不同肠内营养开始时间对极低出生体重儿肠道菌群及代谢产物影响的前瞻性研究

doi:10.7499/j.issn.1008-8830.2111165

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Abstract Objective To study the influence of enteral feeding initiation time on intestinal flora and metabolites in very low birth weight (VLBW) infants. Methods A total of 29 VLBW infants who were admitted to the Department of Neonatology, Children's Hospital of Chongqing Medical University, from June to December, 2020, were enrolled as subjects. According to the enteral feeding initiation time after birth, the infants were divided into two groups: <24 hours (n=15) and 24-72 hours (n=14). Fecal samples were collected at weeks 2 and 4 of hospitalization, and 16S rDNA high-throughput sequencing and gas chromatography-mass spectrometry were used to analyze the microflora and short-chain fatty acids (SCFAs) respectively in fecal samples. Results The analysis of microflora showed that there was no significant difference between the two groups in Chao index (reflecting the abundance of microflora) and Shannon index (reflecting the diversity of microflora) at weeks 2 and 4 after birth (P>0.05). The analysis of flora composition showed that there was no significant difference in the main microflora at the phylum and genus levels between the two groups at weeks 2 and 4 after birth (P>0.05). The comparison of SCFAs between the two groups showed that the <24 hours group had a significantly higher level of propionic acid than the 24-72 hours group at week 4 (P<0.05), while there was no significant difference in the total amount of SCFAs and the content of the other SCFAs between the two groups (P>0.05). Conclusions Early enteral feeding has no influence on the diversity and abundance of intestinal flora in VLBW infants, but enteral feeding within 24 hours can increase the level of propionic acid, a metabolite of intestinal flora.

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	Articles by authors
	SHEN Yu-Jie
	LI Lu-Quan
	WEI Lu
	ZHANG Xian-Hong
	ZHAO Wen-Jing
	LIU Xiao-Chen
	WU Li-Ping

Key words： Enteral feeding Initiation time Intestinal flora Short-chain fatty acid Very low birth weight infant

Received: 26 November 2021

Cite this article:

SHEN Yu-Jie,LI Lu-Quan,WEI Lu et al. Influence of enteral feeding initiation time on intestinal flora and metabolites in very low birth weight infants: a prospective study[J]. CJCP, 2022, 24(4): 433-439.

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http://www.zgddek.com/EN/10.7499/j.issn.1008-8830.2111165 OR http://www.zgddek.com/EN/Y2022/V24/I4/433

