
染色体6q16.3区域一个与儿童急性淋巴细胞白血病相关表达序列标记的鉴定
康睿, 曹励之, 谢岷, 陈英
中国当代儿科杂志 ›› 2005, Vol. 7 ›› Issue (2) : 147-149.
染色体6q16.3区域一个与儿童急性淋巴细胞白血病相关表达序列标记的鉴定
Identification of the expressed sequence tags associated with childhood acute lymphoblastic leukemia at chromosome 6q16.3
目的:寻找染色体6q16. 3区域与儿童急性淋巴细胞性白血病(ALL)相关的表达序列标记(EST),为筛选儿童ALL候选基因打下基础。方法:运用生物信息学同源性比较筛选EST的策略,结合逆转录PCR方法,检测6q16. 3区域的相关EST在儿童ALL淋巴细胞和正常淋巴细胞中的表达。结果:在6q16. 3区域筛选到一个在儿童ALL细胞中低表达的ESTAA403058,与正常淋巴细胞相比较,在10例儿童ALL淋巴细胞中的7例其表达下调(P<0. 001)。结论:染色体6q16. 3区域ESTAA403058在儿童ALL中表达下调,提示其可能参与儿童ALL癌变过程,为克隆儿童ALL相关基因提供了新线索。
OBJECTIVE: To identify the expressed sequence tags (EST) associated with childhood acute lymphoblastic leukemia (ALL) at chromosome 6q16.3, so as to clone candidate genes at the locus. METHODS: The expression of EST at 6q16.3 in lymphocytes was detected using EST homology analysis in bioinformatics along with reverse transcription-polymerase chain reaction in 10 children with ALL and 5 healthy children. RESULTS: The EST AA403058 expression decreased in seven of ten ALL children compared with that in healthy children (P<0.001). CONCLUSIONS: The EST AA403058 expression is down-regulated in childhood ALL, suggesting it may be involved in the development of childhood ALL. This study provided important information toward cloning childhood ALL associated genes.
表达序列标记 / 白血病 / 淋巴细胞 / 急性 / 儿童 / 6q16.3 / 基因表达
Expressed sequence tags / Lymphoblastic leukemia, acute, childhood / 6q16.3 / Gene expression