Abstract Objective To investigate the single nucleotide polymorphisms (SNPs) at interleukin 6 (IL-6)-174 and TNF-β NcoI in Chinese Han children in Guangzhou, China and to provide basic information for study on the association between IL-6-174 and TNF-β NcoI polymorphisms and systemic inflammatory response syndrome (SIRS). Methods Allele-specific polymerase chain reaction and polymerase chain reaction-restriction fragment length polymorphism were used to determine the SNPs at IL-6-174 and TNF-β NcoI in 481 children selected from the Han population in Guangzhou in 2012. Genotype analysis and comparison with other populations were made with reference to relevant literature. Results Chinese Han children in Guangzhou had only GG genotype at IL-6-174, and the SNP at this locus was rare or not seen in the Han population in Guangzhou. At TNF-β NcoI, the frequencies of TNF-β 1*1, TNF-β 1*2, and TNF-β 2*2 genotypes were 24.7%, 49.7%, and 25.6%, respectively. The sample distribution was in accordance with Hardy- Weinberg equilibrium. The TNF-β 1 allele frequency was significantly higher in Guangzhou Han population than in European and American white population (PConclusions TNF-β NcoI SNP is prevalent in the Han population in Guangzhou, and the distribution of alleles is significantly different from that in the white population. The sample from an Hardy-Weinberg equilibrium population can be further used for study on the association between TNF-β NcoI SNP and SIRS in Chinese Han children in Guangzhou. IL-6-174 SNP is rare or not seen in the Han population in Guangzhou, so SNP at this locus cannot be selected for disease association analysis.
WEI Yan-Dan,ZENG Hua-Song. Analysis of single nucleotide polymorphisms at IL-6-174 and TNF-β NcoI in Chinese Han children in Guangzhou, China[J]. CJCP, 2014, 16(8): 846-850.
WEI Yan-Dan,ZENG Hua-Song. Analysis of single nucleotide polymorphisms at IL-6-174 and TNF-β NcoI in Chinese Han children in Guangzhou, China[J]. CJCP, 2014, 16(8): 846-850.
Beutler B, Poltorak A. Sepsis and evolution of the innate immune response[J]. Crit Care Med, 2001, 29(7 Suppl): S2-S7.
[3]
d a S i l v a C o r r e i a J , S o l d a u K , C h r i s t e n U , e t a l . Lipopolysaccharide is in close proximity t o each of the proteins in its membrane recept or complex. transfer from CD14 to TLR4 and MD-2 [J]. J Biol Chem, 2001, 276(24): 21129-21135.
[4]
Remick DG, Bologos G, Coperand S, et al. Role of interleukin -6 in mortality from and physiologic response to sepis [J]. Infect Immun, 2005, 73(5): 2751-2757.
[5]
Schotte H, Schlüter B, Rust S, et al. Interleukin-6 promoter polymorphism (-174G/C) in Caucasian German patients with systemic lupus erythematosus[J]. Rheumatology, 2001, 40(4): 393-400.
[6]
Majetschak M, Obertacke U, Schade FU, et al. Tumor necrosis factor gene polymorphisms, leukocyte function, and sepsis susceptibility in blunt trauma patients[J]. Clin Diagn Lab Immunol, 2002, 6(9): 1205-1211.
[7]
Pohjanen VM, Koivurova OP, Mäkinen JM, et al. Interleukin 6 Gene polymorphism -174 is associated with the diffuse type gastric carcinoma[J]. Genes Chromosomes Cancer, 2013, 52(10): 976-982.
[8]
Asano NM, Angelo HD, da Silva HA, et al. Interleukin-6 promoter polymorphisms -174 G/C in Brazilian patients with systemic lupus erythematosus[J]. Human Immunol, 2013, 74(9): 1153-1156.
[9]
Gharagozlou M, Farhadi E, Khaledi M, et al. Association Between the Interleukin 6 genotype at position -174 and atopic dermatitis[J]. J Investig Allergol Clin Immunol, 2013, 2(23): 89- 93.
[10]
Ryu JH, Kim SJ. Interleukin-6 -634 C/G and -174 G/C polymorphisms in Korean patients undergoing hemodialysis[J]. Korean J Intern Med, 2012, 3(27): 327-337.
Tong Y, Wang Z, Geng Y, et al. The association of functional polymorphisms of IL-6 gene promoter with ischemic stroke: analysis in two Chinese populations[J]. Biochem Biophys Res Commun, 2010, 391(1): 481-485.
Um JY, Park JH, Kim HM. Gene polymorphisms in tumor necrosis factor locus and waist-hip ratio in obese Koreans[J]. Clinica Chimica Acta, 2003, 338(1/2): 117-122.
[17]
Hayakawa T, Nagai Y, Taniguchi M, et al. Tumor necrosis factor-b gene NcoI polymorphism decreases insulin resistance in Japanese Men[J]. Metabolism, 2000, 49(11): 1506-1509.
[18]
Asuni C, Stochino ME, Cherchi A, et al. Migraine and tumour necrosis factor gene polymorphism[J]. J Neurol, 2009, 256(2): 194-197.
[19]
Ates O, Müsellim B, Ongen G, et al. Analysis of TNF polymorphisms in turkish systemic sclerosis patients with interstitial lung involvement[J]. Biochem Genet, 2008, 46(11/12): 696-701.
[20]
Garnacho-Montero J, Aldabo-Pallas T, Garnacho-Montero C, et al. Timing of adequate antibiotic therapy is a greater determinant of outcome than are TNF and IL-10 polymorphisms in patients with sepsis[J]. Critical Care, 2006, 10(4): R111.
[21]
Majetschak M, Flohé S, Obertacke U, et al. Relation of a TNF Gene polymorphism to severe sepsis in trauma patients[J]. Ann Surgery, 1999, 2(230): 207-214.
