Effect of astrocytes with different degrees of proliferation on multidrug resistance gene expression in rats with epilepsy

CHEN Xiao-Cong; HUANG Shao-Ping; WANG Xue-Ying

PDF(1183 KB)

Chinese Journal of Contemporary Pediatrics ›› 2010, Vol. 12 ›› Issue (11) : 908-911.

Effect of astrocytes with different degrees of proliferation on multidrug resistance gene expression in rats with epilepsy

CHEN Xiao-Cong, HUANG Shao-Ping, WANG Xue-Ying

Author information +

History +

Abstract

OBJECTIVE: To study the relationship of activated astrocytes and multidrug resistance gene (MDR) expression in rats with epilepsy. METHODS: Astrocytes of neonatal Sprague-Dawley rats were separated and cultured. The cultured cells of passage 3 were activated by TNF-α for 2, 24 or 48 hrs. The culture media of cells with different degrees of proliferation were infused to the lateral cerebral ventricle of rats with epilepsy. The expression of MDR in the brain tissue was ascertained by PCR, immunocytochemistry and Western blot. RESULTS: After 2 hrs of TNF-α stimulation, astrocytes began to proliferate, and reached a peak at 24 hrs. The expression of MDR in the brain tissue increased after infusion of culture medium of proliferated astrocytes in the TNF stimulation group compared with that in the control group without TNF stimulation. The level of MDR expression in the TNF stimulation group was positively correlated with the degrees of cell proliferation. CONCLUSIONS: Proliferation of astrocytes can increase the expression of MDR in rats with epilepsy and is probably involved in the development of refractory epilepsy.［Chin J Contemp Pediatr, 2010, 12 (11):908-911］

Key words

Astrocyte / Proliferation / Multidrug resistance gene / Epilepsy / Rats

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

CHEN Xiao-Cong, HUANG Shao-Ping, WANG Xue-Ying. Effect of astrocytes with different degrees of proliferation on multidrug resistance gene expression in rats with epilepsy[J]. Chinese Journal of Contemporary Pediatrics. 2010, 12(11): 908-911

References

［1］Lazarowski A，Massaro M，Schteinschnaider A，Intruvini S，Sevlever G，Rabinowicz A. Neuronal MDR-1 gene expression and persistent low levels of anticonvulsants in a child with refractory epilepsy［J］. Ther Drug Monit, 2004, 26 (1): 44-46.
［2］Golden PL, Pardridge WM. Brain microvascular p-glycoprotein and a revised model of multidrug resistance in brain［J］. Cell Mol Neurobiol, 2000, 20(2): l65-181.
［3］Sisodiya SM, Lin WR, Squier MV，Thom M. Multidrug-resistance protein 1 in focal cortical dysplasia［J］. Lancet, 2001, 357(9249):42-43.
［4］Sisodiya SM, Lin WR, Harding BN, Squier MV, Thom M. Drug resistance in epilepsy: expression of drug resistance proteins in common causes of refractory epilepsy［J］. Brain, 2002, 125(1):22-31.
［5］丁成云，徐群渊，栾国明，杨慧，王元身. MDR-1和GFAP蛋白在难治性癫癎脑组织的表达［J］.中华神经外科杂志，2002, 18(40), 238-240.
［6］Marchi N, Hallene KL, Kight KM, Cucullo L, Moddel G, Bingaman W. Significance of MDR1 and multiple drug resistance in refractory human epileptic brain［J］. BMC Med, 2004, 2（10）:37-49.
［7］Christin E. Pharmacoresistance: Modem concept and basic date derived from human brain tissue［J］. Epilepsia, 2003, 44 (5): 9-15.
［8］Potschka H, Fedrowitz M, Loscher W. P-glycoprotein and multidrug resistance-associated protein are involved in the regulation of extracellular levels of the major antiepileptic drug carbamazepine in the brain［J］. Neurpharmacol Neurotoxical, 2001, 12(16):3557-3560.
［9］Potschka H, Loscher W. In vivo evidence for P-glycoprotein-ediated transport of phenytoin at the blood-brain barrier of rats［J］. Epilepsia, 2001, 42(10):1231-1240.
［10］Qu H, Eloqayli H, Muller B, Aasly J, Sonnewald U. Glial-neuronal interactions following kainite injection in rats［J］. Neurochem Int, 2003, 42(1):101-106.
［11］McCarthy KD，Devellis J． Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue［J］. J Cell Biol, 1980, 85(3):890-902.
［12］李丹,黄绍平,马伟军,张婕. 氯化锂-匹鲁卡品癫癎动物模型的建立［J］.陕西医学杂志，2006，1（35）：15-17.
［13］包新民, 舒斯云.大鼠脑立体定位图谱［M］. 北京:人民卫生出版社，1991.
［14］Haspolat S, Mihci E, Coskun M, Gümüslü S, Ozben T, Yegin O. Interleukin-1beta, tumor necrosis factor-alpha, and nitrite levels in febrile seizures［J］. Child Neurol, 2002, 17(10):749-751.
［15］Diehi JA, Tong W, Sun G, Hannink M. Tumor necrosis factor-alpha-dependent activation of a RelA homodimer in astrocytes.Increased phosphorylation of RelA and MAD-3 precede activation of RelA［J］. J Biol Chem, 1995, 270 (6):2703-2707.