PDF(948 KB)
PDF(948 KB)
PDF(948 KB)
癫癎患儿外周血单个核细胞NF-κb活化的变化
NF-kappa B activation in peripheral blood mononuclear cells of children with epilepsy
目的：探讨癫癎患儿外周血单个核细胞中核因子κB(nuclear factor-kappa B, NF-κB)活化的变化及其临床意义。方法：采用流式细胞学方法检测84例癫癎患儿治疗前后及32例正常儿的外周血单个核细胞中NF-κB的活化情况。结果：各实验癫癎组患儿外周血单个核细胞中NF-κB的活化均明显高于健康对照组（P<0.05，或<0.01），差异具有显著性意义；全身发作组和分类不明组的癫癎患儿外周血单个核细胞中NF-κB的活化明显高于部分发作组（P<0.01），且全身发作组高于分类不明组（P<0.05）。癫癎患儿部分发作组、全身发作组及分类不明组在治疗后外周血单个核细胞中NF-κB的活化明显降低（P<0.01），差异具有极其显著性意义。结论：癫癎发作患儿外周血单个核细胞NF-κB的激活是增加的，与发作的严重程度呈正相关。［中国当代儿科杂志，2009，11（1）：44-46］
OBJECTIVE: To study the changes and significance of NF-kappa B activation in peripheral blood mononuclear cells (PBMC) of children with epilepsy. METHODS: NF-kappa B activation in PBMC was assayed by the flow cytometry in 32 healthy children and 64 children with epilepsy before and after treatment. The 64 epileptic children were subdivided into three groups: systemic seizure, partial seizure and unknown classification. RESULTS: NF-kappa B activation in PBMC in three epilepsy subgroups were significantly higher than that in healthy controls. The systemic seizure group showed significantly increased NF-kappa B activation in PBMC compared with the partial seizure group (P<0.01) and the unknown classification group (P<0.05). After treatment NF-kappa B activation in PBMC in three epilepsy subgroups was significantly reduced (P<0.01). CONCLUSIONS: NF-kappa B activation in PBMC increased in children with epilepsy, and it was positively correlated with the severity of seizures.［Chin J Contemp Pediatr, 2009, 11 (1):44-46］
癫癎 / 核因子κB / 单个核细胞 / 流式细胞学 / 儿童
Epilepsy / NF-kappa B / Mononuclear cell / Flow cytometry / Child
［1］彭建霞，庞保东，徐应军，曹丽华，董琰，刘寅. 原发性癫癎患儿治疗前后血清细胞因子水平的变化及意义［J］.实用医学杂志，2007，23（8）：1157-1158.
［2］张茜, 白洁, 张琳娜, 彭彦洪. 氧化苦参碱对青霉素致痫大鼠血清中细胞因子的影响［J］.宁夏医学院学报，2006，28（3）：202-206.
［3］Mattson MP, Meffert MK. Roles for NK-kappaB in nerve cell survival, plasticity, and disease［J］.Cell Death Differ, 2006, 13(5):852-860.
［4］Heckscher ES, Fetter RD, Marek KW, Albin SD, Davis GW. NF-kappaB, IkappaB, and IRAK control glutamate receptor density at the Drosophila NMJ［J］. Neuron, 2007, 55(6):859-873.
［5］Aya K, AlhawagriM, Hagen-Stapleton A, Kitaura H, Kanaqawa O, Novack DV. NF-( kappa)B-inducing kinase controls lymphocyte and osteoclast activities in inflammatory arthritis［J］. J Clin Invest, 2005, 115(7):1848-1854.
［6］Blondeau N, Widmann C, Lazdunski M, Heurteaux C. Activation of the nuclear factor-kappa B is a key event in brain tolerance［J］.J Neurosci, 2001, 21(13): 4668-4677.
［7］Voutsinos-Porche B, Koning E, Kaplan H, Ferrandon A, Guenounou M, Nehlig A, et al, Temporal patterns of the cerebral inflammatory response in the rat lithium-pilocarpine model of temporal lobe epilepsy［J］. Neurobiol Dis, 2004, 17(3):385-402.
［8］Crespel A, Coubes P, Rouaset MC, Brana C, Rougier A, Rondouin G, et al．Inflammatory reactions in human medial temporal lobe epilepsy with hippocampal selerosis［J］. Brain Res, 2002, 952(2)：159-169．
［9］黄亚玲, 孙丹, 刘亚黎．戊四氮反复点燃致不同发育期大鼠海马损伤的病理改变［J］．中华儿科杂志, 2005, 43(12)：930-934．
［10］李卫华, 唐荣华, 张宇红. 戊四氮及NF-κB圈套结构对神经元样细胞突触素表达的影响［J］.华中科技大学学报（医学版），2006，35（4）：429-432.
［11］余春涛，刘智胜，董宗祈，肖诗豹.热性惊厥患儿血清细胞因子水平的变化及临床意义［J］.中国当代儿科杂志，1999，1（2）：84-86.
［12］刘智胜，王芳琳，杨立志，王慧燕.年龄依赖性癫癎性脑病患儿的血清细胞因子变化［J］.中国当代儿科杂志，2000，2（5）：308-310.
