Expression profiles of miRNA-182 and Clock mRNA in the pineal gland of neonatal rats with hypoxic-ischemic brain damage

HAN Xing; DING Xin; XU Li-Xiao; LIU Ming-Hua; FENG Xing

doi:10.7499/j.issn.1008-8830.2016.03.016

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Chinese Journal of Contemporary Pediatrics ›› 2016, Vol. 18 ›› Issue (3) : 270-276. DOI: 10.7499/j.issn.1008-8830.2016.03.016

EXPERIMENTAL RESEARCH

Expression profiles of miRNA-182 and Clock mRNA in the pineal gland of neonatal rats with hypoxic-ischemic brain damage

HAN Xing, DING Xin, XU Li-Xiao, LIU Ming-Hua, FENG Xing

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Abstract

Objective To study the changes of miRNA expression in the pineal gland of neonatal rats with hypoxic-ischemic brain damage (HIBD) and the possible roles of miRNA in the pathogenesis of circadian rhythm disturbance after HIBD. Methods Seven-day-old Sprague-Dawley (SD) rats were randomly divided into 2 groups: HIBD and sham-operated. HIBD was induced according to the Rice-Vannucci method. The pineal glands were obtained 24 hours after the HIBD event. The expression profiles of miRNAs were determined using GeneChip technigue and quantitative real-time PCR (RT-PCR). Then the miRNA which was highly expressed was selected. The expression levels of the chosen miRNA were detected in different tissues (lungs, intestines, stomach, kidneys, cerebral cortex, pineal gland). RT-PCR analysis was performed to measure the expression profiles of the chosen miRNA and the targeted gene Clock mRNA in the pineal gland at 0, 24, 48 and 72 hours after HIBD. Results miRNA-182 that met the criteria was selected by GeneChip and RT-PCR. miRNA-182 was highly expressed in the pineal gland. Compared with the shamoperated group, the expression of miRNA-182 was significantly up-regulated in the pineal gland at 24 and 48 hours after HIBD (P<0.05). Compared with the sham-operated group, Clock mRNA expression in the HIBD group increased at 0 hour after HIBD, decreased at 48 hours after HIBD and increased at 72 hours after HIBD (P<0.05). Conclusions miRNA-182 may be involved in the pathogenesis of circadian rhythm disturbance after HIBD.

Key words

miRNA / miRNA-182 / Hypoxic-ischemic brain damage / Neonatal rats

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HAN Xing, DING Xin, XU Li-Xiao, LIU Ming-Hua, FENG Xing. Expression profiles of miRNA-182 and Clock mRNA in the pineal gland of neonatal rats with hypoxic-ischemic brain damage[J]. Chinese Journal of Contemporary Pediatrics. 2016, 18(3): 270-276 https://doi.org/10.7499/j.issn.1008-8830.2016.03.016

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