
Expression of CD40 and CD40 ligand in peripheral blood mononuclear cells in children with bronchiolitis
ZHU Ping, ZOU Li-Ping
Chinese Journal of Contemporary Pediatrics ›› 2009, Vol. 11 ›› Issue (05) : 354-356.
Expression of CD40 and CD40 ligand in peripheral blood mononuclear cells in children with bronchiolitis
OBJECTIVE: The pathogenesis of bronchiolitis has not been fully identified. Immune function abnormality following virus infection may be associated with the pathogenesis. CD40 and CD40 ligand (CD40L) is a pair of co-stimulatory molecules in immunoreaction. They might play an important role in the development of bronchiolitis. This study aimed to investigate the expression of CD40 and CD40L in peripheral blood mononuclear cells (PBMCs) in children with bronchiolitis and explore their possible roles in the disease. METHODS: Thirty children with bronchiolitis, 26 children with bronchopneumonia and 30 healthy children (control) were enrolled. Flow cytometry was used to detect CD40 and CD40L expression in PBMCs. Total serum IgE level was measured using ELISA. RESULTS: Compared with the control group, CD40L expression significantly increased in the bronchiolitis and bronchopneumonia groups (P<0.05). The CD40L expression in the bronchiolitis group was significantly higher than that in the bronchopneumonia group (P<0.05). A significantly increased CD40 expression was also found in the bronchiolitis group when compared with the bronchopneumonia and the control group (P<0.01). Total serum IgE level in the bronchiolitis group was significantly higher than the bronchopneumonia and the control groups (P<0.01). CD40 and CD40L expression was positively correlated with serum IgE level in the bronchiolitis group (r=0.607, r=0.819, respectively; P<0.01). CONCLUSIONS: CD40 and CD40L expression in PBMCs and serum IgE level increased and there is a positive correlation between CD40 and CD40L expression and serum IgE level in children with bronchiolitis. Over-expression of CD40 and CD40L may play an important role in the development of bronchiolitis.[Chin J Contemp Pediatr, 2009, 11 (5):354-356]
Bronchiolitis / Peripheral blood mononuclear cell / CD40/CD40 ligand / Child
[1]胡亚美,江载芳.诸福棠实用儿科学[M].第7版.北京:人民卫生出版社,2002,1199-1200.
[2]Fleming DM, Pannell RS, Elliot AJ, Cross KW. Respiratory illness associated with influenza and respiratory syncytial virus infection[J]. Arch Dis Child, 2005, 90(7):741-746.
[3]Dakhama A, Park JW, Taube C, El Gazzar M, Kodama T, Miyahara N, et al. Alteration of airway neuropeptide expression and development of airway hyperresponsiveness following respiratory syncytial virus infection[J]. Am J Physiol Lung Cell Mol Physiol, 2005, 288(4): L761-770.
[4]黄海忠, 李颖红, 李玉华. 毛细支气管炎后早期干预对哮喘发生率的影响[J]. 实用医院临床杂志, 2006, 3(3): 41-42.
[5]Merendino AM, Bucchieri F, Gagliardo R, Daryadel A, Pompeo F, Chiappara G, et al. CD40 ligation protects bronchial epithelium against oxidantinduced caspase-independent cell death[J]. Am J Respir Cell Mol Biol, 2006, 35(2): 155-164.
[6]易学瑞, 孔祥平. CD40CD40L与疾病[J]. 国外医学·免疫学分册, 2001, 24(2): 80-82.
[7]Murugaiyan G, Martin S, Saha B. Levels of CD40 expression on dendritic cells dictate tumour growth or regression[J]. Clin Exp Immunol, 2007, 149(1):194-202.
[8]陈纯,吴燕峰,魏菁,方建培,黄绍良.CD40和CD40配体表达在异基因造血干细胞移植后移植物抗宿主病发生中的变化[J]. 实用儿科临床杂志, 2008, 23(3): 190-192.
[9]Caqnoni F, Oddera S, Giron-Michel J, Riccio AM, Olsson S, Dellacasa P, et al. CD40 on adult human airway epithelial cells: expression and proinflammatory effects[J]. J Immunol, 2004, 172(5):3205-3214.
[10]Kothlow S, Morgenroth I, Tregaskes CA, Kaspers B, Young JR. CD40 ligand supports the long-term maintenance and differentiation of chicken B cells in culture[J]. Dev Comp Immunol, 2008, 32(9):1015-1026.
[11]高武红,李成荣.婴儿急性毛细支气管炎随访分析[J].中国当代儿科杂志,2003,5(4):357-358.
[12]李兰,王智斌,李敏,张剑波,陈昌辉,李波,等.呼吸道合胞病毒毛细支气管炎患儿T细胞亚群检测的临床价值[J].中国当代儿科杂志,2005,7(5):421-422.
[13]刘筱萍, 胡晓艳, 曾宾, 钱卫疆, 王新根. 哮喘患儿淋巴细胞CD40/CD40 L信号分子的表达[J]. 中国当代儿科杂志, 2005, 7(3): 225-227.