Abstract Objective To study the expression of interferon-λ1 (IFN-λ1) in respiratory epithelial cells in children with human rhinovirus (HRV) infection. Methods Sputum samples and nasopharyngeal swabs were collected from the children who were hospitalized due to acute respiratory infection from February to October, 2017. Bacterial culture was performed, and nucleic acid test was performed for 11 respiratory pathogens. A total of 90 children with positive HRV alone were enrolled as the HRV infection group, and 95 children with positive respiratory syncytial virus (RSV) alone were enrolled as the RSV infection group. A total of 50 healthy children who underwent outpatient physical examination during the same period of time and had negative results for all pathogen tests were enrolled as the healthy control group. Nasopharyngeal swabs were collected from all groups, and quantitative real-time PCR was used to measure viral load and the mRNA expression of IFN-λ1. Results In the HRV infection group, there was no significant difference in the mRNA expression of IFN-λ1 between boys and girls and across all age groups (P > 0.05). In the HRV infection group, there was no correlation between the mRNA expression of IFN-λ1 and HRV load (P > 0.05). The mRNA expression of IFN-λ1 in the HRV infection group was significantly higher than that in the healthy control group (P < 0.05), but significantly lower than that in the RSV infection group (P < 0.05). Conclusions HRV can induce the expression of IFN-λ1 in respiratory epithelial cells, suggesting that IFN-λ1 may play an important role in anti-HRV infection in children.
LIN Xiao-Juan,ZHONG Li-Li,XIE Ya-Ping et al. Expression of interferon-λ1 in respiratory epithelial cells in children with human rhinovirus infection[J]. CJCP, 2019, 21(12): 1177-1181.
LIN Xiao-Juan,ZHONG Li-Li,XIE Ya-Ping et al. Expression of interferon-λ1 in respiratory epithelial cells in children with human rhinovirus infection[J]. CJCP, 2019, 21(12): 1177-1181.
Minor DM, Proud D. Role of human rhinovirus in triggering human airway epithelial-mesenchymal transition[J]. Respir Res, 2017, 18(1):110.
[2]
Lasfar A, Zloza A, de la Torre A, et al. IFN-λ:a new inducer of local immunity against cancer and infections[J]. Front Immunol, 2016, 7:598.
[3]
Lauber C, Vieyres G, Terczyńska-Dyla E, et al. Transcriptome analysis reveals a classical interferon signature induced by IFNλ4 in human primary cells[J]. Genes Immun, 2015, 16(6):414-421.
[4]
Randall RE, Goodbourn S. Interferons and viruses:an interplay between induction, signalling, antiviral responses and virus countermeasures[J]. J Gen Virol, 2008, 89(Pt 1):1-47.
[5]
Selvaggi C, Pierangeli A, Fabiani M, et al. Interferon lambda 1-3 expression in infants hospitalize for RSV or HRV associated bronchiolitis[J]. J Infect, 2014, 68(5):467-477.
Sheppard P, Kindsvogel W, Xu W, et al. IL-28, IL-29 and their class II cytokine receptor IL-28R[J]. Nat Immunol, 2003, 4(1):63-68.
[8]
Contoli M, Message SD, Laza-Stanca V, et al. Role of deficient type III interferon-lambda production in asthma exacerbations[J]. Nat Med, 2006, 12(9):1023-1026.
[9]
Baraldo S, Contoli M, Bazzan E, et al. Deficient antiviral immune responses in childhood:distinct roles of atopy and asthma[J]. J Allergy Clin Immunol, 2012, 130(6):1307-1314.
[10]
Spann KM, Baturcam E, Schagen J, et al. Viral and host factors determine innate immune responses in airway epithelial cells from children with wheeze and atopy[J]. Thorax, 2014, 69(10):918-925.
Miller EK, Hernandez JZ, Wimmenauer V, et al. A mechanistic role for type III IFN-λ1 in asthma exacerbations mediated by human rhinoviruses[J]. Am J Respir Crit Care Med, 2012, 185(5):508-516.
[13]
Scagnolari C, Midulla F, Trombetti S, et al. Upregulation of interferon-induced genes in infants with virus-associated acute bronchiolitis[J]. Exp Biol Med (Maywood), 2007, 232(10):1355-1359.
[14]
Jewell NA, Cline T, Mertz SE, et al. Lambda interferon is the predominant interferon induced by influenza A virus infection in vivo[J]. J Virol, 2010, 84(21):11515-11522.
[15]
Ahn JG, Kim DS, Kim KH. Clinical characteristics and cytokine profiles of children acute lower respiratory tract infections caused by human rhinovirus[J]. PLoS One, 2018, 13(7):e0198624.
[16]
van Elden LJ, Sachs AP, van Loon AM, et al. Enhanced severity of virus associated lower respiratory tract disease in asthma patients may not be associated with delayed viral clearance and increased viral load in the upper respiratory tract[J]. J Clin Virol, 2008, 41(2):116-121.
[17]
Pierangeli A, Statzu M, Nenna R, et al. Interferon lambda receptor 1(IFNL1R) transcript is highly expressed in rhinovirus bronchiolitis and correlates with disease severity[J]. J Clin Virol, 2018, 102:101-109.
[18]
Jordan WJ, Eskdale J, Boniotto M, et al. Modulation of the human cytokine response by interferon lambda-1(IFN-lambda1/IL-29)[J]. Genes Immun, 2007, 8(1):13-20.
[19]
Pekarek V, Srinivas S, Eskdale J. Interferon lambda-1(IFN-lambda1/IL-29) induces ELR(-) CXC chemokine mRNA in human peripheral blood mononuclear cells, in an IFN-gamma- independent manner[J]. Genes Immun, 2007, 8(2):177-180.