Expression of myeloid-related protein complex in association with circulating endothelial cells in children with acute Kawasaki disease

WANG Cong-Jun; CHEN Mei; LEI Zhong-Jing

doi:10.7499/j.issn.1008-8830.2014.01.011

PDF(1618 KB)

Chinese Journal of Contemporary Pediatrics ›› 2014, Vol. 16 ›› Issue (1) : 48-52. DOI: 10.7499/j.issn.1008-8830.2014.01.011

CLINICAL RESEARCH

Expression of myeloid-related protein complex in association with circulating endothelial cells in children with acute Kawasaki disease

WANG Cong-Jun¹, CHEN Mei¹, LEI Zhong-Jing²

Author information +

History +

Abstract

Objective To investigate the expression of myeloid-related protein complex (MRP-8/14) in children with acute Kawasaki Disease (KD). Methods A total of 41 children with acute KD and 40 age-and sex-matched control children with upper respiratory tract infection were recruited. Serum levels of MRP-8/MRP-14 complex were measured by ELISA, messenger ribonucleic acid (mRNA) abundance of MRP-8 and MRP-14 in circulating granulocytes and monocytes was determined by RT-PCR, and the number of circulating endothelial cells was determined by flow cytometry. Results When the analysis was stratified according to the presence or absence of coronary artery ectasia in the KD patient group, serum levels of MRP-8/MRP-14 complex, MRP-8 and MRP-14 mRNA abundance in granulocytes, and the number of circulating endothelial cells were all significantly higher in KD patients with coronary artery ectasia than in KD patients without coronary artery ectasia (P<0.05). Serum levels of MRP-8/MRP-14 complex were positively correlated with the number of endothelial cells in the circulation (r=0.69, P<0.05). Conclusions Serum levels of MRP-8/MRP-14 complex are elevated in a positive association with the number of circulating endothelial cells in KD children with coronary artery ectasia, suggesting a causative role in the development of coronary artery lesions.

Key words

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

WANG Cong-Jun, CHEN Mei, LEI Zhong-Jing. Expression of myeloid-related protein complex in association with circulating endothelial cells in children with acute Kawasaki disease[J]. Chinese Journal of Contemporary Pediatrics. 2014, 16(1): 48-52 https://doi.org/10.7499/j.issn.1008-8830.2014.01.011

References

[1] Robinson MJ, Tessier P, Poulsom R, et al. The S100 family heterodimer, MRP-8/14, binds with high affinity to heparin and heparan sulfate glycosaminoglycans on endothelial cells[J]. J Biol Chem, 2002, 277(5): 3658-3665.
[2] Pepper RJ, Hamour S, Chavele KM, et al. Leukocyte and serum S100A8/S100A9 expression reflects disease activity in ANCA-associated vasculitis and glomerulonephritis[J]. Kidney Int, 2013, 83(6): 1150-1158.
[3] Newburger JW, Takahashi M, Gerber MA, et al. Diagnosis,treatment, and long-term management of Kawasaki disease: a statement for health professionals from the Committee on Rheumatic Fever, Endocarditis and Kawasaki Disease, Council on Cardiovascular Disease in the Young, American Heart Association[J]. Circulation, 2004, 110(17): 2747-2771.
[4] 李焰, 王献民, 柳颐龄, 等. 川崎病患儿并发冠状动脉病变的危险因素分析[J]. 中国当代儿科杂志, 2012, 14(12): 938-941.
[5] 段超, 杜忠东, 王玉, 等. 川崎病冠脉瘤患儿远期血管内皮功能的研究[J]. 中国当代儿科杂志, 2011, 13(5): 373-376.
[6] Roth J, Vogl T, Sorg C, et al. Phagocyte-specific S100 proteins: a novel group of proinflammatory molecules[J]. Trends Immunol, 2003, 24(4): 155-158.
[7] Maiseyeu A, Badgeley MA, Kampfrath T, et al. In vivo targeting of inflammation-associated myeloid-related protein 8/14 via gadolinium immunonanoparticles[J]. Arterioscler Thromb Vasc Biol, 2012, 32(4): 962-970.
[8] Holzinger D, Frosch M, Kastrup A, et al. The Toll-like receptor 4 agonist MRP8/14 protein complex is a sensitive indicator for disease activity and predicts relapses in systemic-onset juvenile idiopathic arthritis[J]. Ann Rheum Dis, 2012, 71(6): 974-980.
[9] Manolakis AC, Kapsoritakis AN, Tiaka EK, et al. Calprotectin, calgranulin C, and other members of the S100 protein family in inflammatory bowel disease[J]. Dig Dis Sci, 2011, 56(6): 1601-1611.
[10] Viemann D, Barczyk K, Vogl T, et al. MRP8/MRP14 impairs endothelial integrity and induces a caspase-dependent and independent cell death programn[J]. Blood, 2007, 109(6): 2453-2460.
[11] Donato R. Intracellular and extracellular roles of S100 proteins[J]. Microsc Res Tech, 2003, 60(6): 540-551.
[12] Bertolini F, Shaked Y, Mancuso P. On the clinical relevance of circulating endothelial cells and platelets in prostate cancer[J]. Br J Cancer, 2013, 108(6): 1387.
[13] Lampka M, Grabczewska Z, Jendryczka-Mackiewicz E, et al. Circulating endothelial cells in coronary artery disease[J]. Kardiol Pol, 2010, 68(10): 1100-1105.
[14] Campioni D, Zauli G, Gambetti S, et al. In vitro characterization of circulating endothelial progenitor cells isolated from patients with acute coronary syndrome[J]. PLoS One, 2013, 8(2): e56377.
[15] Erdbruegger U, Dhaygude A, Haubitz M, et al. Circulating endothelial cells: markers and mediators of vascular damage[J]. Curr Stem Cell Res Ther, 2010, 5(4): 294-302.