study the effect of glutamine on intestinal barrier function by examining the changes of plasma D-lactic levels and diamine oxidase (DAO) levels in plasma and intestinal tissue after glutamine intervention in young rats with endotoxemia. METHODS: Eighty 18-day-old rats were randomly divided into endotoxemia and glutamine intervention groups (n=40 each). Endotoxemia was induced by lipopolysaccharide (LPS) injection. Plasma and small intestine homogenate were collected 1.5, 6, 24 and 72 hrs and 7 days after LPS injection. The glutamine intervention group was immediately administered with oral glutamine (2 g/kg) after LPS injection. Afterwards, glutamine was administered once daily. Plasma D-lactic and DAO levels and intestinal DAO levels were measured. RESULTS: Plasma DAO activity in the glutamine intervention group was significantly lower than that in the endotoxemia group 6 and 72 hrs after LPS injection (P<0.05). In contrast, the intestinal DAO activity in the glutamine intervention group was significantly higher than that in the endotoxemia group 6, 24 and 72 hrs and 7 days after LPS injection (P<0.05 or 0.01). Plasma D-lactic levels in the glutamine intervention group were significantly lower than those in the endotoxemia group 6, 24 and 72 hrs and 7 days after LPS injection (P<0.01). CONCLUSIONS: Glutamine may reduce the permeability of intestinal mucosa, and thus provides protective effects on intestinal barrier function in rats with endotoxemia.［Chin J Contemp Pediatr, 2010, 12 (10):809-811］

Key words

Glutamine / D-lactate / Diamine oxidase / Endotoxemia / Young rats

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CHANG Xiao, WANG Lin-Lin, LIAN Shu-Jun, TANG Qing, CHEN Ping, WANG Hua. Effect of oral glutamine on intestinal barrier function in yaung rats with endotoxemia[J]. Chinese Journal of Contemporary Pediatrics. 2010, 12(10): 809-811

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