Abstract OBJECTIVE: To study the effect of oxymatrine (Oxy) on airway inflammation and the distribution of dendritic cells (DC) in lung and spleen tissues of asthmatic mice. METHODS: Fifty BALB/c mice were assigned into five groups (n=10): an asthma model group, a dexamethasone (Dex) treatment group and three Oxy treatment groups (Oxy dose: 20, 40 and 80 mg/kg respectively). The histological changes of lung tissues were observed by hematoxylin and eosin staining. The expression of 33D1 antigen (a marker of DC) in lung and spleen tissues were detected by immunohistochemical staining. RESULTS: The inflammatory reactions of the lung tissues in the Dex or Oxy treatment groups were less severe than those in the asthma model group. 33D1 antigen was remarkably expressed in the lung and spleen tissues of the asthma model group. After Dex treatment, the expression of 33D1 antigen in the lung and spleen tissues decreased significantly (P<0.01). 33D1 antigen expression in the lung tissues was significantly reduced in all of the three Oxy treatment groups in a dose-dependent manner compared with that in the asthma model group (P<0.01). The treatment with Oxy of 40 and 80 mg/kg decreased significantly the 33D1 antigen expression in the spleen tissues (P<0.01). CONCLUSIONS: Oxy can alleviate airway inflammation and reduce the number of DC in lung and spleen tissues of asthma mice, which may be contributed to the mechanism of Oxy for treatment of asthma.
HOU Wei,LIU Hai-Yan,YANG Xu-Dong et al. Effect of oxymatrine on the distribution of dendritic cells in lung and spleen tissues of asthmatic mice[J]. 中国当代儿科杂志, 2011, 13(1): 40-43.
HOU Wei,LIU Hai-Yan,YANG Xu-Dong et al. Effect of oxymatrine on the distribution of dendritic cells in lung and spleen tissues of asthmatic mice[J]. CJCP, 2011, 13(1): 40-43.
［8］Agger R, Crowley MT, Witmer-Pack MD. The surface of dendritic cells in the mouse as studied with monoclonal antibodies［J］ Int Rev Immunol, 1990, 6(2-3): 89-101
［9］Lombardi V, Akbari O. Dendritic cell modulation as a new interventional approach for the treatment of asthma［J］. Drug News Perspect, 2009, 22(8): 445-451.
［10］Grunig G, Banz A, de Waal Malefyt R. Molecular regulation of Th2 immunity by dendritic cells［J］. Pharmacol Ther, 2005, 106(1): 75-96.
