目的：研究人巨细胞病毒(HCMV)，单纯疱疹病毒Ⅱ型(HSVⅡ)及人微小病毒B19(HPV B19)感染与胎儿畸形的关系。方法：应用聚合酶链反应技术检测了畸形组16例和对照组25例围生期死亡病例的脑、肝、肺、肾、脾组织和妊母胎盘组织中的HCMV，HSVⅡ及HPV B19 DNA。结果：①畸形组HCMV，HSVⅡ，HPV B19阳性率分别为：50.0%(8/16)，37.5%(6/16)和31.3%(5/16)，而对照组阳性率分别为8.0%(2/25)，8.0%(2/25)，12.0%(3/25)，两组之间CMV，HSVⅡ感染有显著性差异(P<0.05)。②HCMV易感器官为脑、肝、肺，畸形组中脑组织阳性率43.8%(7/16)与肾组织6.3%(1/16)有显著性差异，HSVⅡ易感器官主要为肝脏，HPV-B19主要为脾脏受累。结论：HCMV，HSVⅡ胎儿与畸形有密切关系，HPV-B19感染因例数较少与畸形无明显关系。

OBJECTIVE: To explore the relationship between cytomegalovirus (CMV), herpes simplex virus (HSV) and human parvovirus B19 (HPV-B19) and fetal malformation. METHODS: There were 16 infants with congenital malformation in the malformation group and 25 normal infants in the control group. CMV, HSV and HPV-B19 infections were diagnosed using the polymerase chain reaction technique (PCR) in the main organs and placentas of the 2 groups. RESULTS: The infection rate with CMV and HSV in the malformation group was 50.0% and 37.5% respectively, compared with the 8.0% and 8.0% respectively in the control group. (P<0.05). The HPV-B19 infection rate didn't differ in the 2 groups. The brain, liver and lungs were the organs most commonly involved in CMV infection, whereas in HSV infection the liver was most commonly involved, with HPV-B19 most commonly affecting the spleen. CONCLUSIONS: CMV and HSV infections are closely related to congenital malformation, but HPV B19 infection is not related to malformation.