Effect of L-alanyl-L-glutamine on expression of insulin-like growth factor-1 in intestinal tissues of low-birth-weight newborn rats with hypoxia/reoxygenation-induced intestinal injury

XU Fen; ZHU Chuan-Rui; ZHAN Yuan-Li; LU Guang-Jin; SU Hao-Bin

doi:10.7499/j.issn.1008-8830.2015.05.018

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Chinese Journal of Contemporary Pediatrics ›› 2015, Vol. 17 ›› Issue (5) : 502-507. DOI: 10.7499/j.issn.1008-8830.2015.05.018

EXPERIMENTAL RESEARCH

Effect of L-alanyl-L-glutamine on expression of insulin-like growth factor-1 in intestinal tissues of low-birth-weight newborn rats with hypoxia/reoxygenation-induced intestinal injury

XU Fen¹, ZHU Chuan-Rui¹, ZHAN Yuan-Li¹, LU Guang-Jin¹, SU Hao-Bin²

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Abstract

Objective To study the effect of L-alanyl-L-glutamine (Ala-Gln) on the levels of insulin-like growth factor-1 (IGF-1) and IGF-1 receptor (IGF-1R) in the intestinal tissues of low-birth-weight (LBW) newborn rats with hypoxia/reoxygenation-induced intestinal injury. Methods Pregnant rats were fed with or without smoking. The rats born by those fed without smoking were included in group A; for the rats born by those fed with smoking, normal-birth-weight rats were included in group B, and LBW rats were randomly divided into control group (group C), hypoxia/reoxygenation (H/R) group (group D), and Ala-Gln group (group E). Each group consisted of 24 newborn rats. The rats in groups D and E received H/R treatment twice a day for three consecutive days to establish an intestinal injury model; the rats in group E were intraperitoneally injected with Ala-Gln (10 ml/kg) before daily H/R treatment, while those in groups C and D were given an equal dose of normal saline by intraperitoneal injections. On days 4, 7, and 10 after birth, 8 rats were sacrificed in each group to collect intestinal tissues. The IGF-1 levels in intestinal tissues were measured using ELISA, and IGF-1R levels were measured by immunohistochemistry. Results There were no significant differences in IGF-1 and IGF-1R levels between groups A and B at all time points. The levels of IGF-1 and IGF-1R in group C kept increasing, were higher than those in other groups on day 7 (P<0.05), and reached a normal level on day 10, without significant differences compared with those in groups A and B. Group D had significantly lower IGF-1 and IGF-1R levels than group C at all time points (P<0.05). The levels of IGF-1 and IGF-1R in group E were lower than those in group C on days 4 and 7 (P<0.05), but they increased to approximately the levels in group C and were significantly higher than those in group D on day 10. Conclusions Intrauterine and postnatal hypoxia may induce intestinal injury in LBW newborn rats, and parenteral administration of high-dose Ala-Gln can reduce hypoxia-induced intestinal injury. Therefore, Ala-Gln has a protective effect against hypoxia-induced intestinal injury.

Key words

L-alanyl-L-glutamine / Low-birth-weight / Insulin-like growth factor-1 / Intestinal injury / Newborn rats

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XU Fen, ZHU Chuan-Rui, ZHAN Yuan-Li, LU Guang-Jin, SU Hao-Bin. Effect of L-alanyl-L-glutamine on expression of insulin-like growth factor-1 in intestinal tissues of low-birth-weight newborn rats with hypoxia/reoxygenation-induced intestinal injury[J]. Chinese Journal of Contemporary Pediatrics. 2015, 17(5): 502-507 https://doi.org/10.7499/j.issn.1008-8830.2015.05.018

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