91例EB病毒相关疾病儿童血浆EB病毒DNA的检测

段红梅; 姚瑶; 谢正德; 闫静; 胡英慧; 幺远; 周玲; 申昆玲

PDF(1001 KB)

中国当代儿科杂志 ›› 2009, Vol. 11 ›› Issue (12) : 897-900.

论著·临床研究

91例EB病毒相关疾病儿童血浆EB病毒DNA的检测

段红梅，姚瑶，谢正德，闫静，胡英慧，幺远，周玲，申昆玲

作者信息 +

Determination of plasma EBV DNA in 91 children with EBV-associated diseases

DUAN Hong-Mei, YAO Yao, XIE Zheng-De, YAN Jing, HU Ying-Hui, YAO Yuan, ZHOU Ling, SHEN Kun-Ling

Author information +

文章历史 +

摘要

目的:了解EB病毒（EBV）感染患儿外周血血浆中游离EBV DNA的拷贝数，确定EBV原发感染后外周血血浆中EBV游离DNA的拷贝数与发病天数及病情轻重的关系。方法:应用荧光定量PCR方法，测定73例EBV原发感染和18例EBV相关重症疾病患儿外周血血浆中EBV游离DNA。结果:①原发EBV感染患儿外周血血浆中EBV游离DNA随发病天数呈下降趋势，发病2周后很难检测到。②EBV相关重症疾病组患儿外周血血浆中EBV游离DNA阳性率明显高于原发EBV感染组，差异有显著性(89% vs 16%, P＜0.05）。结论: 原发EBV感染后随病程天数的增加，病毒复制水平逐渐下降。血浆中EBV游离DNA检测对评价EBV相关疾病的严重程度有一定参考价值。［中国当代儿科杂志，2009，11（11）：897-900］

Abstract

Objective: To determine the plasma level of Epstein-Barr virus (EBV) DNA in children with EBV-associated diseases, and to investigate the dynamic changes of EBV DNA level after initial infection as well as the relationship between EBV-DNA level and the diseases severity. Methods: The subjects consisted of 73 children with primary EBV infection (infectious mononucleosis, pneumonia，etc.) and 18 children with severe EBV-associated diseases (chronic active EBV infection, hemophagocytic lymphohistiocytosis, etc.). The plasma EBV DNA level was detected by a real-time PCR assay．Results: The plasma EBV DNA level decreased with the infection time in children with primary EBV infection. Two weeks after infection, plasma EBV DNA was almost undetectable. The positive rate of plasma EBV DNA in children with severe EBV-associated diseases increased significantly when compared with that in children with primary EBV infection (89% vs 16%; P<0.05). Conclusions: The level of EBV replication may be reduced with the infection time. Dynamic determination of blood EBV DNA is useful for the evaluation of disease severity in children with EBVinfection.［Chin J Contemp Pediatr, 2009, 11 (11):897-900］

导出引用

段红梅, 姚瑶, 谢正德, 闫静, 胡英慧, 幺远, 周玲, 申昆玲. 91例EB病毒相关疾病儿童血浆EB病毒DNA的检测[J]. 中国当代儿科杂志. 2009, 11(12): 897-900

DUAN Hong-Mei, YAO Yao, XIE Zheng-De, YAN Jing, HU Ying-Hui, YAO Yuan, ZHOU Ling, SHEN Kun-Ling. Determination of plasma EBV DNA in 91 children with EBV-associated diseases[J]. Chinese Journal of Contemporary Pediatrics. 2009, 11(12): 897-900

中图分类号： R373 R446.1

参考文献

［1］Fafi-Kremer S, Morand P, Brion JP, Pavese P, Baccard M, Germi R, et al. Long-term shedding of infectious epstein-barr virus after infectious mononucleosis［J］.J Infect Dis, 2005, 191(6):985-989.
［2］Cohen JI. Epstein-Barr virus infection［J］. N Engl J Med, 2000, 343(7):481-492.
［3］段红梅，谢正德，申昆玲. 慢性活动性EB病毒感染的研究进展［J］. 临床儿科杂志，2007，25（2）：153-156.
［4］Kimura H, Hoshino Y, Kanegane H, Tsuge I, Okamura T, Kawa K, et al. Clinical and virologic characteristics of chronic active Epstein-Barr virus infection［J］. Blood, 2001, 98(2):280-286.
［5］谢正德，段红梅，张辉，闫静，刘亚谊，申昆玲. 儿童EB病毒相关性噬血淋巴组织细胞增生症的病毒学特征［J］. 首都医科大学学报，2005，26（5）：568-570.
［6］段红梅，谢正德，申昆玲. EB病毒相关组织淋巴细胞增生性噬血综合征研究进展［J］. 临床儿科杂志，2005，23(7):425-430.
［7］段红梅，申昆玲. 慢性活动性EB病毒感染的诊断标准和治疗［J］.实用儿科临床杂志，2006，21（10）：638-640.
［8］陆华，诸美瞻，胡亚美. 传染性单核细胞增多症［M］．／／胡亚美，江载芳．诸福棠实用儿科学. 上册．第7版．北京：人民卫生出版社，2002， 821-825．
［9］Berger C, Day P, Meier G, Zingg W,Bossart W, Nadal D. Dynamics of Epstein-Barr virus DNA levels in serum during EBV-associated disease［J］. J Med Virol, 2001, 64(4): 505-512.
［10］Kasahara Y, Yachie A. Cell type specific infection of Epstein-Barr virus (EBV) in EBV-associated hemophagocytic lymphohistiocytosis and chronic active EBV infection［J］. Crit Rev Oncol Hematol, 2002, 44(3):283-294.
［11］Teramura T, Tabata Y, Yagi T, Morimoto A, Hibi S, Imashuku S. Quantitative analysis of cell-free Epstein-Barr virus genome copy number in patients with EBV-associated hemophagocytic lymphohistiocytosis［J］. Leuk Lymphoma, 2002, 43(4):173-179.
［12］Imashuku S. Clinical features and treatment strategies of Epstein-Barr virus-associated hemophagocytic lymphohistiocytosis［J］. Crit Rev Oncol Hematol, 2002, 44:259-272.
［13］Kimura H, Morishima T, Kanegane H, Ohga S, Hoshino Y, Maeda A, et al. Prognostic factors for chronic active Epstein-Barr virus infection［J］. J Infect Dis, 2003, 187(4):527-533.
［14］Yamashita N, Kimura H, Morishima T. Virological aspects of Epstein-Barr virus infections［J］. Acta Med Okayama, 2005, 59(6): 239-246.
［15］Okan M. Overview and problematic standpoints of severe chronic active Epstein-Barr virus infection syndrome［J］. Crit Rev Oncol Hematol, 2002, 44:273-282.
［16］Ryan JL, Fan H, Swinnen LJ, Schichman SA, Raab-Traub N, Covington M, et al. Epstein-Barr virus (EBV) DNA in plasma is not encapsidated in patients with EBV-related malignancies［J］. Diagn Mol Pathol, 2004, 13(2):61-68.