目的 探究微小RNA-142（microRNA-142, miR-142）在儿童自身免疫性甲状腺疾病（autoimmune thyroid disease, AITD）中表达及其与辅助性T细胞17（helper T cell 17, Th17）/调节性T细胞（regulatory T cell, Treg）失衡的关系。 方法 前瞻性选取2019年1月—2022年12月住院治疗的89例AITD患儿为研究对象，包括48例Graves病患儿（Graves disease, GD；设为GD组）和41例桥本甲状腺炎患儿（Hashimoto thyroiditis, HT；设为HT组），另选取55例同期体检的健康儿童为对照组，比较各组血清miR-142、甲状腺球蛋白抗体（antithyroglobulin antibody, TGAb）、抗甲状腺过氧化物酶抗体（antithyroperoxidase antibody, TPOAb）、Th17/Treg及白细胞介素-17（interleukin-17, IL-17）表达差异。 结果 GD组和HT组miR-142、TPOAb、TGAb、Th17、Th17/Treg、IL-17表达高于对照组，Treg水平低于对照组（P<0.05）。Pearson相关分析显示，GD组miR-142与TPOAb、TGAb、Th17、Th17/Treg、IL-17呈正相关（分别r=0.711、0.728、0.785、0.716、0.709，P<0.001），与Treg呈负相关（r=-0.725，P<0.001）；HT组miR-142与TPOAb、TGAb呈正相关（分别r=0.752、0.717，P<0.001）。 结论 miR-142在儿童AITD中高表达，且其表达可能与GD患儿Th17/Treg失衡有关。
Abstract
Objective To investigate the expression of microRNA-142 (miR-142) in children with autoimmune thyroid disease (AITD) and its relationship with the imbalance of helper T cell 17 (Th17) and regulatory T cell (Treg). Methods A total of 89 children hospitalized for AITD from January 2019 to December 2022 were prospectively selected as the study subjects, including 48 children with Graves' disease (GD group) and 41 children with Hashimoto's thyroiditis (HT group). Additionally, 55 healthy children undergoing physical examinations during the same period were selected as the control group. The differences in serum miR-142, antithyroglobulin antibody (TGAb), antithyroperoxidase antibody (TPOAb), Th17/Treg, and interleukin-17 (IL-17) expression were compared among the groups. Results The expression of miR-142, TPOAb, TGAb, Th17, Th17/Treg, and IL-17 in the GD group and HT group was higher than that in the control group, while Treg was lower than that in the control group (P<0.05). Pearson correlation analysis revealed that in the GD group, miR-142 was positively correlated with TPOAb, TGAb, Th17, Th17/Treg, and IL-17 (r=0.711, 0.728, 0.785, 0.716, 0.709, respectively; P<0.001) and negatively correlated with Treg (r=-0.725, P<0.001); in the HT group, miR-142 was positively correlated with TPOAb and TGAb (r=0.752, 0.717, respectively; P<0.001). Conclusions miR-142 is highly expressed in children with AITD, and its expression may be related to the Th17/Treg imbalance in children with GD.
关键词
自身免疫性甲状腺疾病 /
miR-142 /
免疫平衡 /
辅助性T细胞17 /
调节性T细胞 /
儿童
Key words
Autoimmune thyroid disease /
miR-142 /
Immune balance /
Helper T cell 17 /
Regulatory T cell /
Child
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基金
四川省科技计划资助（2022YFS0622）；泸州市人民政府-西南医科大学科技战略合作项目（2023LZXNYDJ047）。
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