非甲基化CpG-ODN对卵清蛋白致食物过敏幼鼠血清TGF-&beta;的影响及免疫调节作用

王本贞; 郑成中

doi:10.7499/j.issn.1008-8830.2015.08.020

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中国当代儿科杂志 ›› 2015, Vol. 17 ›› Issue (8) : 864-868. DOI: 10.7499/j.issn.1008-8830.2015.08.020

论著·实验研究

非甲基化CpG-ODN对卵清蛋白致食物过敏幼鼠血清TGF-β的影响及免疫调节作用

王本贞¹, 郑成中^1,2

作者信息 +

Effect of non-methylated CpG-ODN on serum TGF-β and immune regulation in ovalbumin-sensitized young mice

WANG Ben-Zhen¹, ZHENG Cheng-Zhong^1,2

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文章历史 +

摘要

目的探讨非甲基化胞嘧啶鸟嘌呤寡核苷酸(CpG-ODN)对卵清蛋白(OVA)致食物过敏幼鼠血清转化生长因子β(TGF-β)的影响及免疫调节作用。方法将30只2~3周龄BALB/c雌性小鼠随机分为对照组、模型组及非甲基化CpG-ODN干预组(CpG-ODN组),每组10只。采用OVA致敏建立幼鼠食物过敏动物模型,对照组小鼠以等量生理盐水替代,CpG-ODN组小鼠在每次OVA激发致敏前1 h腹腔注射非甲基化CpG-ODN溶液。观察各组过敏症状并评分;苏木精-伊红(HE)染色对空肠组织行病理检查;ELISA法检测小鼠血清OVA-IgE含量,CBA法检测小鼠血清中IL-4、IFN-γ、TGF-β含量。结果模型组小鼠出现过敏症状,空肠组织表现为I型变态反应病理特点;模型组和CpG-ODN组小鼠过敏症状评分高于对照组(P< 0.01);模型组血清中OVA-IgE、IL-4及TGF-β均高于对照组和CpG-ODN组(P< 0.05),且CpG-ODN组上述指标仍高于对照组(P< 0.05);模型组血清中IFN-γ水平低于对照组和CpG-ODN组(P< 0.05)。结论 OVA致食物过敏幼鼠血清TGF-β升高,TGF-β可能参与了过敏机制;非甲基化CpG-ODN可降低食物过敏幼鼠血清TGF-β水平,其在食物过敏中可能起到免疫调节作用。

Abstract

Objective To explore the effect of non-methylated cytosine-phosphate-guanine oligodeoxynucleotides (CpG-ODN) on serum transforming growth factor (TGF)-β and immune regulation in ovalbumin (OVA)-sensitized young mice. Methods Thirty female BALB/c mice (2-3 weeks old) were randomly divided into control, model, and CpG-ODN intervention groups. A young mouse model of food allergy was established by OVA sensitization. Normal saline of the same volume was used for replacement in the control group. The mice in the intervention group were intraperitoneally injected with CpG-ODN solution 1 hour before every OVA sensitization. Allergic symptoms were observed and scored for each group. The jejunal tissue was histopathologically examined with hematoxylin-eosin staining. Serum OVA-IgE level was measured using ELISA. Serum concentrations of interleukin (IL)-4, interferon (IFN)-γ, and TGF-β were determined by CBA. Results Allergic symptoms were observed in the model group and the jejunal tissue showed the pathological characteristics of type I allergic reaction. The allergic symptom scores in the model and CpG-ODN intervention groups were significantly higher than in the control group (P< 0.01). The serum levels of OVA-IgE, IL-4, and TGF-β were significantly higher in the model group than in the control and CpG-ODN intervention groups (P< 0.05). The CpG-ODN intervention group had significantly higher serum levels of OVA-IgE, IL-4, and TGF-β than the control group (P< 0.05). Compared with the control and CpG-ODN intervention groups, the model group had a significantly reduced IFN-γ level (P< 0.05). Conclusions The serum TGF-β level is increased in the young mouse model of OVA-sensitized food allergy and is involved in the allergy mechanism. Non-methylated CpG-ODN can reduce the serum TGF-β level in sensitized young mice and play an immunoregulatory role in food allergy.

导出引用

王本贞, 郑成中. 非甲基化CpG-ODN对卵清蛋白致食物过敏幼鼠血清TGF-β的影响及免疫调节作用[J]. 中国当代儿科杂志. 2015, 17(8): 864-868 https://doi.org/10.7499/j.issn.1008-8830.2015.08.020

WANG Ben-Zhen, ZHENG Cheng-Zhong. Effect of non-methylated CpG-ODN on serum TGF-β and immune regulation in ovalbumin-sensitized young mice[J]. Chinese Journal of Contemporary Pediatrics. 2015, 17(8): 864-868 https://doi.org/10.7499/j.issn.1008-8830.2015.08.020

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