Objective To study the features of intestinal flora in children with food protein-induced proctocolitis (FPIP) by high-throughput sequencing. Methods A total of 31 children, aged <6 months, who experienced FPIP after exclusive breastfeeding and attended the outpatient service of the Third Affiliated Hospital of Zunyi Medical University from October 2018 to February 2021 were enrolled as the FPIP group. Thirty-one healthy infants were enrolled as the control group. Fecal samples were collected to extract DNA for PCR amplification. High-throughput sequencing was used to perform a bioinformatics analysis of 16S rDNA V3-V4 fragments in fecal samples. Results The diversity analysis of intestinal flora showed that compared with the control group, the FPIP group had a lower Shannon index for diversity (P>0.05) and a significantly higher Chao index for abundance (P<0.01). At the phylum level, the intestinal flora in both groups were composed of Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes. Compared with the control group, the FPIP group had a significant reduction in the composition ratio of Actinobacteria (P<0.001) and a significant increase in the composition ratio of Proteobacteria (P<0.05). At the genus level, the intestinal flora in the FPIP group were mainly composed of Escherichia, Clostridium, Enterococcus, Klebsiella, and Bifidobacterium, and the intestinal flora in the control group were mainly composed of Bifidobacterium and Streptococcus. Compared with the control group, the FPIP group had a significant reduction in the composition ratio of Bifidobacterium and Ruminococcus (P<0.05) and significant increases in the composition ratios of Clostridium and Shigella (P<0.05). Conclusions Compared with the control group, the FPIP group has a reduction in the diversity of intestinal flora and an increase in their abundance, and there are certain differences in several bacterial genera. These results suggest that changes in the composition of intestinal flora at genus level may play an important role in the development and progression of FPIP. Citation:Chinese Journal of Contemporary Pediatrics, 2022, 24(5): 536-542
Key words
Food protein-induced proctocolitis /
High-throughput sequencing /
Intestinal flora /
Child
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References
1 李小芹, 王菊平. 食物蛋白诱导的儿童过敏性直肠结肠炎研究进展[J]. 中国实用儿科杂志, 2021, 36(4): 257-261. DOI: 10.19538/j.ek2021040605.
2 Nowak-W?grzyn A. Food protein-induced enterocolitis syndrome and allergic proctocolitis[J]. Allergy Asthma Proc, 2015, 36(3): 172-184. PMID: 25976434. PMCID: PMC4405595. DOI: 10.2500/aap.2015.36.3811.
3 中华医学会儿科学分会消化学组. 食物过敏相关消化道疾病诊断与管理专家共识[J]. 中华儿科杂志, 2017, 55(7): 487-492. PMID: 28728255. DOI: 10.3760/cma.j.issn.0578-1310.2017.07.003.
4 Nowak-W?grzyn A, Katz Y, Mehr SS, et al. Non-IgE-mediated gastrointestinal food allergy[J]. J Allergy Clin Immunol, 2015, 135(5): 1114-1124. PMID: 25956013. DOI: 10.1016/j.jaci.2015.03.025.
5 Mennini M, Fiocchi AG, Cafarotti A, et al. Food protein-induced allergic proctocolitis in infants: literature review and proposal of a management protocol[J]. World Allergy Organ J, 2020, 13(10): 100471. PMID: 33072241. PMCID: PMC7549143. DOI: 10.1016/j.waojou.2020.100471.
6 Han P, Gu JQ, Li LS, et al. The association between intestinal bacteria and allergic diseases-cause or consequence?[J]. Front Cell Infect Microbiol, 2021, 11: 650893. PMID: 33937097. PMCID: PMC8083053. DOI: 10.3389/fcimb.2021.650893.
7 Penders J, Thijs C, van den Brandt PA, et al. Gut microbiota composition and development of atopic manifestations in infancy: the KOALA Birth Cohort Study[J]. Gut, 2007, 56(5): 661-667. PMID: 17047098. PMCID: PMC1942165. DOI: 10.1136/gut.2006.100164.
