基于HMGB1/NF-κB/NLRP3轴探讨褪黑素对氧诱导视网膜病变的保护作用

doi:10.7499/j.issn.1008-8830.2301036

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Abstract Objective To study the protective effect of melatonin (Mel) against oxygen-induced retinopathy (OIR) in neonatal mice and the role of the HMGB1/NF-κB/NLRP3 axis. Methods Neonatal C57BL/6J mice, aged 7 days, were randomly divided into a control group, a model group (OIR group), and a Mel treatment group (OIR+Mel group), with 9 mice in each group. The hyperoxia induction method was used to establish a model of OIR. Hematoxylin and eosin staining and retinal flat-mount preparation were used to observe retinal structure and neovascularization. Immunofluorescent staining was used to measure the expression of proteins and inflammatory factors associated with the HMGB1/NF-κB/NLRP3 axis and lymphocyte antigen 6G. Colorimetry was used to measure the activity of myeloperoxidase. Results The OIR group had destruction of retinal structure with a large perfusion-free area and neovascularization, while the OIR+Mel group had improvement in destruction of retinal structure with reductions in neovascularization and perfusion-free area. Compared with the control group, the OIR group had significant increases in the expression of proteins and inflammatory factors associated with the HMGB1/NF-κB/NLRP3 axis, the expression of lymphocyte antigen 6G, and the activity of myeloperoxidase (P<0.05). Compared with the OIR group, the OIR+Mel group had significant reductions in the above indices (P<0.05). Compared with the control group, the OIR group had significant reductions in the expression of melatonin receptors in the retina (P<0.05). Compared with the OIR group, the OIR+Mel group had significant increases in the expression of melatonin receptors (P<0.05). Conclusions Mel can alleviate OIR-induced retinal damage in neonatal mice by inhibiting the HMGB1/NF-κB/NLRP3 axis and may exert an effect through the melatonin receptor pathway.

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	Articles by authors
	CHU Fang-Fang
	ZHAO Yan-Song
	ZHAO Yu-Ze
	BAI Chen
	XIAO Pei-Lun
	WANG Xiao-Li
	YU Shu-Na
	JIANG Ji-Ying

Key words： Melatonin Oxygen-induced retinopathy HMGB1/NF-κB/NLRP3 axis Inflammation Neonatal mouse

Received: 12 January 2023

Corresponding Authors: Jiang J-Y，E-mail：jiangjy@wfmc.edu.cn E-mail: jiangjy@wfmc.edu.cn

Cite this article:

CHU Fang-Fang,ZHAO Yan-Song,ZHAO Yu-Ze et al. Protective effect of melatonin against oxygen-induced retinopathy: a study based on the HMGB1/NF-κB/NLRP3 axis[J]. CJCP, 2023, 25(6): 645-652.

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http://www.zgddek.com/EN/10.7499/j.issn.1008-8830.2301036 OR http://www.zgddek.com/EN/Y2023/V25/I6/645

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