Changes of CD4+CD25+ regulatory T cells, IL-10 and TGF-β1 levels in peripheral blood in children with asthma

LI Min, SONG Li, ZHANG Jian-Bo, FANG Jun, LI Lan

Chinese Journal of Contemporary Pediatrics ›› 2009, Vol. 11 ›› Issue (10) : 829-832.

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Chinese Journal of Contemporary Pediatrics ›› 2009, Vol. 11 ›› Issue (10) : 829-832.
CLINICAL RESEARCH

Changes of CD4+CD25+ regulatory T cells, IL-10 and TGF-β1 levels in peripheral blood in children with asthma

  • LI Min, SONG Li, ZHANG Jian-Bo, FANG Jun, LI Lan.
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Abstract

OBJECTIVE: This study determined the changes of CD4+CD25+ regulatory T cells, IL-10 and TGF-β1 levels in peripheral blood in children with asthma in order to explore immunologic mechanism of asthma attacks. METHODS: Seventy-four children with asthma attacks, twenty-eight children with stable asthma and twenty healthy children were enrolled. The percentage of CD4+CD25+ regulatory T cells in CD4+ T cells in peripheral blood was detected by flow cytometry. Serum levels of IL-10 and TGF-β1 were measured using enzyme linked immunosorbent assay (ELISA). RESULTS: The percentage of CD4+CD25+ regulatory T cells and IL-10 levels in peripheral blood in the asthma attacks group were significantly lower than those in the stable asthma and the control groups (P<0.01) .The stable asthma group showed a similar percentage of CD4+CD25+ regulatory T cells and IL-10 levels to the control group. Compared with the stable asthma and the control groups, serum TGF-β1 level in the asthma attacks group increased significantly (P<0.05). In the asthma attacks, the stable asthma, and the control groups, the percentage of CD4+CD25+ regulatory T cells in peripheral blood was not correlated to serum levels of IL-10 and TGF-β1. CONCLUSIONS: The percentage of CD4+CD25+ regulatory T cells in peripheral blood and serum IL-10 levels decrease and serum TGF-β1 levels increases in children with asthma attacks, which results in a decreased immunosuppressive effect. This might contribute to one of the immunologic mechanisms of asthma attacks.[Chin J Contemp Pediatr, 2009, 11 (10):829-832]

Key words

Asthma / Asthma attack / CD4+CD25+ regulatory T cell / IL-10 / TGF-β1 / Child

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LI Min, SONG Li, ZHANG Jian-Bo, FANG Jun, LI Lan. Changes of CD4+CD25+ regulatory T cells, IL-10 and TGF-β1 levels in peripheral blood in children with asthma[J]. Chinese Journal of Contemporary Pediatrics. 2009, 11(10): 829-832

References

[1]中华医学会儿科分会呼吸学组.儿童哮喘防治常规[J].中华儿科杂志,2004, 42(2):100-106.
[2]Rutkowski R, Moniuszko T, Stasiak-Barmuta A, Kosztyla Hojna B, Alifier M, Rutkowski K, et al. CD80 and CD86 expression on LPS-stimulated monocytes and the effect of CD80 and CD86 blockade on IL-4 and IFN-γ production in nonatopic bronchial asthma[J]. Arch Immunol Ther Exp, 2003, 51(6):421-428.
[3]Bryan SA, O′Connor BJ, Matti S, Lickie MJ, Kanabar V, Knan J, et al. Effects of recombinant human interleukin-12 on eosinophils, airway hyperresponsiveness, and the late asthmatic response[J]. Lancet, 2000, 356(9248):2149-2153.
[4]Sigurs N, Bjarnadon R, Sigurtergsion F, Kjellman B, Bjorksten B. Asthma and immunoglobulin E antibodies after respiratory syncytial virus bronchiolitis:a prospective cohort study with matched controls[J]. Pediatrics, 1995, 95(4):500-505.
[5]徐毛冶,黄茂.转化生长因子β1与支气管哮喘的关系[J].国外医学呼吸分册, 2005, 25(4):275-276.
[6]秦鑫,刘卓拉,李建强,薄建平,刘又宁.IFN-r和糖皮质激素对哮喘病人T淋巴细胞中的TGF-β的作用[J]. 实用医学杂志, 2006, 22(3):292-294.
[7]Moore KW, Malefyt R de Waal, Coffman RL, O′Garra A.Interleukin-10 and the interleukin-10 receptor[J]. Annu Rev Immunol, 2001, 19(1):683-765.
[8]Hawrylowicz CM, O′Garra A. Potential role of interleukin-10-secreting regulatory T cells in allergy and asthma[J]. Nat Rev Immunol, 2005, 5(4):271-283.
[9]Grissell TV, Powell H, Shafren DR, Boyle MJ, Hensley MJ, Jones PD, et al. Interleukin-10 gene expression in acute virus-induced asthma[J]. Am J Respir Crit Cure Med, 2005, 172(4):433-439.
[10]郑峻松,吴军,易绍萱,贺伟峰,陈希炜,解志杰,等. 细胞因子及胸腺微环境影响Treg细胞增殖机制研究[J]. 第三军医大学学报, 2003, 25(21):1885-1887.
[11]Jonuleit H, Schmitt E, Kakirman H, Stassen M, Knop J, Enk AH. Infectious tolerance: human CD25+ regulatory T cells convey suppressor activity to conventional CD4+ T helper cells[J]. J Exp Med, 2002, 196(2):255-260.
[12]Dieckmann D, Bruett CH, Ploettner H, Lutz MB, Schuler G. Human CD4+CD25+ regulatory, contact-dependent T cells induce interleukin 10-producing, contact-independent type 1-like regulatory T cells[J]. J Exp Med, 2002, 196(2):247-253.
[13]李敏,宋丽,张建波,房俊,李兰.糖皮质激素对哮喘小鼠CD4+CD25+调节性T细胞的作用[J].中国当代儿科杂志,2008,10(4):527-530.

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