Abstract：OBJECTIVE: To find the best pressure and time of hyperbaric oxygenation (HBO) therapy by comparing different pressures and times in a rat model of neonatal hypoxic ischemic brain damage (HIBD) and to provide the mechanisms of HBO therapy for neonatal clinical application. METHODS: 7day old SD rats were randomly divided into a control(CON) group, HIBD group and HBO group. The HBO group was divided into 7 subgroups according to the different HBO pressure: 1 ATA(H 1.0)，1.25 ATA(H 1.25), 1.5 ATA(H 1.5), 1.75 ATA(H 1.75), 2 ATA(H 2.0), 2.25 ATA(H 2.25) and 2.5 ATA(H 2.5) group. After choosing the best pressure we divided the rats into two groups according to the different HBO time: 30 min group (H 30 min) and 60 min group (H 60 min). All the rats(14dayold) were killed at 7 days after HI. Body weight increasing, left/right brain weight ratio, neuropathologic examination and superoxide dismutase(SOD) and malondialdehyde(MDA) content in brain tissue were measured in each group. RESULTS: ①The weight increasing rate ［(72.91±21.27)%］ and the left/right brain weight ratio (0.6444±0.1599) decreased obviously. At the same time the content of SOD ［(203.32±57.18) NU/ml］ in brain tissue decreased and that of MDA ［(4.197±0.683) nmol/ml］ increased in HIBD group. All above data showed a more significant difference in HIBD group compared to the control group ［weight increasing rate=(104.97±17.34)%，left/right brain weight ratio=0.9857±0.0396，SOD content=(261.53±41.38) NU/ml，MDA content=(3.393±0.574) nmol/ml］(P0.05). CONCLUSIONS: HBO of 2.0 ATA pressure and 30～60 min steady time may have the best effect, and reasonable HBO treatment can increase SOD content and decrease MDA content in brain tissue in HIBD neonatal rats.
LIU Li Xu,YANG Yu Jia. Relationship of Dose-Effect and Time-Effect to Hyperbaric Oxygen Therapy on Hypoxic-Ischemic Brain Damage in the Neonatal Rat[J]. 中国当代儿科杂志, 2001, 3(4): 450-454.
LIU Li Xu,YANG Yu Jia. Relationship of Dose-Effect and Time-Effect to Hyperbaric Oxygen Therapy on HypoxicIschemic Brain Damage in the Neonatal Rat[J]. CJCP, 2001, 3(4): 450-454.