8 Marrs T, Flohr C. How do microbiota influence the development and natural history of eczema and food allergy?[J]. Pediatr Infect Dis J, 2016, 35(11): 1258-1261. PMID: 27518828. DOI: 10.1097/INF.0000000000001314.
9 Zimmermann P, Messina N, Mohn WW, et al. Association between the intestinal microbiota and allergic sensitization, eczema, and asthma: a systematic review[J]. J Allergy Clin Immunol, 2019, 143(2): 467-485. PMID: 30600099. DOI: 10.1016/j.jaci.2018.09.025.
10 Simonyté Sj?din K, Hammarstr?m ML, Rydén P, et al. Temporal and long-term gut microbiota variation in allergic disease: a prospective study from infancy to school age[J]. Allergy, 2019, 74(1): 176-185. PMID: 29786876. DOI: 10.1111/all.13485.
11 Berni Canani R, De Filippis F, Nocerino R, et al. Gut microbiota composition and butyrate production in children affected by non-IgE-mediated cow's milk allergy[J]. Sci Rep, 2018, 8(1): 12500. PMID: 30131575. PMCID: PMC6104073. DOI: 10.1038/s41598-018-30428-3.
12 Zhang Y, Xia G, Nie X, et al. Differences in manifestations and gut microbiota composition between patients with different Henoch-Schonlein purpura phenotypes[J]. Front Cell Infect Microbiol, 2021, 11: 641997. PMID: 34277463. PMCID: PMC8281929. DOI: 10.3389/fcimb.2021.641997.
13 Rogers MB, Firek B, Shi M, et al. Disruption of the microbiota across multiple body sites in critically ill children[J]. Microbiome, 2016, 4(1): 66. PMID: 28034303. PMCID: PMC5200963. DOI: 10.1186/s40168-016-0211-0.
14 Milani C, Duranti S, Bottacini F, et al. The first microbial colonizers of the human gut: composition, activities, and health implications of the infant gut microbiota[J]. Microbiol Mol Biol Rev, 2017, 81(4): e00036-17. PMID: 29118049. PMCID: PMC5706746. DOI: 10.1128/MMBR.00036-17.
15 Kourosh A, Luna RA, Balderas M, et al. Fecal microbiome signatures are different in food-allergic children compared to siblings and healthy children[J]. Pediatr Allergy Immunol, 2018, 29(5): 545-554. PMID: 29624747. DOI: 10.1111/pai.12904.
16 Qin J, Li R, Raes J, et al. A human gut microbial gene catalogue established by metagenomic sequencing[J]. Nature, 2010, 464(7285): 59-65. PMID: 20203603. PMCID: PMC3779803. DOI: 10.1038/nature08821.
17 Schade J, Weidenmaier C. Cell wall glycopolymers of Firmicutes and their role as nonprotein adhesins[J]. FEBS Lett, 2016, 590(21): 3758-3771. PMID: 27396949. DOI: 10.1002/1873-3468.12288.
18 王和平, 王玉蕾, 郑跃杰, 等. 湿疹患儿肠道菌群高通量测序初步探索[J]. 中国微生态学杂志, 2016, 28(7): 751-755. DOI: 10.13381/j.cnki.cjm.201607002.
19 Mennini M, Fierro V, Di Nardo G, et al. Microbiota in non-IgE-mediated food allergy[J]. Curr Opin Allergy Clin Immunol, 2020, 20(3): 323-328. PMID: 32250972. DOI: 10.1097/ACI.0000000000000644.
20 欧阳凤秀, 王旭. 生命早期肠道菌群的影响因素与儿童肥胖[J]. 上海交通大学学报(医学版), 2016, 36(9): 1378-1382. DOI: 10.3969/j.issn.1674-8115.2016.09.023.
21 Shin NR, Whon TW, Bae JW. Proteobacteria: microbial signature of dysbiosis in gut microbiota[J]. Trends Biotechnol, 2015, 33(9): 496-503. PMID: 26210164. DOI: 10.1016/j.tibtech.2015.06.011.
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