Influence of cefuroxime sodium on synaptic plasticity of parallel fiber-Purkinje cells in young rats

HE Hai-Yan; REN Ying-Ge; LI Ling; JIN Fu-Li; DU Yong-Ping; ZHANG Yue-Ping

doi:10.7499/j.issn.1008-8830.2016.06.017

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Chinese Journal of Contemporary Pediatrics ›› 2016, Vol. 18 ›› Issue (6) : 558-563. DOI: 10.7499/j.issn.1008-8830.2016.06.017

EXPERIMENTAL RESEARCH

Influence of cefuroxime sodium on synaptic plasticity of parallel fiber-Purkinje cells in young rats

HE Hai-Yan^1,2, REN Ying-Ge¹, LI Ling¹, JIN Fu-Li¹, DU Yong-Ping¹, ZHANG Yue-Ping¹

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Abstract

Objective To investigate the influence of cefuroxime sodium (CS) on the electrophysiological function of cerebellar Purkinje cells (PCs) in Sprague-Dawley rats. Methods Postnatal day 7 (P7) Sprague-Dawley rats were divided into early administration I and II groups (administered from P7 to P14) and late administration group (administered from P14 to P21), and all the groups received intraperitoneally injected CS. The control groups for early and late administration groups were also established and treated with intraperitoneally injected normal saline of the same volume. There were 10 rats in each group. The rats in the early administration I group and early administration control group were sacrificed on P15, and those in the early administration II group, late administration group, and late administration control group were sacrificed on P22. The whole-cell patch-clamp technique was used to record inward current and action potential of PCs on cerebellar slices, as well as the long-term depression (LTD) of excitatory postsynaptic current (EPSC) in PCs induced by low-frequency stimulation of parallel fiber (PF). Results Compared with the control groups, the early and late administration groups had a slightly higher magnitude of inward current and a slightly higher amplitude of action potential of PCs (P > 0.05). All administration groups had a significantly higher degree of EPSC inhibition than the control groups (P < 0.01), and the early administration II group had a significantly greater degree of EPSC inhibition than the late administration group (P < 0.01). Conclusions Early CS exposure after birth affects the synaptic plasticity of PF-PCs in the cerebellum of young rats, which persists after drug withdrawal.

Key words

Cefuroxime sodium / Cerebellum / Purkinje cell / Synaptic plasticity / Young rats

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HE Hai-Yan, REN Ying-Ge, LI Ling, JIN Fu-Li, DU Yong-Ping, ZHANG Yue-Ping. Influence of cefuroxime sodium on synaptic plasticity of parallel fiber-Purkinje cells in young rats[J]. Chinese Journal of Contemporary Pediatrics. 2016, 18(6): 558-563 https://doi.org/10.7499/j.issn.1008-8830.2016.06.017

