目的：探讨Kiss-1在雌性性早熟大鼠不同时期下丘脑中mRNA的表达。方法：正常5日龄清洁级Sprague-Dawley雌性大鼠40只，随机分为4组，对照1组 （正常青春前期），对照2组（正常青春期早期），模型1组（青春期早期），模型2组（青春期中期），每组10只，达那唑300 μg皮下注射以诱导真性性早熟。半定量RTPCR法检测对照1组，对照2组，模型1组，模型2组的Kiss-1 mRNA的表达。结果：与对照1组相比，模型1组和模型2组下丘脑Kiss-1 mRNA水平分别增加了1.4和2.8倍，差异具有显著性（P<0.05）。模型2组大鼠下丘脑Kiss-1 mRNA表达水平高于模型1组，差异有显著性（P<0.05）。 对照2组与模型1组的Kiss-1 mRNA的表达差异无显著性（P>0.05）。结论：Kiss-1 mRNA的表达与生长发育的时期密切相关，提示Kiss-1 可能与真性性早熟的发生有关。［中国当代儿科杂志，2007，9（1）：59-62］

OBJECTIVE: To investigate the gene expression of Kiss-1 in the hypothalamus of true precocious female rats at various stages of development. METHODS: Forty 5-day-old normal female Sprague-Dawley rats were randomly assigned into four groups of 10 rats: Control group 1, Control group 2, Model group 1 and Model group 2. The rats from the two model groups were injected with 300 μg of danazol at 5 days of age to induce true precocious puberty. The two control groups were injected with normal saline instead. For the determination of Kiss-1 mRNA expression in the hypothalamus, the rats of the Model group 1 were sacrificed during the first diestrus (early puberty) and meanwhile the rats of the Control group 1 were sacrificed when they were at prepuberty; the Control group 2 rats were sacrificed at the first diestrus (early puberty); the rats of the Model group 2 were sacrificed during the second diestrus (middle puberty). The expression of Kiss-1 mRNA in the hypothalamus in the four groups was detected using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). RESULTS: Kiss-1 mRNA expression in the hypothalamus in Model group 1 and Model group 2 increased by 1.4-fold and 2.8-fold, respectively, compared with that of Control group 1 (P<0.05). Model group 2 showed significantly higher Kiss-1 mRNA levels than Model group 1 (P<0.05). There were no statistical differences in the Kiss-1 mRNA expression between Control group 2 and Model group 1. CONCLUSIONS: Gene expression of Kiss-1 is associated with the developmental period of true precicous puberty, suggesting that Kiss-1 might play a role in the pathogenesis of this disorder.［Chin J Contemp Pediatr, 2006, 9(1):59-62］