NYGGF4基因过表达对脂肪细胞胰岛素敏感性及分泌功能的影响

张春梅; 邱洁; 陈小慧; 王玢; 张敏; 郭锡熔

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中国当代儿科杂志 ›› 2009, Vol. 11 ›› Issue (10) : 846-849.

论著·实验研究

NYGGF4基因过表达对脂肪细胞胰岛素敏感性及分泌功能的影响

张春梅，邱洁，陈小慧，王玢，张敏，郭锡熔

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Effects of NYGGF4 gene over-expression on the insulin sensitivity and secretory function of adipocytes

ZHANG Chun-Mei, QIU Jie, CHEN Xiao-Hui, WANG Bin, ZHANG Min, GUO Xi-Rong.

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摘要

目的：观察肥胖相关新基因NYGGF4过表达对脂肪细胞的胰岛素敏感性及分泌功能的影响。方法：体外培养稳定转染NYGGF4基因（NYGGF4-pcDNA3.1）的3T3-L1前体脂肪细胞，以转染空载体（pcDNA 3.1）的3T3-L1细胞为对照，胰岛素加地塞米松加1-甲基-3-异丁基黄嘌呤(MDI)方案诱导细胞分化成熟，液闪仪测定3H标记的葡萄糖摄取率，Western blot检测葡萄糖转运体4（GLUT4）的表达及转位；采用ELISA双抗体夹心法检测培养上清中TNF-α、IL-6、脂联素、抵抗素4种细胞因子的水平。结果：NYGGF4过表达显著降低胰岛素刺激的葡萄糖摄取率（P<0.05）。NYGGF4过表达对总的GLUT4的表达量没有影响，但明显降低胰岛素刺激的GLUT4由细胞浆向细胞膜的转位（P<0.05）。过表达NYGGF4的脂肪细胞其培养上清中TNF-α、IL-6、脂联素及抵抗素的分泌水平与对照组相比差异无显著性（P>0.05）。结论：NYGGF4基因过表达通过下调胰岛素刺激的GLUT4的转位而减少脂肪细胞的葡萄糖摄取率，提示脂肪细胞对胰岛素的敏感性降低，而对脂肪细胞的分泌功能无明显影响。［中国当代儿科杂志，2009，11（10）：846-849］

Abstract

OBJECTIVE: To study the effect of a new obesity-related gene NYGGF4 on the insulin sensitivity and secretory function of adipocytes. METHODS: 3T3-L1 preadipocytes transfected with either an empty expression vector (pcDNA3.1; control group) or an NYGGF4 expression vector (NYGGF4-pcDNA3.1) were cultured in vitro and differentiated into the matured adipocytes with the standard insulin plus dexamethasone plus 3-isobutyl-methylxanthine (MDI) induction cocktail. 2-deoxy-D-［3H］ glucose uptake was determined by liquid scintillation counting. Western blot was performed to detect the protein content and translocation of glucose transporter 4 (GLUT4). The supernatant concentrations of TNF-α, IL-6, adiponectin and resistin were measured using ELISA. RESULTS: NYGGF4 over-expression in 3T3-L1 adipocytes reduced insulin-stimulated glucose uptake. NYGGF4 over-expression impaired insulin-stimulated GLUT4 translocation without affecting the total protein content of GLUT4. The concentrations of TNF-α, IL-6, adiponectin and resistin in the culture medium of 3T3-L1 transfected with NYGGF4 were not significantly different from those in the control group. CONCLUSIONS: NYGGF4 over-expression impairs the insulin sensitivity of 3T3-L1 adipocytes through decreasing GLUT4 translocation and had no effects on the secretory function of adipocytes.［Chin J Contemp Pediatr, 2009, 11 (10):846-849］

导出引用

张春梅, 邱洁, 陈小慧, 王玢, 张敏, 郭锡熔. NYGGF4基因过表达对脂肪细胞胰岛素敏感性及分泌功能的影响[J]. 中国当代儿科杂志. 2009, 11(10): 846-849

ZHANG Chun-Mei, QIU Jie, CHEN Xiao-Hui, WANG Bin, ZHANG Min, GUO Ti-Rong. Effects of NYGGF4 gene over-expression on the insulin sensitivity and secretory function of adipocytes[J]. Chinese Journal of Contemporary Pediatrics. 2009, 11(10): 846-849

中图分类号： R335

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