TNFα对人脂肪细胞STEAP4基因表达的调控

陈小慧; 赵亚萍; 朱春; 季晨博; 张春梅; 朱金改; 高春林; 郭锡熔

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中国当代儿科杂志 ›› 2009, Vol. 11 ›› Issue (12) : 1008-1011.

论著·实验研究

TNFα对人脂肪细胞STEAP4基因表达的调控

陈小慧，赵亚萍，朱春，季晨博，张春梅，朱金改，高春林，郭锡熔

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Regulative role of TNFα on STEAP4 gene in matured human adipocytes

CHEN Xiao-Hui, ZHAO Ya-Ping, ZHU Chun, JI Chen-Bo, ZHANG Chun-Mei, ZHU Jin-Gai, GAO Chun-Lin, GUO Xi-Rong

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摘要

目的：人STEAP4基因是一个参与胰岛素敏感性调节的肥胖相关差异表达基因，本研究旨在探讨胰岛素抵抗相关脂源性细胞因子TNFα对人成熟脂肪细胞中STEAP4基因的调节作用。方法：体外培养人前体脂肪细胞，在诱导人前体脂肪细胞分化成熟的基础上，应用不同浓度（0、5、10、25、50 ng/mL）人重组TNFα干预成熟脂肪细胞24 h，采用实时荧光定量RT-PCR技术、Western blot技术检测干预后人成熟脂肪细胞STEAP4基因在核酸和蛋白表达水平的变化。结果：不同浓度TNFα（5、10、25、50 ng/mL）干预24 h能显著上调人成熟脂肪细胞中STEAP4基因的mRNA表达,与未干预对照组差异有显著性（P<0.05）；50 ng/mL TNFα干预时STEAP4基因在人成熟脂肪细胞中的mRNA表达水平最高。与基因表达结果相一致，不同浓度TNFα（5、10、25、50 ng/mL）干预24 h能显著上调人成熟脂肪细胞中STEAP4蛋白的表达,与未干预对照组差异有显著性（P<0.05）；干预浓度提高到25 ng/mL 时干预效果最为明显。结论：TNFα能显著上调人成熟脂肪细胞中STEAP4基因核酸和蛋白的表达。［中国当代儿科杂志，2009，11（12）：1008-1011］

Abstract

OBJECTIVE: Human STEAP4, a novel obesity-related gene, is associated with insulin sensitivity regulation in human adipocytes. This study aimed to explore the regulative role of TNFα on STEAP4 gene in matured human adipocytes. METHODS: Human preadipocytes were cultured and differentiated into matured adipocytes in vitro. Fully differentiated adipocytes (Day 17) were treated with different concentrations of TNFα (0, 5, 10, 25 and 50 ng/mL) for 24 hrs. Total RNA and protein were extracted from the adipocytes. Levels of STEAP4 mRNA and protein expression were determined by real-time quantitative RT-PCR and Western blot respectively. RESULTS: Different concentrations (5, 10, 25 and 50 ng/mL) of TNFα treatment for 24 hrs resulted in a significant increase in the STEAP4 mRNA expression of human matured adipocytes.The maximal effect was seen in the 50 ng/mL of TNFα treatment group. In parallel, STEAP4 protein synthesis in matured adipocytes increased in response to TNFα treatment of different concentrations (5, 10, 25 and 50 ng/mL) for 24 hrs. The maximal up-regulated effect was seen in the 25 ng/mL of TNFα treatment group. CONCLUSIONS: TNFα can up-regulate STEAP4 mRNA expression in human matured adipocytes.［Chin J Contemp Pediatr, 2009, 11 (12):1008-1011］

导出引用

陈小慧, 赵亚萍, 朱春, 季晨博, 张春梅, 朱金改, 高春林, 郭锡熔. TNFα对人脂肪细胞STEAP4基因表达的调控[J]. 中国当代儿科杂志. 2009, 11(12): 1008-1011

CHEN Xiao-Hui, ZHAO Ya-Ping, ZHU Chun, JI Chen-Bo, ZHANG Chun-Mei, ZHU Jin-Gai,. Regulative role of TNFα on STEAP4 gene in matured human adipocytes[J]. Chinese Journal of Contemporary Pediatrics. 2009, 11(12): 1008-1011

中图分类号： R-33

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