基于质谱技术的早产儿视网膜病血代谢产物的初步研究

doi:10.7499/j.issn.1008-8830.2209142

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Abstract Objective To study new biomarkers for the early diagnosis of retinopathy of prematurity (ROP) by analyzing the differences in blood metabolites based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) and metabolomics. Methods Dried blood spots were collected from 21 infants with ROP (ROP group) and 21 infants without ROP (non-ROP group) who were hospitalized in the Sixth Affiliated Hospital of Sun Yat-sen University from January 2013 to December 2016. LC-MS/MS was used to measure the metabolites, and orthogonal partial least squares-discriminant analysis was used to search for differentially expressed metabolites and biomarkers. Results There was a significant difference in blood metabolic profiles between the ROP and non-ROP groups. The pattern recognition analysis, Score-plot, and weight analysis obtained 10 amino acids with a relatively large difference. Further statistical analysis showed that the ROP group had significant increases in blood levels of glutamic acid, leucine, aspartic acid, ornithine, and glycine compared with the non-ROP group (P<0.05). The receiver operating characteristic curve analysis showed that glutamic acid and ornithine had the highest value in diagnosing ROP. Conclusions Blood metabolites in preterm infants with ROP are different from those without ROP. Glutamic acid and ornithine are the metabolic markers for diagnosing ROP. LC-MS/MS combined with metabolomics analysis has a potential application value in the early identification and diagnosis of ROP.

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	YANG Qiu-Ping
	LI Si-Tao
	HAO Hu
	GU Xia
	SHI Cong-Cong
	XIAO Xin
	CAI Yao

Key words： Retinopathy of prematurity Metabolomics Tandem mass spectrometry Liquid chromatography Biomarker Preterm infant

Received: 28 September 2022

Cite this article:

YANG Qiu-Ping,LI Si-Tao,HAO Hu et al. Blood metabolites in preterm infants with retinopathy of prematurity based on tandem mass spectrometry: a preliminary study[J]. CJCP, 2023, 25(2): 140-146.

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http://www.zgddek.com/EN/10.7499/j.issn.1008-8830.2209142 OR http://www.zgddek.com/EN/Y2023/V25/I2/140

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