整合素连接激酶表达与儿童紫癜性肾炎肾小球损害的关系

李志辉; 张翼; 银燕; 何金华; 段翠蓉; 寻劢; 刘志群

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中国当代儿科杂志 ›› 2009, Vol. 11 ›› Issue (12) : 888-891.

论著·临床研究

整合素连接激酶表达与儿童紫癜性肾炎肾小球损害的关系

李志辉，张翼，银燕，何金华，段翠蓉，寻劢，刘志群

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Relationship between integrin-linked kinase expression and renal glomerular damage in children with Henoch-Sch-nlein purpura nephritis

LI Zhi-Hui, ZHANG Yi, YIN Yan, HE Jin-Hua, DUAN Cui-Rong, XIN Mai, LIU Zhi-Qun

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摘要

目的:研究表明整合素连接激酶（integrinlinked kinase, ILK）在肾脏疾病的发生、发展中起着重要作用。该研究旨在探讨ILK与儿童紫癜性肾炎（HSPN）肾小球损害的关系。方法:188例HSPN患儿按照国际儿童肾脏病组织（ISKDC）关于HSPN肾脏病理分级分成5组，即≤Ⅱa级组、Ⅱb级组、Ⅲa级组、Ⅲb级组、≥Ⅳ级组。对照组为薄基膜肾病患儿。采用免疫组织化学方法检测对照组及HSPN患儿肾小球ILK的表达，分析肾小球ILK表达与肾小球病理损害、尿蛋白排泄量之间的关系。结果:①ILK在肾小球的表达：对照、≤Ⅱa级、Ⅱb级、Ⅲa级、Ⅲb级、≥Ⅳ级组6组患儿肾小球ILK的阳性表达面积分别为：（3.35±1.01）%、（4.88±1.13）%、（9.64±1.36）%、（11.27±1.68）%、（17.42±3.0）%、（20.62±2.32）%，6组间比较，差异有非常显著性（P<0.01）。②随着患儿尿蛋白排泄量的增加，肾小球ILK表达逐渐增强，尿蛋白阴性组、尿微量白蛋白组、显著尿蛋白组及大量尿蛋白组间的ILK表达差异有非常显著性（P<0.01）。结论: ILK与儿童HSPN肾小球病理损害及蛋白尿的发生密切相关。［中国当代儿科杂志，2009，11（11）：888-891］

Abstract

OBJECTIVE: Recent studies have shown that integrin-linked kinase (ILK) plays an important role in the pathogenesis and development of some kidney diseases. This study aimed to investigate the relationship between ILK and renal glomerular damage in children with Henoch-schonlein purpura nephritis (HSPN). METHODS: One hundred and eighty-eight HSPN children (aged 3 to 17 years) were assigned to five groups according to the classification of the International Study of Kidney Disease in Children (ISKDC): grade ≤IIa (n=62), grade IIb(n=42), grade IIIa(n=29), grade IIIb(n=40) and grade≥IV(n=15). Fifteen children with basement membrane nephropathy served as the control group. ILK expression on glomeruli was ascertained by immunohistochemical staining. The relationships of ILK expression on glomeruli with glomerular histopathologic lesions and urinary protein excretions were examined. RESULTS: The positive areas of ILK expression on glomeruli in the control, grade≤IIa, grade IIb, grade IIIa, grade IIIb and grade≥IV groups were (3.35±1.01)%, (4.88±1.13)%, (9.64±1.36)%, (11.27±1.68)%, (17.42±3.0)% and (20.62±2.32%), respectively. There were significant differences in the ILK expression between groups (P<0.01). ILK expression on glomeruli increased with increased urinary protein excretions. There were significant differences in the ILK expression in children with different urinary protein excretions (P<0.01). CONCLUSIONS: ILK might be involved in the process of renal glomerular histopathologic damage and the production of proteinuria in children with HSPN.［Chin J Contemp Pediatr, 2009, 11 (11):888-891］

导出引用

李志辉, 张翼, 银燕, 何金华, 段翠蓉, 寻劢, 刘志群. 整合素连接激酶表达与儿童紫癜性肾炎肾小球损害的关系[J]. 中国当代儿科杂志. 2009, 11(12): 888-891

