Abstract OBJECTIVE: To examine the levels of nerve growth factor (NGF) and interleukin-4 (IL-4) in the induced sputum of children with cough variant asthma (CVA), with the aim of studying the roles of NGF and IL-4 in childhood CVA. METHODS: Thirty-four children with CVA were enrolled in this study. Twenty healthy children were used as a normal control group. The induced sputum was separated into supernatant and cells. Hematoxylin and eosin staining was used to count differential cells. The expression of NGF and IL-4 in supernatant was measured using ELISA. The mRNA expression of NGF and IL-4 in cells was determined by Real-time PCR analysis. RESULTS: The percentage of eosinophils in the CVA group was significantly higher than in the control group [(13.4±3.6)% vs (2.6±1.7)%; P<0.01]. The expression of NGF and IL-4 protein and mRNA in induced sputum was significantly higher in the CVA group than in the control group (P<0.05). The expression of NGF and IL-4 protein and mRNA was positively correlated with the percentage of eosinophils (P<0.01). The expression of NGF and IL-4 protein and mRNA in induced sputum was significantly reduced in the CVA group after treatment (P<0.05). CONCLUSIONS: Eosinophils infiltration and increased expression of NGF and IL-4 play key roles in the development of childhood CVA, suggesting that they may be useful in the diagnosis and treatment of childhood CVA.
LI Hong-Zi,JIN Zheng-Yong,YUAN Xi-Zhe et al. Levels of nerve growth factor and interleukin-4 in the induced sputum of children with cough variant asthma[J]. 中国当代儿科杂志, 2012, 14(12): 924-927.
LI Hong-Zi,JIN Zheng-Yong,YUAN Xi-Zhe et al. Levels of nerve growth factor and interleukin-4 in the induced sputum of children with cough variant asthma[J]. CJCP, 2012, 14(12): 924-927.
［2］Takemura M, Niimi A, Matsumoto H, Ueda T, Yamaguchi M, Matsuoka H, et al. Atopic features of cough variant asthma and classic asthma with wheezing［J］. Clin Exp Allergy, 2007, 37(12): 1833-1839.
［3］Matsumoto H, Niimi A, Tabuena RP, Takemura M, Ueda T, Yamaguchi M, et al. Airway wall thickening in patients with cough variant asthma and nonasthmatic chronic cough［J］. Chest, 2007, 13l(4): 1042-1049.
［4］Curtis JL. Cell-mediated adaptive immune defense of the lungs［J］. Proc Am Thorac Soc, 2005, 2(2): 412-416
［8］Matsumoto H, Niimi A, Takemura M, Ueda T, Yamaguchi M, Matsuoka H, et al. Features of cough variant asthma and classic asthma during methacholine-induced brochoconstriction: a cross-sectional study［J］. Cough, 2009, 5: 3.
［9］Freund-Michel V, Bertrand C, Frossard N. TrkA signalling pathways in human airway smooth muscle cell proliferation［J］. Cell Signal, 2006, 18(5): 621-627.
［10］El-Hashim AZ, Jaffal SM. Nerve growth factor enhances cough and airway obstruction via TrkA receptor-and TRPVl-dependent mechanisms［J］. Thorax, 2009, 64(9): 79l-797.
［11］Meng L, He X, Zhu W, Yang X, Jiang C, Sun Q, et al. TLR3 and TLR7 modulate IgE production in antigen induced pulmonary inflammation via influencing IL-4 expression in immune organs［J］. PLoS One, 2011, 6(2): e17252.
［12］Nassenstein C, Braun A, Erpenbeck VJ, Lommatzsch M, Schmidt S, Krug N, et al. The neurotrophins nerve growth factor, brain-derived neurotrphic factor, neurotrophin-3, and neurotrophin-4 are survival and activation factors for eosinophils in patients with allergic bronchial asthma［J］. J Exp Med, 2003, 198(3): 455-467.
