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CD96 expression on leukemia stem cells in 69 children with acute leukemia
WANG Xue-Mei, YAN Mei, LIU Yu, HAILIQIGULI Nu-Er-Mai-Mai-Ti
Chinese Journal of Contemporary Pediatrics ›› 2013, Vol. 15 ›› Issue (8) : 633-637.
PDF(1351 KB)
PDF(1351 KB)
CD96 expression on leukemia stem cells in 69 children with acute leukemia
OBJECTIVE: To detect the expression of surface molecule CD96 on stem cell (LSC) in children with acute leukemia, and to explore its clinical significance. METHODS: Bone marrow mononuclear cells were isolated in 69 children with newly diagnosed acute leukemia. CD34+CD38-CD123+ LSCs were separated from these cells by flow cytometry (FCM) and then cultured, and CD96 expression on LSCs was detected by FCM. R-banding technique was used to analyze the karyotypes of the 69 children, and the data of their routine blood and immunological tests were collected. RESULTS: CD96 was mainly expressed in children with acute myelogenous leukemia, and expressed to a lesser extent in those with acute lymphoblastic leukemia (P<0.05). The median expression level of CD96 in Uyghur children was 23.4%, versus 21.2% in Han children (P>0.05). The majority of children with CD96-positive children presented poor-prognosis karyotypes. Compared with CD96-negative children, children with CD96-positive children had a significantly lower complete remission rate (P<0.05) and significantly higher infection and relapse rates after chemotherapy (P<0.05). CONCLUSIONS: Children with acute leukemia who have CD96-positive LSCs have a poor prognosis. CD96 may be a new indicator of prognosis in children with acute leukemia.
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