高氧对新生大鼠海马区N-甲基-D-天冬氨酸受体1及其突触相关分子表达的影响

熊奕; 程琳; 蒋娜; 王团美; 薄涛

doi:10.7499/j.issn.1008-8830.2501086

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中国当代儿科杂志 ›› 2025, Vol. 27 ›› Issue (8) : 1002-1010. DOI: 10.7499/j.issn.1008-8830.2501086

论著·实验研究

高氧对新生大鼠海马区N-甲基-D-天冬氨酸受体1及其突触相关分子表达的影响

熊奕 ¹ ,
程琳 ¹ ,
蒋娜 ² ,
王团美 ² ,
薄涛 ¹

作者信息 +

Effects of hyperoxia on the expression of hippocampal N-methyl D-aspartate receptor 1 and its synapse-associated molecules in neonatal rats

Yi XIONG ¹ ,
Lin CHENG ¹ ,
Na JIANG ² ,
Tuan-Mei WANG ² ,
Tao BO ¹

Author information +

文章历史 +

摘要

目的探究高氧对新生大鼠海马区N-甲基-D-天冬氨酸受体1（N-methyl-D-aspartate receptor 1, NMDAR1）及其突触相关分子大麻素受体1（cannabinoid receptor 1, CB1R）、突触后致密物（postsynaptic density, PSD）95及突触素（synapsin, SYN）表达的影响。方法选取1日龄Sprague-Dawley新生大鼠，分为高氧组和对照组（n=8），高氧组持续吸入80%±5%氧气，对照组吸入空气，干预7 d。分别于高氧暴露后1、3、7 d，采用苏木精-伊红染色观察脑组织病理学改变，免疫组织化学染色、蛋白质印迹法及实时荧光定量PCR检测海马区NMDAR1、CB1R、PSD95和SYN蛋白及mRNA表达变化。结果高氧暴露7 d时，高氧组脑组织神经细胞密度降低，排列紊乱。与对照组相比，高氧暴露1 d时，高氧组CB1R mRNA及NMDAR1、CB1R蛋白表达显著下调，SYN蛋白表达显著上调（P<0.05）；高氧暴露3 d时，高氧组NMDAR1、CB1R及SYN mRNA表达显著下调（P<0.05），NMDAR1、CB1R蛋白表达显著下调（P<0.05），PSD95、SYN蛋白表达显著上调（P<0.05）；高氧暴露7 d时，高氧组NMDAR1、CB1R蛋白表达显著上调（P<0.05）。结论持续高氧暴露可导致新生大鼠海马区NMDAR1及其突触相关分子的表达水平发生时间依赖性改变。

Abstract

Objective To investigate the effects of hyperoxia on the expression of N-methyl-D-aspartate receptor 1 (NMDAR1) and its synapse-associated molecules, including cannabinoid receptor 1 (CB1R), postsynaptic density 95 (PSD95), and synapsin (SYN), in the hippocampus of neonatal rats. Methods One-day-old Sprague-Dawley neonatal rats were randomly divided into a hyperoxia group and a control group (n=8 per group). The hyperoxia group was exposed to 80% ± 5% oxygen continuously, while the control group was exposed to room air, for 7 days. At 1, 3, and 7 days after hyperoxia exposure, hematoxylin and eosin (HE) staining was used to observe histopathological changes in the brain. The expression levels of NMDAR1, CB1R, PSD95, and SYN proteins and mRNAs in the hippocampus were detected by immunohistochemistry, Western blotting, and quantitative real-time PCR. Results After 7 days of hyperoxia exposure, the hyperoxia group showed decreased neuronal density and disordered arrangement in brain tissue. Compared with the control group, after 1 day of hyperoxia exposure, CB1R mRNA and both NMDAR1 and CB1R protein expression in the hyperoxia group were significantly downregulated, while SYN protein expression was significantly upregulated (P<0.05). After 3 days, mRNA expression of NMDAR1, CB1R, and SYN was significantly decreased (P<0.05); NMDAR1 and CB1R protein expression was significantly downregulated (P<0.05), while PSD95 and SYN protein expression was significantly upregulated (P<0.05). After 7 days of hyperoxia, the protein expression of NMDAR1 and CB1R was significantly upregulated (P<0.05). Conclusions Continuous hyperoxia exposure induces time-dependent changes in the expression levels of NMDAR1 and its synapse-associated molecules in the hippocampus of neonatal rats.

导出引用

熊奕, 程琳, 蒋娜, 等. 高氧对新生大鼠海马区N-甲基-D-天冬氨酸受体1及其突触相关分子表达的影响[J]. 中国当代儿科杂志. 2025, 27(8): 1002-1010 https://doi.org/10.7499/j.issn.1008-8830.2501086

Yi XIONG, Lin CHENG, Na JIANG, et al. Effects of hyperoxia on the expression of hippocampal N-methyl D-aspartate receptor 1 and its synapse-associated molecules in neonatal rats[J]. Chinese Journal of Contemporary Pediatrics. 2025, 27(8): 1002-1010 https://doi.org/10.7499/j.issn.1008-8830.2501086

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