Protective effects of lidocaine on hyperoxia-exposed type Ⅱalveolar epithelial cells from premature rats

DING Xiao-Fang; ZHONG Li-Li; ZHANG Bing; LI Jia

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Chinese Journal of Contemporary Pediatrics ›› 2011, Vol. 13 ›› Issue (4) : 313-316.

EXPERIMENTAL RESEARCH

Protective effects of lidocaine on hyperoxia-exposed type Ⅱalveolar epithelial cells from premature rats

DING Xiao-Fang, ZHONG Li-Li, ZHANG Bing, LI Jia

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Abstract

OBJECTIVE: To investigate the effects of lidocaine on apoptosis and proliferation of hyperoxia-exposed type Ⅱalveolar epithelial cells (AECⅡ) from premature rats. METHODS: Primary cultured AEC Ⅱ isolated from premature rats were randomly divided into four groups: air, air+ lidocaine (20 μg/mL), 95%O2/5%CO2, and 95%O2/5%CO2+ lidocaine. The cells in each group were collected 24 hrs after culture in ordinary incubators (37℃,5%CO2). The proliferation and apoptosis of AEC Ⅱ were detected by flow cytometry. Protein levels of proliferating cell nuclear antigen (PCNA) were measured by Western blot. RESULTS: The apoptosis rate of AECⅡincreased, the percentages of G2/M and S phase cells decreased and the protein levels of PCNA decreased significantly in the group exposed to 95%O2/5%CO2 compared with the group exposed to air (P<0.01). Lidocaine treatment decreased apoptosis rate of AECⅡ, increased percentage of G2/M and S phase cells, and increased protein levels of PCNA. CONCLUSIONS: Hyperoxia can increase apoptosis and inhibit proliferation of AECⅡ in premature rats. Lidocaine may have protective effects against the AECⅡ injuries.

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DING Xiao-Fang, ZHONG Li-Li, ZHANG Bing, LI Jia. Protective effects of lidocaine on hyperoxia-exposed type Ⅱalveolar epithelial cells from premature rats[J]. Chinese Journal of Contemporary Pediatrics. 2011, 13(4): 313-316

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