References

[1] Schulman J, Dimand RJ, Lee HC, et al. Neonatal intensive care unit antibiotic use[J]. Pediatrics, 2015, 135(5): 826-833.
[2] 贺瑞萍, 郭永英. 抗生素类药物对神经系统的毒性反应[J]. 中国药事, 2004, 18(12): 774-775.
[3] Bercik P, Denou E, Collins J, et al. The intestinal microbiota affect central levels of brain-derived neurotropic factor and behavior in mice[J]. Gastroenterology, 2011, 141(2): 599-609.
[4] House SA, Goodman DC, Weinstein SJ, et al. Prescription use among children with autism spectrum disorders in Northern New England: intensity and small area variation[J]. J Pediatr, 2016, 169: 277-283.e2.
[5] de Louvois J, Mulhall A, Hurley R. Cefuroxime in the treatment of neonates[J]. Arch Dis Child, 1982(1), 57: 59-62.
[6] Riedel MC, Ray KL, Dick AS, et al. Meta-analytic connectivity and behavioral parcellation of the human cerebellum[J]. NeuroImage, 2015, 117: 327-342.
[7] Zhang Y, Magnus G, Han VZ. Synaptic dynamics and long-time plasticity at synapses of Purkinje cells onto neighboring Purkinje cells of a mormyrid fish: A dual cell recording study[J]. Neuroscience, 2012, 225: 199-212.
[8] 孙瑞元, 马越鸣, 洪宗元. 药理实验设计中的剂量问题[M]// 徐叔云, 卞如濂, 陈修. 药理实验方法学. 第3版. 北京: 人民卫生出版社, 2002: 202-204.
[9] 李芳梅, 曹凤美, 晁兰芳. 抗生素对神经和心肺的副作用 [J]. 医学理论与实践, 2001, 14(12): 1228-1229.
[10] Kim CH, Oh SH, Lee JH, et al. Lobule-specific membrane excitability of cerebellar Purkinje cells[J]. J Physiol, 2012, 590(Pt2): 273-288.
[11] Masoli S, Solinas S, D'Angelo E. Action potential processing in a detailed Purkinje cell model reveals a critical role for axonal compartmentalization[J]. Front Cell Neurosci, 2015, 9: 1-22.
[12] 邓敏, 王云甫, 胡芳, 等. 不同种类抗生素影响重症肌无力小鼠神经肌肉接头处的传递功能[J]. 中国临床康复, 2005, 9(17): 233-235.
[13] Johnston MV, Ishida A, Ishida WN, et al. Plasticity and injury in the developing brain[J]. Brain Dev, 2009, 31(1): 1-10.
[14] Cohen-Cory S. The developing synapse: construction and modulation of synaptic structures and circuits[J]. Science, 2002, 298(5594): 770-776.
[15] Schonewille M, Gao Z, Boele HJ, et al. Reevaluating the role of LTD in cerebellar motor learning[J]. Neuron, 2011, 70(1): 43-50.
[16] Gao Z, van Beugen BJ, De Zeeuw CI, et al. Distributed synergistic plasticity and cerebellar learning[J]. Nat Rev Neurosci, 2012, 13(9): 619-635.
[17] Ito M, Yamaguchi K, Nagao S, et al. Long-term depression as a model of cerebellar plasticity[J]. Prog Brain Res, 2014, 210: 1-30.
[18] Mapelli L, Pagani M, Garrido JA, et al. Integrated plasticity at inhibitory and excitatory synapses in the cerebellar circuit[J]. Front Cell Neursci, 2015, 9: 169.
[19] Linden DJ, Dickinson MH, Smeyne M, et al. A long-term depression of AMPA currents in cultured cerebellar Purkinje neurons[J]. Neuron, 1991, 7(1): 81-89.
[20] Kohda K, Kakegawa W, Yuzaki M. Unlocking the secrets of the δ2 glutamate receptor: A gatekeeper for synaptic plasticity in the cerebellum[J]. Commun Integr Biol, 2013, 6(6): e26466.
[21] D'Angelo E. The organization of plasticity in the cerebellar cortex: from synapses to control[M]//Ramnani N. Prog Brain Res(Vol.210). Amsterda: Elsevier Science BV, 2014: 31-58.
[22] Zhao LS, Yin R, Wei BB, et al. Comparative pharmacokinetics of cefuroxime lysine after single intravenous, intraperitoneal, and intramuscular administration to rats[J]. Acta Pharmacol Sin, 2012, 33(11): 1348-1352.
[23] 安备, 杜湘珂, 霍天龙,等. SD大鼠腹腔注射及尾静脉注射18F-FDG PET/CT成像对比研究[J]. 医学研究杂志, 2013, 42(5): 48-52.
[24] Soll RF, Edwards WH. Antibiotic use in neonatal intensive care[J]. Pediatrics, 2015, 135(5): 928-929.
[25] 关小萍, 梁春华, 陈延欢. 新生儿肺炎疗效及肠道微生态的研究 [J]. 中国实用医药, 2009, 4(21): 15-17.
[26] 王凤科. 广谱抗生素对感染性疾病患者肠道微生态的影响[J]. 吉林医学, 2010, 31(32): 5726-5728.
[27] 栾娜, 孙运波, 段峰. 头孢曲松钠对菌血症小鼠肠道膜菌群的影响[J]. 齐鲁医学杂志, 2007, 22(4): 326-328.