Abstract OBJECTIVE: To investigate the effects of matrine on the proliferation and apoptosis of human rhabdomyosarcoma RD cells in vitro, and to explore the mechanism of matrine inducing apoptosis of RD cells. METHODS: MTT assay was used to measure the proliferation inhibition rates of RD cells that were treated with matrine (final concentrations= 0.5, 1.0, 1.5, and 2.0 mg/mL). Flow cytometry was used to evaluate the apoptosis of RD cells treated with the four concentrations of matrine. RT-PCR was used to measure the mRNA expression of cyclin D1 and survivin in RD cells treated with 0.5, 1.0, and 1.5 mg/mL of matrine. RESULTS: The RD cells treated with various concentrations of matrine showed significantly higher proliferation inhibition rates and apoptotic rates than those that were not treated with matrine (P<0.01), and with increased matrine concentration, the proliferation inhibition rate of RD cells increased gradually, thus exhibiting a dose dependence. The mRNA expression of cyclin D1 and survivin was seen in all RD cells, but was significantly lower in RD cells treated with matrine than in those that were not treated with matrine (P<0.01). There were significant differences in cyclin D1 mRNA level among the RD cells treated with 0.5, 1.0, and 1.5 mg/mL of matrine (P<0.05), while there was significant difference in survivin mRNA level between the RD cells treated with 0.5 and 1.5 mg/mL of matrine (P<0.05). CONCLUSIONS: Matrine can significantly inhibit proliferation and induce apoptosis of RD cells, which may be related to downregulating the mRNA expression of cyclin D1 and survivin.
GUO Lei,XUE Tian-Yang,XU Wei et al. Effects of matrine on the proliferation and apoptosis of human rhabdomyosarcoma RD cells[J]. 中国当代儿科杂志, 2012, 14(10): 780-784.
GUO Lei,XUE Tian-Yang,XU Wei et al. Effects of matrine on the proliferation and apoptosis of human rhabdomyosarcoma RD cells[J]. CJCP, 2012, 14(10): 780-784.
［1］Ognjanovic S, Linabery AM, Charbonneau B, Ross JA. Trends in childhood rhabdomyosarcoma incidence and survival in the United States 19752005［J］. Cancer, 2009, 115(18): 4218-4226.
［2］Liu J, Manheimer E, Tsutani K, Gluud C. Medicinal herbs for hepatitis C virus infection: a Cochrane hepatobiliary systematic review of randomized trials［J］. Am J Gastroenterol, 2003, 98(3): 538-544.
［3］Chen K, Hu Z, Wang T, Guo H, Ye Z. Inhibitory effect of matrine on the expression of PSA and AR in prostate cancer cell line LNCaP［J］. Huazhong Univ Sci Technolog Med Sci, 2008, 28(6): 697-699.
［4］Liu XY, Fang H, Yang ZG, Wang XY, Ruan LM, Fang DR, et al. Matrine inhibits invasiveness and metastasis of human malignant melanoma cell line A375 in vitro［J］. Int J Dermatol, 2008, 47(5): 448-456.
［6］Li HJ, Xia HB. Regulation of tumor angiogenesis and tumor therapy［J］. J Mod Oncol, 2008, 16(7): 1245-1248.
［7］Donskow-Schmelter K, Doligalska M. Apoptosis, a protective mechanism for pathogens and their hosts［J］. Wiad Parazytol, 2005, 51(4) :271-280.
［8］Udhayakumar G, Jayanthi V, Devaraj N, Devaraj H. Interaction of MUC1 with beta-catenin modulates the Wnt target gene cyclinD1 in H.pylori-induced gastric cancer［J］. Mol Carcinog, 2007, 46 (9): 807-817.
［9］Krecicki T, Smigiel R, Fraczek M, Kowalczyk M, Sasiadek MM . Studies of the cell cycle regulatory proteins P16, cyclin D1 and retinoblastoma protein in laryngeal carcinoma tissue［J］. J Laryngol Otol, 2004, 118(9): 676-680.
［10］Guha M, Altieri DC. Survivin as a global target of intrinsic tumor suppression networks［J］. Cell Cycle, 2009, 8 (17): 2708-2710.
［11］Ryan BM, O′Donovan N, Duffy MJ. Survivin: a new target for anti-cancer therapy［J］. Cancer Treat Rev, 2009, 35(7): 553-562.
［12］Shin S, Sung BJ, Cho YS, Kim HJ, Ha NC, Hwang JI, et al . An anti-apoptotic protein human survivin is a direct inhibitor of caspase -3 and-7［J］. Biochemistry, 2001, 40(4): 1117-1123.
