Abstract OBJECTIVE: To study the effect of H2O2 on the proliferation and apoptosis of endothelial progenitor cells (EPCs) and the antogonistic effects of catechin on the cell apoptosis induced by H2O2 in rats. METHODS: Immuno-fluoreascence assay was applied to detect CD34, CD133 and VEGFR-2 expression. EPCs of generation 2 were divided into control cells, H2O2-treated cells and catechin-H2O2-treated cells (H2O2: 100 mg/L; catechin: 10 mg/L). Genomic DNA was extracted by the conventional method after intervention for the analysis of apoptosis ladder pattern. The MTT assay was applied to detect proliferation rate of EPCs. RESULTS: The cultured cells at day 10 expressed CD34, CD133 and VEGFR-2. DNA apoptosis ladder pattern appeared in H2O2 -treated cells 2 days after intervention. After 3 days of intervention DNA apoptosis ladder pattern appeared in both H2O2-treated cells and H2O2-catechin-treated cells, with more ladders and grayer scale in H2O2 -treated cells. Compared with the controls, H2O2-treated cells and H2O2-catechin-treated cells showed significantly decreased proliferation rate (P<0.01), with the lowest proliferation rate at the 2nd day (P<0.05). The H2O2-catechin-treated cells showed increased proliferation rate than H2O2-treated cells at the 1st, 2nd and 3rd days. CONCLUSIONS: H2O2 may impair EPCs proliferation and induce EPCs apoptosis. Catechin may increase the capacity of EPCs for the resistance to apoptosis induced by H2O2.［Chin J Contemp Pediatr, 2009, 11 (1):61-64］
CAO Yan,XU Zi-Chuan,HE Xiao-Jie et al. Protective effects of catechin on apoptosis of endothelial progenitor cells induced by H2O2 in rats[J]. 中国当代儿科杂志, 2009, 11(01): 61-64.
CAO Yan,XU Zi-Chuan,HE Xiao-Jie et al. Protective effects of catechin on apoptosis of endothelial progenitor cells induced by H2O2 in rats[J]. CJCP, 2009, 11(01): 61-64.
［1］Kang DH, Kanellis J, Hugo C, Truong L, Anderson S, Kerjaschki D, et al. Role of the microvascular endothelium in progressive renal disease［J］. J Am Soc Nephrol, 2002, 13(3):806-816.
［2］Ohashi R, Shimizu A, Masuda Y, Kitamura H, Ishizaki M, Sugisaki Y, et al. Capillary regression during the progression of experimental obstructive nephropathy［J］. J Am Soc Nephrol, 2002, 13(7): 1795-1805.
［3］Rizzoni D, Panza JA. Impaired endothelial regulation of vascular tone in patients with systemic arterial hypertension［J］. J Hypertens, 2006, 24(5):867-863.
［4］Mancini GB, Henry GC, Macaya C, O′Neill BJ, Pucillo AL, Carere RG, et al. Angiotensin-converting enzyme inhibition with quinapril improves endothelial vasomotor dysfunction in patients with coronary artery disease. The TREND (Trialon Reversing ENdothelial Dysfunction) study［J］. Circulation, 1996, 94(3):258-265.
［5］Ferroni P, Basili S, Paoletti V, Davì G. Endothelial dysfunction and oxidative stress in arterial hypertension［J］. Nutr Metab Cardiovasc Dis, 2006, 16(3):222-233.
［6］Fleck C, Schweitzer F, Karge E, Busch M, Stein G. Serum concentrations of asymmetric (ADMA) and symmetric (SDMA) dimethylarginine in patients with chronic kidney diseases［J］. Clin Chim Acta, 2003, 336(1-2):1-12.
［7］Jankowski J, van der Giet M, Jankowski V, Schmidt S, Hemeier M, Mahn B, et al. Increased plasma phenylacetic acid in patients with end-stage renal failure inhibits iNOS expression［J］. J Clin Invest, 2003, 112(2):256-264.
［8］Choi JH, Kim KL, Huh W, Kim B, Byun J, Suh W, et al. Decreased number and impaired angiogenic function of endothelial progenitor cells in patients with chronic renal failure［J］. Arterioscler Thromb Vasc Biol, 2004, 24(7):1246-1252.
［9］Asahara T, Murohara T, Sullivan A, Silver M, van der Zee R, Li T, et al. Isolation of putative progenitor endothelial cells for angiogenesis［J］. Science, 1977, 275(5302)：964-967.
［10］Jankun J, Selman SH, Swiercz R, Skrzypczak-Jankun E. Why drinking green tea could prevent cancer［J］. Nature, 1997, 387(6633):561-562.
［12］Kim MJ, Choi JH, Yang JA, Kim SY, Kim JH, Lee JH, et al. Effects of green tea catechin on enzyme activities and gene expression of antioxidative system in rat liver exposed to microwaves［J］. Nut Res, 2002, 22(6):733-744.
