目的：近年来研究表明细胞间黏附分子整合素的变化与肿瘤细胞的侵袭迁移之间有着重要的联系，该研究进一步深入探讨α2β1整合素对神经母细胞瘤细胞侵袭和迁移能力的影响，为预防神经母细胞瘤细胞的转移和辅助治疗提供理论依据。方法：采用细胞倾斜实验、聚碳酸酯膜侵袭试验观察神经母细胞瘤SK-N-SH细胞株细胞在整合素α2单克隆抗体(anti-α2)和β1单克隆抗体(anti-β1)作用下，瘤细胞迁移、侵袭能力的变化。迁移或侵袭的细胞通过姬姆萨染色,200倍显微镜下计数，计算不同条件下细胞迁移或侵袭抑制率。结果：anti-α2和anti-β1阻断组细胞的迁移能力较对照组98.1±7.4明显降低，分别为50.9±10.5和54.3±9.0(P<0.01)，抑制率分别为48.1%和44.5%。anti-α2和anti-β1阻断组细胞的侵袭能力较对照组41.5±4.8明显减弱，分别为25.3±4.4和18.8±3.9(P<0.01)，抑制率分别为39.0%和54.7%；结论：α2β1整合素在神经母细胞瘤细胞迁移侵袭过程中起着促进作用。

OBJECTIVE: To study the effect of integrin α2β1 on invasion and migration of SK-N-SH neuroblastoma cells.METHODS: Neuroblastoma SK-N-SH cell line was cultured in the modified eagle's medium. The effects of monoclonal antibodies to integrin α2 and integrin β1 on migration and invasion were measured by inclined test and polycarbonate filters incorporated in modified Transwell chambers respectively．The migration and invasion cells were stained with Gimsa staining and counted under a 200 multiplied microscope. The blocking rate of migration and invasion of cells was calculated.RESULTS: The number of migrated SK-N-SH cells in the anti-α2 and anti-β1 treatment groups (50.9±10.5 and 54.3±9.0 respectively) was significantly less than that in the control group without monoclonal antibody treatment (98.1±7.4) (P<0.01), with a blocking rate of cell migration of 48.1% and 44.5% respectively. The invasion to matrigel of SK-N-SH cells exposed monoclonal antibodies to integrin α2 and integrin β1 was significantly blocked compared with the control SK-N-SH cells, with the number of invasion cells in the anti-α2 and anti-β1 treatment groups of 25.3 ± 4.4 and 18.8 ± 3.9 respectively vs 41.5 ± 4.8 in the control group (P<0.01). The blocking rate of cell invasion in the anti-α2 and anti-β1 treatment groups was 39.0% and 54.7% respectively.CONCLUSIONS: Integrin α2β1 may promote migration and invasion of neuroblastoma cells.