Abstract Near-tetraploidy is a rare cytogenetic abnormality in myelocytic malignancies in children, and its significance is unknown. To investigate the characteristics of near-tetraploidy in a child with acute myelogenous leukemia (AML-M4), bone marrow smears were prepared for morphological analysis. Bone marrow samples were collected for flow cytometry, and prepared by short-term (24 hrs) unstimulated culture and R-banding for conventional cytogenetic assay. In this case, the morphological analysis of bone marrow cells showed large and prominent nuclei. The chromosomal analysis (R-banding) demonstrated a near-tetraploidy. Combined with morphological and immunophenotypic results, AML-M4 was confirmed. The patient was given four cycles of chemotherapy, and finally achieved clinical remission. However, the duration achieving the remission in the child was longer than AML children with normal karyotype. It is believed that near-tetraploid karyotype may have a great significance to the therapy and prognosis.［Chin J Contemp Pediatr, 2009, 11 (4):263-266］
ZHANG Ji-Hong,ZHENG Ying-Chun,WANG Yun-Xiu et al. Laboratory study on near-tetraploid acute myelogenous leukemia of childhood[J]. 中国当代儿科杂志, 2009, 11(04): 263-266.
ZHANG Ji-Hong,ZHENG Ying-Chun,WANG Yun-Xiu et al. Laboratory study on near-tetraploid acute myelogenous leukemia of childhood[J]. CJCP, 2009, 11(04): 263-266.
［1］Iyer RV, Sait SN, Matsui S, Block AW, Barcos M, Slack JL, et al. Massive hyperdiploidy and tetraploidy in acute myelocytic leukemia and myelodysplastic syndrome［J］. Cancer Genet Cytogenet, 2004, 148(1):29-34.
［2］Knuutila S, Simell O, Lipponen P, Saarinen I. Bone marrow chromosomes in healthy subjects［J］. Hereditas, 1976, 82(1):29-35.
［3］Walters R, Kantarjian HM, Keating MJ, Estey EH, Trujillo J, Cork A, et al. The importance of cytogenetic studies in adult acute lymphocytic leukemia［J］. Am J Med, 1990, 89(5):579-587.
［4］Yeh SP, Wang Y, Su J, Hsueh E, Yu M, Wu H. Near-tetraploid minimally differentiated acute myeloid leukemia with extensive erythrophagocytosis by leukemic blasts［J］. Ann Hematol, 2000, 79(1):36-39.
［5］Lemez P, Michalová K, Zemanová Z, Marinov I, Trpáková A, Moravcová J, et al. Three cases of near-tetraploid acute myeloid leukemias originating in pluripotent myeloid progenitors［J］. Leuk Res, 1998, 22(7):581-588.
［6］Au WY, Ma SK, Chan AC, Liang R, Lam CC, Kwong YL. Near tetraploidy in three cases of acute myeloid leukemia associated with mediastinal granulocytic sarcoma［J］. Cancer Genet Cytogenet, 1998, 102(1):50-53.
［7］Clarke MR, Lynch EF, Contis LC, Sherer ME, Shekhter Levin S. Near-tetraploidy in adult acute myelogenous leukemia［J］. Cancer Genet Cytogenet, 1996, 86(2):107-115.
［8］Kwong YL, Wong KF. Hyperdiploid acute myeloid leukemia: relationship between blast size and karyotype demonstrated by fluorescence in situ hybridization［J］. Cancer Genet Cytogenet, 1995, 83(1):1-4.
［9］Raimondi SC, Zhou Y, Shurtleff SA, Rubnitz JE, Pui CH, Behm FG. Near-triploidy and near-tetraploidy in childhood acute lymphoblastic leukemia: association with B-lineage blast cells carrying the ETV6-RUNX1 fusion,T-lineage immunophenotype, and favorable outcome［J］. Cancer Genet Cytogenet, 2006, 169(1):50-57.
［10］Xiao Z, Liu S, Liu X, Yu M, Hao Y.Tetraploidy or near-tetraploidy clones with double 8;21 translocation:a non-random additional anomaly of acute myeloid leukemia with t(8;21)(q22;q22) ［J］.Haematologica, 2005, 90(3):413-414.
［11］Dincol G, Palandüz S, Nalcaci M, Ucur A, Büyükaydin B.Myeloid/natural killer cell precursor acute leukemia with tetraploidy［J］.Cancer Genet Cytogenet, 2005, 163(2):156-159.
