目的：S100A4基因在神经母细胞瘤早期转移中发挥重要作用。该研究以脂质体介导S100A4基因反义寡核苷酸转染体外培养的神经母细胞瘤细胞,观察其作用效率及稳定性。方法：以脂质体包裹5’端带有FAM荧光标记的S100A4基因反义寡核苷酸转染体外培养的神经母细胞瘤细胞,用无脂质体包裹的反义寡核苷酸为对照,在激光共聚焦显微镜下观察荧光强度变化、作用效率及存留时间。结果：经脂质体介导的S100A4基因反义寡核苷酸可以高效进入神经母细胞瘤细胞,荧光强度转染后8 h达最高值173,24 h后仍高达135,作用效率达68%,稳定表达72 h以上;无脂质体包裹的反义寡核苷酸荧光强度在转染后6 h达最高值90,作用效率35%,稳定表达仅12 h。结论：脂质体介导的S100A4基因反义寡核苷酸作用于神经母细胞瘤细胞效率高、时间持久,值得在神经母细胞瘤基因治疗中进一步探讨。

OBJECTIVE: S100A4 gene plays an important role in neuroblastoma cell invasion and metastasis. This paper aimed to evaluate the liposome-mediated transfection efficacy of S100A4 antisense oligodeoxynucleotide (AS-ODN) and its stability in neuroblastoma cells. METHODS: The fluorescence (FAM) labeled S100A4 AS-ODN was transfected with Lipofectamine~(TM) 2000 into human neuroblastoma cells (LA-N-5). The transfection efficacy, stability and persistence time were observed by laser scanning confocal microscopy, and were compared with those of naked AS-ODN without Lipofectamine~(TM) 2000. RESULTS: The transfection efficacy of the S100A4 AS-ODN mediated by Lipofectamine~(TM) 2000 in neuroblastoma cells was high.The maximal signal intensity of intracellular fluorescence was 173 at 8 hrs after transfection and remained as high as 135 at 24 hrs. The maximal efficacy of transfection was 68%, and the stable expression lasted for more than 72 hrs. The transfection efficacy of the naked AS-ODN without Lipofectamine~(TM) 2000 was 35%. The maximal signal intensity of intracellular fluorescence was 90 and occurred at 6 hrs after transfection. The stable expression lasted for only 12 hrs. CONCLUSIONS: The S100A4 AS-ODN can be effectively transfected with Lipofectamine~(TM) 2000 into neuroblastoma cells, and be stably expressed,which may be useful in gene therapy for neuroblastoma