Abstract OBJECTIVE: Some research has shown that p38 mitogen-activated protein kinase (p38MAPK) plays important roles in lung injuries induced by various factors. Its expression and role in hyperoxia-induced lung injury remains unknown. This study investigated the expression and role of p38MAPK in hyperoxia-induced lung injury juvenile rat model. METHODS: Hyperoxia-induced lung injury rat model was prepared by 90% O2 exposure. The location and expression of p38MAPK in lung tissues were detected by immunohistochemistry and Western blot respectively. Apoptosis index of lung was evaluated by TUNEL technique. The effect of SB203580, a p38MAPK inhibitor, on the apoptosis index of lung was observed. RESULTS: The expression of phosphor-p38MAPK increased obviously after hyperoxia. Positive phosphor-p38MAPK cells were mainly distributed in the alveolar, airway epithelial cells, pulmonary vascular endothelium cells and infiltrative inflammatory cells. The apoptosis index of lung also significantly elevated. SB203580 inhibited the activation of p38MAPK, and reduced the apoptosis index of lung. CONCLUSIONS: The phosphor-p38MAPK increased and was expressed in many kinds of lung cells in lung injury rat model. It may play a role in the induction of apoptosis in hyperoxia-induced lung injury.［Chin J Contemp Pediatr, 2009, 11 (5):389-392］
LI Jing,XU Feng,HU Lan et al. Expression and role of p38 mitogen-activated protein kinase in hyperoxia-induced lung injury juvenile rat model[J]. 中国当代儿科杂志, 2009, 11(05): 389-392.
LI Jing,XU Feng,HU Lan et al. Expression and role of p38 mitogen-activated protein kinase in hyperoxia-induced lung injury juvenile rat model[J]. CJCP, 2009, 11(05): 389-392.
［4］Han J, Lee JD, Tobias PS, Ulevitch RJ. Endotoxin induces rapid protein tyrosine phosphorylation in 70Z/3 cells expressing CD14［J］. J Biol Chem, 1993, 268(33):25009-25014.
［5］Zarubin T, Han J. Activation and signaling of the p38 MAP kinase pathway［J］.Cell Res, 2005, 15(1):11-18.
［6］Romashko J 3rd, Horowitz S, Franek WR, Palaia T, Miller EJ, Lin A, et al. MAPK pathways mediate hyperoxia-induced oncotic cell death in lung epithelial cells［J］.Free Radic Biol Med, 2003, 35(8):978-993.
［7］Lag M, Refsnes M, Lilleaas EM. Role of mitogen activated protein kinases and protein kinase C in cadmium-induced apoptosis of primary epithelial lung cells［J］. Toxicology, 2005, 211(3):253-264.
［8］Kim HJ, Lee HS, Chong YH, Kang JL. p38 mitogen-activated protein kinase up-regulates LPS-induced NF-kappaB activation in the development of lung injury and RAW 264.7 macrophages［J］. Toxicology, 2006, 225(1):36-47.
［9］May M, Strobel P, Preisshofen T, Seidenspinner S, Marx A, Speer CP. Apoptosis and proliferation in lungs of ventilated and oxygen-treated preterm infants［J］．Eur Respir J, 2004, 23（1）：113-121．
［11］McGrath-Morrow SA, Stahl J. Apoptosis in neonatal murine lung exposed to hyperoxia［J］. Am J Respir Cell Mol Biol, 2001, 25(2):150-155.
［12］Husari AW, Dbaibo GS, Bitar H , Khayat A, Panjarian S, Nasser M, et al. Apoptosis and the activity of ceramide,Bax and Bcl-2 in the lungs of neonatal rats exposed to limited and prolonged hyperoxia［J］. Respir Res, 2006, 7(1):100.
［13］Uhal BD. Apoptosis in lung fibrosis and repair［J］. Chest, 2002, 122(6): 293-298.
［14］Young LR, Pasula R, Gulleman PM, Deutsch GH, Mccormack FX. Susceptibility of Hermansky-Pudlak mice to bleomycin-induced type II cell apoptosis and fibrosis［J］. Am J Respir Cell Mol Biol, 2007, 37(1):67-74.
［15］Machino T, Hashimoto S, Maruoka S, Gon Y, Hayashi S, Mizumura K, et al.Apoptosis signal-regulating kinase 1-mediated signaling pathway regulates hydrogen peroxide-induced apoptosis in human pulmonary vascular endothelial cells［J］. Crit Care Med, 2003, 31(12):2776-2781.
［16］Carvalho H, Evelson P, Sigaud S, Gonzalez-Flecha B. Mitogen-activated protein kinases modulate H2O2-induced apoptosis in primary rat alveolar epithelial cells［J］. J Cell Biochem, 2004, 92(3)：502-513.
