Plasma proteomic analysis in children with infectious mononucleosis

RAN Zhi-Ling; XIAO Bin; LIU Hong-Rui; LIU You-Ping; SHENG Qiao-Ni

doi:10.7499/j.issn.1008-8830.2015.03.011

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Chinese Journal of Contemporary Pediatrics ›› 2015, Vol. 17 ›› Issue (3) : 254-257. DOI: 10.7499/j.issn.1008-8830.2015.03.011

CLINICAL RESEARCH

Plasma proteomic analysis in children with infectious mononucleosis

RAN Zhi-Ling¹, XIAO Bin², LIU Hong-Rui¹, LIU You-Ping², SHENG Qiao-Ni¹

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Abstract

Objective To explore the abnormal expression of plasma proteins by analysis of proteomic expression profile in children with infectious mononucleosis (IM). Methods Two dimensional gel electrophoresis (2-DE) followed by the mass spectrometry was used to examine important protein spots with different expression levels between children with IM and normal controls. Results Seven differential proteins were obtained: hemopexin, vitamin D binding protein, fetuin A, C-reactive protein, apolipoprotein A, haptoglobin and transthyretin. Compared with the control group, haptoglobin showed a higher expression level in children with IM, and the expression levels of the other proteins were obviously down-regulated. Conclusions The expression changes of differential proteins identified in this study are all related with the liver acute injury, suggesting that children with IM are associated with acute liver injury. Further studies on the characteristics of above proteins will contribute to the diagnosis and treatment of pediatric IM.

Key words

Infectious mononucleosis / Plasma protein / Proteomics / Child

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RAN Zhi-Ling, XIAO Bin, LIU Hong-Rui, LIU You-Ping, SHENG Qiao-Ni. Plasma proteomic analysis in children with infectious mononucleosis[J]. Chinese Journal of Contemporary Pediatrics. 2015, 17(3): 254-257 https://doi.org/10.7499/j.issn.1008-8830.2015.03.011

References

[1] 李中跃, 楼金玕, 陈洁. 儿童EB 病毒感染首发症状及相关疾病谱分析[J]. 中华儿科杂志, 2004, 42(1): 20-22.
[2] 段红梅, 姚瑶, 谢正德, 等. 91 例EB 病毒相关疾病儿童血浆EB 病毒DNA 的检测[J]. 中国当代儿科杂志, 2009, 11(12): 897-900.
[3] 罗晓明, 周馥英, 周永列, 等. 儿童传染性单核细胞增多症B 淋巴细胞及其活化状况的研究[J]. 中华儿科杂志, 2004, 42(9): 701-704.
[4] 胡亚美, 江载芳, 诸福棠. 实用儿科学[M]. 第7 版. 北京: 人民卫生出版社, 2005: 824.
[5] 何大澄( 主译). 蛋白质与蛋白质组学实验指南(理查德J. 辛普森主编) [M]. 北京: 化学工业出版社, 2006: 134-150.
[6] 魏嵋, 刘友平, 郭芳宏, 等. 慢性乙型病毒性肝炎湿热中阻证血浆蛋白质组学分析[J]. 中国中西医结合杂志, 2011, 31(10): 1341-1345.
[7] Rolla S, Ingoglia G, Bardina V, et al. Acute-phase protein hemopexin is a negative regulator of Th17 response and experimental autoimmune encephalomyelitis development[J]. J Immunol, 2013, 191(11): 5451-5459.
[8] Larsen R, Gozzelino R, Jeney V, et al. A central role for free heme in the pathogenesis of severe sepsis[J]. Sci Transl Med, 2010, 2(51): 51-71.
[9] Lin T, Sammy F, Yang H, et al. Identification of hemopexin as an anti-inflammatory factor that inhibits synergy of hemoglobin with HMGB1 in sterile and infectious inflammation[J]. J Immunol, 2012, 189(4): 2017-2022.
[10] Speeckaert MM, Speeckaert R, van Geel N, et al. Vitamin D binding protein: a multifunctional protein of clinical importance[J]. Adv Clin Chem, 2014, 63: 1-57.
[11] Dziegielewska KM, Brown WM, Gould CC, et al. Fetuin: an acute phase protein in cattle[J]. J Comp Physiol B, 1992, 162(2): 168- 171.
[12] Kang WC, Ahn TH, Moon CI, et al. Impact of arterial remodeling on high sensitive C-reactive protein after a DES implantation[J]. Ant J Cardiol, 2010, 145(2): 325-326.
[13] van de Veerdonk FL, Wever PC, Hermans MH, et al. IL-18 serum concentration is markedly elevated in acute EBV infection and can serve as a marker for disease severity[J]. J Infect Dis, 2012, 206(2): 197-201.
[14] 何凯茵, 肖光明, 张春兰. 慢性乙型肝炎患者血脂和载脂蛋白水平变化及其意义[J]. 实用肝脏病杂志, 2005, 8(6): 326-327.
[15] Kaysen GA. Malnutrition and the acute-phase reaction in dialysis patients-how to measure and how to distinguish[J]. Nephrol Dial Transplant, 2000, 15(10): 1521-1524.
[16] Sarvari J, Mojtahedi Z, Kuramitsu Y, et al. Differential expression of haptoglobin isoforms in chronic active hepatitis, cirrhosis and HCC related to HBV infection[J]. Oncol Lett, 2011, 2(5): 871-877.
[17] Borlak J, Chatterji B, Londhe KB, et al. Serum acute phase reactants hallmark healthy individuals at risk for acetaminopheninduced liver injury[J]. Genome Med, 2013, 5(9): 86-99.
[18] Yao Y, Duan HM, Yan HM, et a1. Clinically controlled study on children,S infectious mononucleosis treated by Chinese medicine[J]. Chin J Integr Med, 2009, 15(5): 347-352.