目的　了解肿瘤坏死因子 α (TNF α)在百日咳菌液导致的感染性脑水肿 (感脑 )时的变化及与感脑脑损害的关系。方法　 99只大鼠随机分成空白对照组 (C组 ,n =11) ;生理盐水组 (NS组 ,n =44 ) ;百日咳菌液脑水肿组 (PB组 ,n =44 )。NS组和PB组又分为 30 ,6 0 ,12 0 ,2 40min 4个亚组。用RT PCR和ELISA方法检测不同时间大鼠感脑脑组织匀浆中的TNF αmRNA表达及蛋白质生成水平。结果　TNF α含量在PB 2 40min组 (76 8.4± 86 .3pg/ g)显著增加 ,分别与C组 (318.1± 31.1pg/ g) ,NS 30min(32 4.2± 6 5 .6 pg/ g) ,6 0min(32 2 .1± 73.4pg/g) ,12 0min(316 .8± 49.1pg/g) ,2 40min(32 9.9± 77.6 pg/g)及PB 30min(36 0 .3± 6 6 .6 pg/g) ,6 0min(35 8.5±49.1pg/ g) ,12 0min(35 6 .0± 5 2 .3pg/ g)组比较差异均有非常显著性 (P <0 .0 1)。TNF αmRNA表达信号在PB6 0min组开始增加 ,并随注菌后时间的增加 ,而逐渐增强 ,2 40min达高峰。结论　感脑时脑组织中的TNF α含量显著增加 ,提示TNF α与感脑脑损害密切相关。

Objective To understand the relationship between tumor necrotic factor α (TNF α) and brain damage in an animal model of pertussis bacilli induced brain edema. Methods 99 rats were divided into 3 groups: 1)sham control group (C group, n=11); 2)normal saline group (NS group, n=44); and 3) pertussis bacilli group (PB group, n=44). TNF α mRNA expression was examined using RT PCR and the protein levels were assayed using ELISA in the rat brain tissues at various times. Results TNF α was significantly increased in the PB group at 240 min ( 768.4 ± 86.3 pg/g) compared to the C group ( 318.1± 31.1 pg/g), NS group at 30 min ( 324.2 ± 65.6 pg/g), NS group at 60 min ( 322.1± 73.4 pg/g), NS group at 120 min ( 316.8 ± 49.1 pg/g), and NS group at 240 min ( 329.9 ± 77.6 pg/g). The PB group at 240 minutes was also significantly increased compared to the PB group at 30 min ( 360.3 ± 66.6 pg/g), at 60 min ( 358.5 ± 49.1 pg/g), and at 120 min ( 356.0 ± 52.3 pg/g) (P< 0.01 ). Expression of TNF α mRNA began to increase in the PB group at 60 min and reached a peak at 240 min. Conclusions TNF α may play a role in producing injury in infectious brain edema.