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哮喘患儿外周血T淋巴细胞上前列腺素D2受体改变研究
Changes of prostaglandin D2 receptor on T cells in peripheral blood of children with asthma
目的：哮喘的慢性气道炎症与TH2的极化有关，炎症介质前列腺素D2（PGD2）可通过T淋巴细胞上前列腺素D2受体（DP1/CRTH2）调控其极化和分泌功能，而且CRTH2是TH2细胞的特征性受体，研究不同哮喘病期T淋巴细胞上受体改变，对了解哮喘患儿中TH2极化状态和哮喘发病机制有重要意义。方法：正常对照组35例，哮喘急性发作期组42例和临床缓解期组30例。用酶联免疫吸附法测定外周血IL-4，IL-5 和IFN-γ，用放射配体结合分析法测定外周血T细胞上DP1/CRTH2结合容量。结果：哮喘急性发作期和临床缓解期组T淋巴细胞上总结合和CRTH2受体结合容量比正常对照组显著增加（P<0.01）；DP1差异无显著性。哮喘急性发作期和临床缓解期组TH2细胞因子IL-4、IL-5比正常对照组显著增加（P<0.01）；TH1细胞因子IFN-γ比正常对照组显著减少（P<0.01）。结论：支气管哮喘病人临床缓解期和急性发作期外周血T淋巴细胞上CRTH2受体表达增加，外周血中TH2细胞因子分泌增加， TH1细胞因子分泌减少, 提示在哮喘病人中存在TH2的极化状态，应用CRTH2拮抗剂可能是以后哮喘治疗的一个新靶点。［中国当代儿科杂志，2009，11（3）：199-202］
OBJECTIVE: Chronic airway inflammation is associated with the polarization of TH2 cells in asthma. Prostaglandin D2 (PGD2) plays an important role in the polarization of TH2 cells. This study aimed to investigate the changes of PGD2 receptors (DP1/CRTH2) on T lymphocytes and their significance in asthma. METHODS: Seventy-two children with asthma were assigned to two groups: acute attack (n=42) and remission (n=30). Thirty-five healthy children were used as the control group. Plasma levels of TH2 cytokines IL-4 and IL-5, and TH1 cytokine INF-γ were detected using ELISA. Radiological binding assay (RBA) was used to measure the contents of DP1/CRTH2 receptors on T cells in peripheral blood (PPB). RESULTS: The total combining contents of DP and CRTH2 on T cells in PPB in the acute attack and the remission groups were significantly higher than those in the control group (P<0.01). There was no significant difference in the DP1 content among the three groups. Serum levels of IL-4 and IL-5 significantly increased (P<0.01), in contrast, serum levels of TH1 cytokine IFN-γ were significantly reduced in the acute attack and the remission groups compared with those in the control group (P<0.01). CONCLUSIONS:The total combining contents of DP and CRTH2 on T cells increased, serum levels of TH2 cytokines also increased, but serum levels of TH1 cytokine decreased significantly in the acute attack and the remission phases in children with asthma. This showed that a polarization of TH2 cells occurred in children with asthma and suggested that CRTH2 antagonism may be a new target for the treatment of asthma.［Chin J Contemp Pediatr, 2009, 11 (3):199-202］
Asthma / Lymphocyte / CRTH2 / Child
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