肺炎患儿支气管肺泡灌洗液中肺表面活性蛋白A和D表达及其与肺炎临床特征的相关性

王鹂鹂; 郑首燕; 任洛; 肖秋艳; 龙晓茹; 罗健; 李渠北; 邓昱; 谢晓虹; 刘恩梅

doi:10.7499/j.issn.1008-8830.2016.05.002

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中国当代儿科杂志 ›› 2016, Vol. 18 ›› Issue (5) : 386-390. DOI: 10.7499/j.issn.1008-8830.2016.05.002

论著·临床研究

肺炎患儿支气管肺泡灌洗液中肺表面活性蛋白A和D表达及其与肺炎临床特征的相关性

王鹂鹂¹, 郑首燕¹, 任洛¹, 肖秋艳¹, 龙晓茹¹, 罗健², 李渠北², 邓昱², 谢晓虹², 刘恩梅²

作者信息 +

Levels of surfactant proteins A and D in bronchoalveolar lavage fluid of children with pneumonia and their relationships with clinical characteristics

WANG Li-Li¹, ZHENG Shou-Yan¹, REN Luo¹, XIAO Qiu-Yan¹, LONG Xiao-Ru¹, LUO Jian², LI Qu-Bei², DENG Yu², XIE Xiao-Hong², LIU En-Mei²

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文章历史 +

摘要

目的观察肺表面活性蛋白A、D(SP-A、SP-D)在肺炎患儿支气管肺泡灌洗液(BALF)中的表达,探讨SP-A、SP-D与肺炎患儿临床特征的相关性。方法 35例肺炎患儿纳入研究,对其肺泡灌洗液细胞进行分类计数,采用酶联免疫吸附试验(ELISA)检测患儿BALF中SP-A和SP-D水平。结果患儿BALF中SP-D水平显著高于SP-A水平(P<0.001)。BALF中SP-D水平与中性粒细胞百分比成负相关(r_s=-0.5255,P<0.01);血C反应蛋白水平升高(>8mg/L)的患儿BALF中SP-D水平明显低于C反应蛋白正常的患儿(P<0.05);有喘息症状的患儿BALF中SP-D水平明显低于无喘息症状者(P<0.01)。BALF中的SP-A与临床特征无明显关系(P>0.05)。结论肺炎患儿BALF中SP-D的表达显著高于SP-A,与肺炎患儿的临床特征有一定相关性,作为一种保护性因子在调节免疫及炎症反应中发挥着比SP-A更重要的作用。

Abstract

Objective To observe the levels of pulmonary surfactant proteins A and D (SP-A, SP-D) in bronchoalveolar lavage fluid (BALF) of children with pneumonia, and to explore their relationships with clinical characteristics. Methods Thirty-five children with pneumonia were enrolled in this study. Differential cell counts were obtained by Countstar counting board. The levels of SP-A and SP-D in BALF were detected using ELISA. Results In children with pneumonia, SP-D levels were significantly higher than SP-A levels (P<0.001). SP-D levels were negatively correlated with the neutrophil percentage in BALF (r_s=-0.5255, P<0.01). SP-D levels in BALF in children with increased blood C-reactive protein levels (>8 mg/L) were significantly lower than in those with a normal level of C-reactive protein (P<0.05). Compared with those in children without wheezing, SP-D levels in children with wheezing were significantly lower (P<0.01). There was no correlation between SP-A levels and clinical characteristics. Conclusions SP-D levels in BALF are significantly higher than SP-A levels, and have a certain correlation with clinical characteristics in children with pneumonia. As a protective factor, SP-D plays a more important role than SP-A in regulating the immune and inflammatory responses.

导出引用

王鹂鹂, 郑首燕, 任洛, 肖秋艳, 龙晓茹, 罗健, 李渠北, 邓昱, 谢晓虹, 刘恩梅. 肺炎患儿支气管肺泡灌洗液中肺表面活性蛋白A和D表达及其与肺炎临床特征的相关性[J]. 中国当代儿科杂志. 2016, 18(5): 386-390 https://doi.org/10.7499/j.issn.1008-8830.2016.05.002

WANG Li-Li, ZHENG Shou-Yan, REN Luo, XIAO Qiu-Yan, LONG Xiao-Ru, LUO Jian, LI Qu-Bei, DENG Yu, XIE Xiao-Hong, LIU En-Mei. Levels of surfactant proteins A and D in bronchoalveolar lavage fluid of children with pneumonia and their relationships with clinical characteristics[J]. Chinese Journal of Contemporary Pediatrics. 2016, 18(5): 386-390 https://doi.org/10.7499/j.issn.1008-8830.2016.05.002

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