Effects of glucocorticoid on RAGE-NF-&kappa;B pathway in hyperoxia-induced lung tissues of neonatal rats

HU Jian; YU Min; TANG Yun; TIAN Zhao-Fang

doi:10.7499/j.issn.1008-8830.2015.01.018

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Chinese Journal of Contemporary Pediatrics ›› 2015, Vol. 17 ›› Issue (1) : 81-85. DOI: 10.7499/j.issn.1008-8830.2015.01.018

EXPERIMENTAL RESEARCH

Effects of glucocorticoid on RAGE-NF-κB pathway in hyperoxia-induced lung tissues of neonatal rats

HU Jian, YU Min, TANG Yun, TIAN Zhao-Fang

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Abstract

Objective To explore the change of RAGE-NF-κB signaling pathway during the course of hyperoxiainduced lung injury in newborn rats, and the effect of glucocorticoid on this pathway. Methods Twenty-four Sprague- Dawley neonatal rats were randomly divided into three groups (n=8 each): sham control (control group), hyperoxiainduced acute lung injury (model group) and glucocorticoid-treated acute lung injury (glucocorticoid group). Rats were sacrificed at 13 days after birth. RAGE and NF-κB expression levels in lung tissues were detected by reverse transcription polymerase chain reaction, Western blot and immunohistochemistry analysis. The levels of tumor necrosis factor α (TNF-α) and sRAGE in bronchoalveolar lavage fluid (BALF) and serum were measured using ELISA. Lung damage was evaluated by histological examinations. Results RAGE and NF-κB mRNA and protein expression levels in lung tissues were significantly increased in the model and glucocorticoid groups compared with the control group (P<0.05). Serum RAGE concentrations were significantly increased but RAGE concentrations in BALF were significantly reduced in the model and glucocorticoid groups compared with the control group (P<0.05). RAGE and NF-κB expression at both mRNA and protein levels in lung tissues was significantly lower in the glucocorticoid group than in the model group (P<0.05). RAGE concentrations were significantly lower in serum (P<0.05), but were higher in BALF (P<0.05) in the glucocorticoid group than in the model group. Conclusions RAGE-NF-κB pathway plays an important role in hyperoxia-induced lung injury in neonatal rats, and glucocorticoid administration may play a protective role against the lung injury by down-regulating RAGE-NF-κB signaling pathway.

Key words

Glucocorticoid / Receptor for advanced glycation end-products / Lung injury / Neonatal rats

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HU Jian, YU Min, TANG Yun, TIAN Zhao-Fang. Effects of glucocorticoid on RAGE-NF-κB pathway in hyperoxia-induced lung tissues of neonatal rats[J]. Chinese Journal of Contemporary Pediatrics. 2015, 17(1): 81-85 https://doi.org/10.7499/j.issn.1008-8830.2015.01.018

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