哮喘患儿外周血中CD4+LAP+调节性T细胞比例及功能变化研究

罗景华; 杨良安; 李国林

doi:10.7499/j.issn.1008-8830.2016.08.011

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中国当代儿科杂志 ›› 2016, Vol. 18 ›› Issue (8) : 726-730. DOI: 10.7499/j.issn.1008-8830.2016.08.011

论著·临床研究

哮喘患儿外周血中CD4⁺LAP⁺调节性T细胞比例及功能变化研究

罗景华¹, 杨良安², 李国林¹

作者信息 +

Changes in proportion and function of peripheral CD4⁺LAP⁺ regulatory T cells in children with asthma

LUO Jing-Hua¹, YANG Liang-An², LI Guo-Lin¹

Author information +

文章历史 +

摘要

目的测定哮喘患儿外周血中CD4⁺LAP⁺调节性T细胞（CD4⁺LAP⁺Treg细胞）比例和功能的改变，探讨CD4⁺LAP⁺Treg细胞在哮喘发病中的作用。方法选取2014年3月至2015年9月确诊为哮喘的患儿75例为研究对象，依据病情将哮喘患儿分为哮喘急性期组（n=40）和哮喘缓解期组（n=35）；另选取行健康体检儿童30例为健康对照组。采用流式细胞仪检测各组儿童外周血CD4⁺LAP⁺Treg细胞百分比；采用3H-脱氧胸腺嘧啶苷法检测各组CD4⁺LAP⁺Treg细胞的免疫抑制情况。结果哮喘急性期组患儿体内CD4⁺LAP⁺Treg比例（2.0%±1.0%）较缓解期组（4.1%±2.4%）及健康对照组（4.6%±3.0%）均降低（P < 0.05）；哮喘急性期组患儿体内CD4⁺LAP⁺Treg细胞免疫抑制率（21%±4%）较缓解期组（55%±9%）及健康对照组（62%±11%）均降低（P < 0.05）。结论哮喘患儿外周血中CD4⁺LAP⁺Treg数量降低和抑制功能减弱可能参与了哮喘的急性发病过程。

Abstract

Objective To investigate the changes in the proportion and function of peripheral CD4⁺LAP⁺regulatory T cells (CD4⁺LAP⁺Treg cells) in children with asthma, as well as the role of CD4⁺LAP⁺Treg cells in the pathogenesis of asthma. Methods A total of 75 children who were diagnosed with asthma from March 2014 to September 2015 were enrolled as study subjects, and according to their conditions, they were divided into acute-stage asthma group (40 children) and remission-stage asthma group (35 patients). Another 30 children who underwent physical examination were enrolled as the healthy control group. Flow cytometry was used to determine the percentage of peripheral CD4⁺LAP⁺Treg cells, and[3H]-thymidine incorporation assay was performed to analyze the immunosuppression of CD4⁺LAP⁺Treg cells in each group. Results The acute-stage asthma group showed significant reductions in the proportion of CD4⁺LAP⁺Treg cells compared with the remission-stage asthma group and the healthy control group (2.0%±1.0% vs 4.1%±2.4%/4.6%±3.0%; P < 0.05). The acute-stage asthma group also showed a significant reduction in the immunosuppression rate of CD4⁺LAP⁺Treg cells compared with the remission-stage asthma group and the healthy control group (21%±4% vs 55%±9%/62%±11%; P < 0.05). Conclusions In children with asthma, the reduction in the number and inhibitory function of peripheral CD4⁺LAP⁺Treg cells may be involved in the pathogenesis of asthma.

导出引用

罗景华, 杨良安, 李国林. 哮喘患儿外周血中CD4⁺LAP⁺调节性T细胞比例及功能变化研究[J]. 中国当代儿科杂志. 2016, 18(8): 726-730 https://doi.org/10.7499/j.issn.1008-8830.2016.08.011

LUO Jing-Hua, YANG Liang-An, LI Guo-Lin. Changes in proportion and function of peripheral CD4⁺LAP⁺ regulatory T cells in children with asthma[J]. Chinese Journal of Contemporary Pediatrics. 2016, 18(8): 726-730 https://doi.org/10.7499/j.issn.1008-8830.2016.08.011

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