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Effect of rhubarb on neonatal rats with bronchopulmonary dysplasia induced by hyperoxia
YIN Ling-Ling, YE Zhen-Zhi, TANG Li-Jun, GUO Liang, HUANG Wei-Min
Chinese Journal of Contemporary Pediatrics ›› 2018, Vol. 20 ›› Issue (5) : 410-415.
PDF(1756 KB)
PDF(1756 KB)
Effect of rhubarb on neonatal rats with bronchopulmonary dysplasia induced by hyperoxia
Objective To study the effect of rhubarb on neonatal rats with bronchopulmonary dysplasia (BPD) induced by hyperoxia. Methods A total of 64 rats (postnatal day 4) were randomly divided into four groups:air control, rhubarb control, hyperoxia model, and hyperoxia+rhubarb (n=16 each). The rats in the hyperoxia model and hyperoxia+rhubarb groups were exposed to hyperoxia (60% O2) to establish a BPD model. The rats in the rhubarb control and hyperoxia+rhubarb groups were given rhubarb extract suspension (600 mg/kg) by gavage daily. The pathological changes of lung tissue were evaluated by hematoxylin-eosin staining on postnatal days 14 and 21. The content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) were measured by spectrophotometry. The mRNA and protein expression levels of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were determined by RT-PCR and Western blot respectively. Results The hyperoxia model group showed reduced alveolar number, increased alveolar volume, and simplified alveolar structure, which worsened over the time of exposure to hyperoxia. These pathological changes were significantly reduced in the hyperoxia+rhubarb group. On postnatal days 14 and 21, compared with the air control and rhubarb control groups, the hyperoxia model group had significantly reduced radical alveolar count (RAC), significantly reduced activity of SOD in the lung tissue, and significantly increased content of MDA and mRNA and protein expression levels of TNF-α and IL-6 (P < 0.05). Compared with the hyperoxia model group, the hyperoxia+rhubarb group had significantly increased RAC, significantly increased activity of SOD in the lung tissue, and significantly reduced content of MDA and mRNA and protein expression levels of TNF-α and IL-6 (P < 0.05). Conclusions Rhubarb may play a protective role in rats with BPD induced by hyperoxia through inhibiting inflammatory response and oxidative stress.
Bronchopulmonary dysplasia / Rhubarb / Inflammation / Oxidative stress / Rats
[1] Poggi C, Dani C. Antioxidant strategies and respiratory disease of the preterm newborn:an update[J]. Oxid Med Cell Longev, 2014, 2014:721043.
[2] Shahzad T, Radajewski S, Chao CM, et al. Pathogenesis of bronchopulmonary dysplasia:when inflammation meets organ development[J]. Mol Cell Pediatr, 2016, 3(1):23.
[3] Husari A, Khayat A, Bitar H, et al. Antioxidant activity of pomegranate juice reduces acute lung injury secondary to hyperoxia in an animal model[J]. BMC Res Notes, 2014, 7:664.
[4] Balany J, Bhandari V. Understanding the impact of infection, inflammation, and their persistence in the pathogenesis of bronchopulmonary dysplasia[J]. Front Med (Lausanne), 2015, 2:90.
[5] 郭丽纳, 孙燕妮. 大黄素对急性肺损伤保护作用研究进展[J]. 中国中医急症, 2017, 26(4):663-665.
[6] Xiao M, Zhu T, Zhang W, et al. Emodin ameliorates LPSinduced acute lung injury, involving the inactivation of NF-κB in mice[J]. Int J Mol Sci, 2014, 15(11):19355-19368.
[7] 龙勇, 姜英, 李长罗, 等. 大黄对百草枯中毒大鼠肺损伤的保护作用[J]. 中国中医急症, 2016, 25(9):1734-1736.
[8] 黄铃沂, 段江, 封志纯. 支气管肺发育不良小鼠模型的建立[J]. 中国实验动物学报, 2009, 17(3):186-188.
[9] Han W, Guo C, Liu Q, et al. Aberrant elastin remodeling in the lungs of O2-exposed newborn mice; primarily results from perturbed interaction between integrins and elastin[J]. Cell Tissue Res, 2015, 359(2):589-603.
[10] 崔换金, 黄为民, 何嘉裕. 高氧暴露新生大鼠肺组织转录因子CASZ1的表达及其与肺微血管发育的关系[J]. 中华儿科杂志, 2016, 54(1):37-42.
[11] Merritt T A,Deming D D,Boynton B R.The ‘new’ bronchopulmonary dysplasia:challenges and commentary[J]. Semin Fetal Neonatal Med, 2009, 14(6):345-357.
[12] 王艳, 江来. 红景天苷对机械通气致小鼠急性肺损伤的保护作用研究[J]. 国际麻醉学与复苏杂志, 2017, 38(3):193-197.
[13] Xu W, Zhao Y, Zhang B, et al. Resveratrol attenuates hyperoxiainduced oxidative stress, inflammation and fibrosis and suppresses Wnt/β-catenin signalling in lungs of neonatal rats[J]. Clin Exp Pharmacol Physiol, 2015, 42(10):1075-1083.
[14] Bhandari V. Postnatal inflammation in the pathogenesis of bronchopulmonary dysplasia[J]. Birth Defects Res A Clin Mol Teratol, 2014, 100(3):189-201.
[15] Hsiao CC, Chang JC, Tsao LY, et al. Correlates of elevated interleukin-6 and 8-hydroxy-2'-deoxyguanosine levels in tracheal aspirates from very low birth weight infants who develop bronchopulmonary dysplasia[J]. Pediatr Neonatol, 2017, 58(1):63-69.
[16] Choi CW, Kim BI, Kim HS, et al. Increase of interleukin-6 in tracheal aspirate at birth:a predictor of subsequent bronchopulmonary dysplasia in preterm infants[J]. Acta Paediatr, 2006, 95(1):38-43.
[17] Lai F, Zhang Y, Xie DP, et al. A systematic review of rhubarb (a traditional chinese medicine) used for the treatment of experimental sepsis[J]. Evid Based Complement Alternat Med, 2015, 2015:131283.
