Abstract Objective To compare the detection rates of Mycoplasma pneumoniae (MP) from nasopharyngeal aspirates (NPA) and bronchoalveolar lavage fluid (BALF) in children with pneumonia.Methods A total of 164 hospitalized children with pneumonia were enrolled. NPA and BALF of these children were collected within 24 hours of admission, and MP-DNA was detected by fluorescence quantitative PCR. Venous blood samples of all these children were collected within 24 hours of admission and on days 7-10 of treatment, and serum MP-IgM was detected using ELISA.Results The positive rate of MP-DNA in NAP of the 164 cases was 51.8%, which was lower than 63.4% as the detection rate of MP-IgM in serum (P=0.044), and the two detection rates were moderately consistent with each other (Kappa=0.618, PP>0.05), and the detection rates were well consistent (Kappa=0.793, PPKappa=0.529, PPPPConclusions MP-DNA in BALF has a high sensitivity, with a great significance for early diagnosis of MP pneumonia, while NPA MP-DNA tests may lead to a missed diagnosis.
GU Wen-Jing,ZHANG Xin-Xing,CHEN Zheng-Rong et al. Clinical significance of MP-DNA from endotracheal aspirates in diagnosis of Mycoplasma pneumoniae pneumonia in children[J]. CJCP, 2015, 17(9): 937-941.
GU Wen-Jing,ZHANG Xin-Xing,CHEN Zheng-Rong et al. Clinical significance of MP-DNA from endotracheal aspirates in diagnosis of Mycoplasma pneumoniae pneumonia in children[J]. CJCP, 2015, 17(9): 937-941.
Guo Q, Li HY, Zhou YP, et al. Associations of radiological features in Mycoplasma pneumoniae pneumonia[J]. Arch Med Sci, 2014, 10(4):725-732.
[8]
Liu FC, Chen PY, Huang F, et al. Rapid diagnosis of Mycoplasma pneumoniae infection in children by polymerase chain reaction[J]. J Microbiol Immunol Infect, 2007, 40(6):507-512.
Akihiro T, Kousaku M, Takayuki T, et al. Methylprednisolone pulse therapy for refractory mycoplasma pneumoniae pneumonia in children[J]. J Infect, 2008, 57(3):223-228.
[11]
Waites KB, Balish MF, Atkinson TP. New insights into the pathogenesis and detection of Mycoplasma pneumoniae infection[J]. Future Microbiol, 2008, 3(6):635-648.
Kashyap B, Kumar S, Sethi GR, et al. Comparison of PCR, culture & serological tests for the diagnosis of Mycoplasma pneumoniae in counity acquired lower respiratory tract infections in children[J]. Indian J Med Res, 2008, 128(2):134-139.
