Abstract Objective To explore the effect of non-methylated cytosine-phosphate-guanine oligodeoxynucleotides (CpG-ODN) on serum transforming growth factor (TGF)-β and immune regulation in ovalbumin (OVA)-sensitized young mice. Methods Thirty female BALB/c mice (2-3 weeks old) were randomly divided into control, model, and CpG-ODN intervention groups. A young mouse model of food allergy was established by OVA sensitization. Normal saline of the same volume was used for replacement in the control group. The mice in the intervention group were intraperitoneally injected with CpG-ODN solution 1 hour before every OVA sensitization. Allergic symptoms were observed and scored for each group. The jejunal tissue was histopathologically examined with hematoxylin-eosin staining. Serum OVA-IgE level was measured using ELISA. Serum concentrations of interleukin (IL)-4, interferon (IFN)-γ, and TGF-β were determined by CBA. Results Allergic symptoms were observed in the model group and the jejunal tissue showed the pathological characteristics of type I allergic reaction. The allergic symptom scores in the model and CpG-ODN intervention groups were significantly higher than in the control group (P< 0.01). The serum levels of OVA-IgE, IL-4, and TGF-β were significantly higher in the model group than in the control and CpG-ODN intervention groups (P< 0.05). The CpG-ODN intervention group had significantly higher serum levels of OVA-IgE, IL-4, and TGF-β than the control group (P< 0.05). Compared with the control and CpG-ODN intervention groups, the model group had a significantly reduced IFN-γ level (P< 0.05). Conclusions The serum TGF-β level is increased in the young mouse model of OVA-sensitized food allergy and is involved in the allergy mechanism. Non-methylated CpG-ODN can reduce the serum TGF-β level in sensitized young mice and play an immunoregulatory role in food allergy.
About author:: 10.7499/j.issn.1008-8830.2015.08.020
Cite this article:
WANG Ben-Zhen,ZHENG Cheng-Zhong. Effect of non-methylated CpG-ODN on serum TGF-β and immune regulation in ovalbumin-sensitized young mice[J]. CJCP, 2015, 17(8): 864-868.
WANG Ben-Zhen,ZHENG Cheng-Zhong. Effect of non-methylated CpG-ODN on serum TGF-β and immune regulation in ovalbumin-sensitized young mice[J]. CJCP, 2015, 17(8): 864-868.
Li F, Wang L, Jin XM, et al. The immunologic effect of TGF-beta1 chitosannanoparticle plasmids on ovalbumin-induced allergic BALB/c mice[J]. Immunobiology, 2009, 214(2): 87-99.
[2]
Pali-Schöll I, Szöllösi H, Starkl P, et al. Protamine nanoparticles with CPG-oligodeoxynucleotide prevent an allergen-induced Th2-response in BALB/c mice[J]. Eur J Pharm Biopharm, 2013, 85(3 Pt A): 656-664.
[3]
Oyoshi MK, Oettgen HC, Chatila TA, et al. Food allergy: Insights into etiology, prevention, and treatment provided by murine models[J]. J Allergy Clin Immunol, 2014, 133(2): 309-317.
[4]
Shin HS, Bae MJ, Jung SY, et al. Preventive effects of skullcap (Scutellaria baicalensis) extract in a mouse model of food allergy[J]. J Ethnopharmacol, 2014, 153(3): 667-673.
[5]
Knippels LM, Penninks AH, Spanhaak S, et al. Oral sensitization to food proteins: a brown norway rat model[J]. Clin Exp Allergy, 1998, 28(3): 368-375.
[6]
Li XM, Schofield BH, Huang CK, et al. A murine model of IgE-mediated cow's milk hypersensitivity[J]. J Allergy Clin Immunol, 1999, 103(2 Pt 1): 206-214.
Ben-Shoshan M, Clarke AE. Anaphylaxis: past, present and future[J]. Allergy, 2011, 66(1): 1-14.
[9]
Toomer OT, Ferguson M, Pereira M, et al. Maternal and postnatal dietary probiotic supplementation enhances splenic regulatory T helper cell population and reduces ovalbumin allergen-induced hypersensitivity responses in mice[J]. Immunobiology, 2014, 219(5): 367-376.
[10]
Lin YT, Wu CT, Huang JL, et al. Correlation of ovalbumin of egg white components with allergic diseases in children[J]. J Microbiol Immunol Infect, 2014: S1684-S1182.
[11]
Chen C, Sun NN, Li YN, et al. A BALB/c mouse model for assessing the potential allergenicity of proteins: comparison of allergen dose, sensitization frequency,timepoint and sex[J]. Food Chem Toxicol, 2013, 62(62): 41-47.
Sakamoto Y, Ueno K, Yofu S, et al. The expression of IL-4, IL-6 and TNF-a in food sensitized mice after oral challenge[J]. Int Arch Allergy Immunol, 1999, 118(4): 226.
Palomares O, Martin-fontecha M, Lauener R, et al. Regulatory T cell and immune regulation of allergic diseases: roles of IL-10 and TGF-β[J]. Genes Immun, 2014, 24: 1-10.
[16]
Midence NG, Arias K. TGF-βregulates house dust mite-induced allergic airway inflammation but not airway remodeling[J]. Am J Respir Crit Care Med, 2008, 177(6): 593-603.
[17]
Cardoso CR, Provinciatto PR, Godoi DF, et al. B cells are involved in the Modulation of pathogenic gut immune response in food-allergic entropathy[J]. Clin ExpImmunol, 2008, 154(2): 153-161.
[18]
Jassies-van der Lee A, Rutten V, Spiering R, et al. The immunostimulatory effect of CpG oligodeoxynucleotides on peripheral blood mononuclear cells of healthy dogs and dogs with atopic dermatitis[J]. Vet J, 2014, 200(1): 103-108.
[19]
Asanuma H, Zamri NB, Sekine S, et al. A novel combined adjuvant for nasal delivery elicits mucosal immunity to influenza in aging[J]. Vaccine, 2012, 30(4): 803-812.
[20]
Cheng Q, Xu C, Zhang L, et al. Administered CpG oligodeoxynucleotide induces mRNA expression of CXC and CC chemokine s at the intestinal mucosa and PBMCs in piglets[J]. Int Immunopharmacol, 2010, 10(5): 611-618.
[21]
Nishida A, Lau C, Mizoguchi E, et al. Regulatory B cells in mouse models of intestinal inflammation[J]. Methods Mol Biol, 2014, 1190: 227-241.
[22]
Cuschieri J, Maier RV. Mitogen-activated protein kinase (MAPK)[J]. Crit Care Med, 2005, 33(12 Suppl): S417-S419.
[23]
Dupont J, McNeilly J, Vaiman A, et a1. Activin signaling pathways in ovine pituitary and LbetaT2 gonadotrope cells[J]. Biol Reprod, 2003, 68(5): 1877-1887.
[24]
Volpi C, Fallarino F, Pallotta MT, et al. High doses of CpG oligodeoxynucleotide s stimulate a tolerogenic TLR9-TRIF pathway[J]. Nat Commun, 2013, 4: 1852.