LI Zhi-Hui, ZHANG Yi, YIN Yan, HE Jin-Hua, DUAN Cui-Rong, XIN Mai, LIU Zhi-Qun. Relationship between integrin-linked kinase expression and renal glomerular damage in children with Henoch-Sch-nlein purpura nephritis[J]. Chinese Journal of Contemporary Pediatrics. 2009, 11(12): 888-891

中图分类号： R692.3+4

参考文献

［1］Legate KR, Montanez E, Kudlacek O, Fassler R. ILK, PINCH and parvin: the tIPP of integrin signalling［J］. Nat Rev Mol Cell Biol, 2006, 7（1）:20-31.
［2］Sepulveda JL, Wu C. The parvins［J］. Cell Mol Life Sci, 2006, 63（1）:25-35.
［3］Vespa A, D′Souza SJ, Dagnino L. A novel role for integrin-linked kinase in epithelial sheet morphogenesis［J］. Mol Biol Cell, 2005, 16（9）:4084-4095.
［4］Qian Y, Zhong X, Flynn DC. ILK mediates actin filament rearrangements and cell migration and invasion through PI3K/Akt/Rac1 signaling［J］. Oncogene, 2005, 24（19）:3154-3165.
［5］Vouret-Craviari V, Boulter E, Grall D, Matthews C, Van Obberghen-Schilling E. ILK is required for the assembly of matrix-forming adhesions and capillary morphogenesis in endothelial cells［J］. J Cell Sci, 2004, 117（Pt 19）:4559-4569.
［6］Friedrich EB, Liu E, Sinha S, Cook S, Milstone DS, MacRae CA, et al. Integrin-linked kinase regulates endothelial cell survival and vascular development［J］. Mol Cell Biol, 2004, 24（18）:8134-8144.
［7］Vouret-Craviari V, Boulter E, Grall D, Matthews C, Van Obberghen-Schilling E. ILK is required for the assembly of matrix-forming adhesions and capillary morphogenesis in endothelial cells［J］. J Cell Sci, 2004, 117（Pt 19）:4559-4569.
［8］Wu C. The PINCH-ILK-parvin complexes: assembly, functions and regulation［J］. Biochim Biophys Acta, 2004, 1692(2-3):55-62.
［9］Yamaji S, Suzuki A, Kanamori H, Mishima W, Yoshimi R, Takasaki H, et al. Affixin interacts with alpha-actinin and mediates integrin signaling for reorganization of F-actin induced by initial cell-substrate interaction［J］. J Cell Biol, 2004, 165(4):539-551.
［10］Lee YI, Kwon YJ, Joo CK. Integrin-linked kinase function is required for transforming growth factor beta-mediated epithelial to mesenchymal transition［J］. Biochem Biophys Res Commun, 2004, 316(4):997-1001.
［11］李志辉. 整合素连接激酶介导大鼠肾小球系膜细胞生物学行为的变化［J］. 实用儿科临床杂志，2007, 22（5）：330-332.
［12］李志辉，吴天慧，段翠蓉，何金华，银燕. 血管紧张素Ⅱ上调大鼠肾小球系膜细胞整合素连接激酶的表达［J］. 实用儿科临床杂志，2008, 23（5）：343-345.
［13］中华医学会儿科学分会肾脏病学组. 小儿肾小球疾病的临床分类、诊断及治疗［J］. 中华儿科杂志，2001，39（12）：746-749.
［14］Guo L, Sanders PW, Woods A, Wu C. The distribution and regulation of integrin-linked kinase in normal and diabetic kidneys［J］. Am J Pathol, 2001, 159(5):1735-1742.
［15］Li ZH, Chen XM, Xie YS, Shi S, Feng Z, Fu B, et al. Expression and significance of integrin-linked kinase in cultured cells, normal tissue, and diseased tissue of aging rat kidneys［J］. J Gerontol A Biol Sci Med Sci, 2004, 59(10):984-996.
［16］景红. 整合素连接激酶在肾脏疾病中的作用［J］. 中国当代儿科杂志，2007, 9（6）: 619-621.
［17］Kretzler M, Teixeira VP, Unschuld PG, Cohen CD, Wanke R, Edenhofer I, et al. Integrin-linked kinase as a candidate downstream effector in proteinuria［J］. FASEB J, 2001,15(10):1843-1845.