	Chen YW, Zhou JH, Wang L. Role and mechanism of gut microbiota in human disease[J]. Front Cell Infect Microbiol, 2021, 11: 625913. PMID: 33816335. PMCID: PMC8010197. DOI: 10.3389/fcimb.2021.625913.
	2 Salas AA, Willis KA, Carlo WA, et al. The gut microbiome of extremely preterm infants randomized to the early progression of enteral feeding[J]. Pediatr Res, 2021. PMID: 34775476. DOI: 10.1038/s41390-021-01831-w. Epub ahead of print.
	3 魏涛, 彭思意, 李旭英, 等. 新生儿PICC相关感染影响因素的Meta分析[J]. 中国护理管理, 2019, 19(3): 380-387. DOI: 10.3969/j.issn.1672-1756.2019.03.012.
	4 丁国芳. 极低出生体重儿尽早达到足量肠内营养喂养策略——《极低出生体重儿喂养指南》解读[J]. 中国实用儿科杂志, 2016, 31(2): 85-89. DOI: 10.7504/ek2016020602.
	Tewari VV, Dubey SK, Kumar R, et al. Early versus late enteral feeding in preterm intrauterine growth restricted neonates with antenatal doppler abnormalities: an open-label randomized trial[J]. J Trop Pediatr, 2018, 64(1): 4-14. PMID: 28369652. DOI: 10.1093/tropej/fmx018.
	Nakubulwa C, Musiime V, Namiiro FB, et al. Delayed initiation of enteral feeds is associated with postnatal growth failure among preterm infants managed at a rural hospital in Uganda[J]. BMC Pediatr, 2020, 20(1): 86. PMID: 32093661. PMCID: PMC7038603. DOI: 10.1186/s12887-020-1986-5.
	中国医师协会新生儿科医师分会循证专业委员会. 早产儿喂养不耐受临床诊疗指南(2020)[J]. 中国当代儿科杂志, 2020, 22(10): 1047-1055. PMID: 33059799. PMCID: PMC7568993. DOI: 10.7499/j.issn.1008-8830.2008132.
	8 王丹华. 早产儿肠内营养新概念[J]. 临床儿科杂志, 2007, 25(3): 174-178. DOI: 10.3969/j.issn.1000-3606.2007.03.004.
	Ahmed F, Dey SK, Shahidullah M, et al. Early versus delayed enteral feeding for achieving full feeding in preterm growth-restricted infants: a randomized clinical trial[J]. Mymensingh Med J, 2020, 29(3): 638-645. PMID: 32844806.
	于新颖, 范玲. 肠内营养开始时间对极低出生体重儿消化功能及生长速度的影响[J]. 中国当代儿科杂志, 2014, 16(8): 814-819. PMID: 25140774. DOI: 10.7499/j.issn.1008-8830.2014.08.009.
	Gil A, Rueda R, Ozanne SE, et al. Is there evidence for bacterial transfer via the placenta and any role in the colonization of the infant gut?—a systematic review[J]. Crit Rev Microbiol, 2020, 46(5): 493-507. PMID: 32776793. DOI: 10.1080/1040841X.2020.1800587.
	Mitchell CM, Mazzoni C, Hogstrom L, et al. Delivery mode affects stability of early infant gut microbiota[J]. Cell Rep Med, 2020, 1(9): 100156. PMID: 33377127. PMCID: PMC7762768. DOI: 10.1016/j.xcrm.2020.100156.
	孙倩, 王政力, 刘晓晨, 等. 广谱抗生素疗程对极低出生体重儿粪便肠道菌群和短链脂肪酸影响的前瞻性研究[J]. 中国当代儿科杂志, 2021, 23(10): 1008-1014. PMID: 34719415. PMCID: PMC8549637. DOI: 10.7499/j.issn.1008-8830.2107103.
	Liu PY, Wang YB, Yang G, et al. The role of short-chain fatty acids in intestinal barrier function, inflammation, oxidative stress, and colonic carcinogenesis[J]. Pharmacol Res, 2021, 165: 105420. PMID: 33434620. DOI: 10.1016/j.phrs.2021.105420.
	Tang RQ, Li LJ. Modulation of short-chain fatty acids as potential therapy method for type 2 diabetes mellitus[J]. Can J Infect Dis Med Microbiol, 2021, 2021: 6632266. PMID: 33488888. PMCID: PMC7801078. DOI: 10.1155/2021/6632266.
	Liu JM, Jin YJ, Ye YL, et al. The neuroprotective effect of short chain fatty acids against sepsis-associated encephalopathy in mice[J]. Front Immunol, 2021, 12: 626894. PMID: 33584734. PMCID: PMC7876449. DOI: 10.3389/fimmu.2021.626894.
	Tong LC, Wang Y, Wang ZB, et al. Propionate ameliorates dextran sodium sulfate-induced colitis by improving intestinal barrier function and reducing inflammation and oxidative stress[J]. Front Pharmacol, 2016, 7: 253. PMID: 27574508. PMCID: PMC4983549. DOI: 10.3389/fphar.2016.00253.
	Li JJ, Zhang L, Wu T, et al. Indole-3-propionic acid improved the intestinal barrier by enhancing epithelial barrier and mucus barrier[J]. J Agric Food Chem, 2021, 69(5): 1487-1495. PMID: 33356219. DOI: 10.1021/acs.jafc.0c05205.
	Pace F, Rudolph SE, Chen Y, et al. The short-chain fatty acids propionate and butyrate augment adherent-invasive Escherichia coli virulence but repress inflammation in a human intestinal enteroid model of infection[J]. Microbiol Spectr, 2021, 9(2): e0136921. PMID: 34612688. PMCID: PMC8510176. DOI: 10.1128/Spectrum.01369-21.
	Kim CH. Control of lymphocyte functions by gut microbiota-derived short-chain fatty acids[J]. Cell Mol Immunol, 2021, 18(5): 1161-1171. PMID: 33850311. PMCID: PMC8093302. DOI: 10.1038/s41423-020-00625-0.
	Dupraz L, Magniez A, Rolhion N, et al. Gut microbiota-derived short-chain fatty acids regulate IL-17 production by mouse and human intestinal γδ T cells[J]. Cell Rep, 2021, 36(1): 109332. PMID: 34233192. DOI: 10.1016/j.celrep.2021.109332.
	Chen D, Gao ZQ, Wang YY, et al. Sodium propionate enhances Nrf2-mediated protective defense against oxidative stress and inflammation in lipopolysaccharide-induced neonatal mice[J]. J Inflamm Res, 2021, 14: 803-816. PMID: 33732006. PMCID: PMC7957230. DOI: 10.2147/JIR.S303105.