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高压氧治疗促进HIBD新生大鼠内源性神经干细胞的迁移与分化
王晓莉, 杨于嘉, 谢岷, 王庆红, 余小河
中国当代儿科杂志 ›› 2009, Vol. 11 ›› Issue (9) : 749-752.
PDF(1671 KB)
PDF(1671 KB)
高压氧治疗促进HIBD新生大鼠内源性神经干细胞的迁移与分化
Hyperbaric oxygen promotes the migration and differentiation of endogenous neural stem cells in neonatal rats with hypoxic ischemic brain damage
目的：该研究探讨了高压氧（HBO）对缺氧缺血性脑损伤（HIBD） 新生大鼠内源性神经干细胞（NSCs）的迁移与分化的影响。方法：7 日龄 Sprague-Dawley 新生大鼠随机分为对照组，模型组及HBO组。采用 Rice-Vannucci 方法制成 HIBD 模型。造模后 3 h 内行HBO 治疗。分别于 HBO治疗后 7 d、14 d、 28 d，采用 BrdU/DCX，BrdU/β-tubulin，BrdU/GFAP和BrdU/O4免疫荧光双标法，用共聚焦显微镜动态检测侧脑室室管膜下区（subventricular zone, SVZ）与大脑皮层内源性 NSCs的迁移与分化。结果：治疗后7 d，HBO 组损伤侧SVZ 区 BrdU+DCX+细胞数（84±21 个/mm2）增加，多于对照组（39±14 个/mm2）与模型组（68±17 个/mm2）（P<0.05）；治疗后14 d，SVZ区BrdU+DCX+ 细胞数减少，而皮层区BrdU+DCX+ 细胞数增加，HBO组明显多于对照组(P<0.01）；治疗后28 d，各组大脑皮层BrdU+DCX+ 细胞数减少，大脑皮层出现BrdU+β-tubulin+，BrdU+GFAP+，BrdU+O4+细胞。HBO组BrdU+β-tubulin+与BrdU+O4+细胞数显著高于对照组与模型组（P<0.05）。结论：高压氧可促进 HIBD 新生大鼠内源性NSCs迁移到大脑皮层并分化为成熟的神经细胞。［中国当代儿科杂志，2009，11（9）：749-752］
OBJECTIVE: To explore the effects of hyperbaric oxygen (HBO) treatment on the migration and differentiation of endogenous neural stem cells (NSCs) in neonatal rats with hypoxic-ischemic brain damage (HIBD). METHODS: Seven-day-old Sprague-Dawley rats were randomly divided into the normal control (CON), the HIBD model and the HBO groups (HBO treatment was administered at 2 ATA, once daily for 7 days within 3 hrs after HIBD). HIBD model was prepared according to the classic Rice-Vannucci method. BrdU/DCX, BrdU/β-tubulin, BrdU/GFAP and BrdU/O4 immunofluorescence were examined by confocal microscopy in the subventricular zone (SVZ) and the cortex 7, 14 and 28 days after HBO treatment. RESULTS: The BrdU+DCX+ cells in the SVZ (84±21 cells/mm2) in the HBO group were significantly higher than those in the CON group (39±14 cells/mm2) (P<0.05) and the HIBD model group (68±17 cells/mm2) (P<0.05) 7 days after HBO treatment. Fourteen days after HBO treatment, the BrdU+ DCX+ cells decreased in the SVZ and more cells were observed in the cortex in the HBO group as compared with the CON group (P<0.01). The BrdU+ β-tubulin+, BrdU+GFAP+ and BrdU+ O4+ cells were observed in the cortex, and more BrdU+β-tubulin+ and BrdU+ O4+ cells were observed in the HBO group as compared with the CON and the HIBD model groups (P<0.05) 28 days after HBO treatment. CONCLUSIONS: HBO treatment may promote endogenous NSCs to migrate to the cortex and differentiate into mature neurocytes in neonatal rats with HIBD.［Chin J Contemp Pediatr, 2009, 11 (9):749-752］
Hypoxic-ischemic brain damage / Hyperbaric oxygen / Neural stem cell / Neonatal rats
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