Effects of TNF-α on ICAM-1 and LFA-1 expression in peripheral blood mononuclear cells of children with febrile seizures

SUN Dan, LIU Zhi-Sheng, YAO Hui, KANG Shi-Xiu, HE Cai-Ying, HU Jia-Sheng, WU Ge-Fei, WANG Fang-Lin

Chinese Journal of Contemporary Pediatrics ›› 2011, Vol. 13 ›› Issue (4) : 285-287.

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Chinese Journal of Contemporary Pediatrics ›› 2011, Vol. 13 ›› Issue (4) : 285-287.
CLINICAL RESEARCH

Effects of TNF-α on ICAM-1 and LFA-1 expression in peripheral blood mononuclear cells of children with febrile seizures

  • SUN Dan, LIU Zhi-Sheng, YAO Hui, KANG Shi-Xiu, HE Cai-Ying, HU Jia-Sheng, WU Ge-Fei, WANG Fang-Lin
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Abstract

OBJECTIVE: To study the effects of TNF-α on ICAM-1 and LFA-1 expression in peripheral blood mononuclear cells (PBMC) of children with febrile seizures (FS). METHODS: Sixteen children with FS and 16 age- and gender-matched healthy children were enrolled. The samples of PBMC from FS children were randomized into two groups with or without TNF-α treatment (TNF-α concentration 1.0 ng/mL). PBMC were purified and cultured with a conventional method in vitro. The expression of ICAM-1 and LFA-1 in PBMC was determined by flow cytometry (FCM). RESULTS: ICAM-1[(20±9)% vs (14±7)%)]and LFA-1[(43±16)% vs (30±16)%]expression in PBMC in the untreated FS group was significantly higher than that in the normal control group (P<0.05). Compared with the untreated FS group, the treatment with TNF-α remarkably increased the ICAM-1 expression[(27±11)%](P<0.05). PBMC LFA-1 expression[(52±21)%]in the TNF-α-treated group was higher than that in the untreated FS group, although there were no statistical differences between the two groups. CONCLUSIONS: TNF-α treatment may increase LFA-1 and ICAM-1 expression in PBMC of children with FS.

Key words

TNF-α / Febrile seizure / ICAM-1 / LFA-1 / Peripheral blood mononuclear cell / Child

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SUN Dan, LIU Zhi-Sheng, YAO Hui, KANG Shi-Xiu, HE Cai-Ying, HU Jia-Sheng, WU Ge-Fei, WANG Fang-Lin. Effects of TNF-α on ICAM-1 and LFA-1 expression in peripheral blood mononuclear cells of children with febrile seizures[J]. Chinese Journal of Contemporary Pediatrics. 2011, 13(4): 285-287

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